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Showing: 61 - 70 of 1073 results
  1. Plasmids 101: Terminators and PolyA signals

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    Blog Post
    ...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and rrnB,...engineered terminators come close (>95%). For most purposes, however, any common terminator will suffice...suffice. Many commercial expression vectors use double terminators to reduce unwanted translation of downstream...require binding of termination factors. Although both are termination factor dependent, Polymerases I and...Posttranscriptional Regulatory Element Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal...of yeast terminators of varying strength. A discussion of the effects of various terminators in the context...termination and polyadenylation? The role of the terminator, a sequence-based element, is to define the end...
  2. The SGC Toronto Collection

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    Collection
    ...Structural Genomics Consortium Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry... from the Structural Genomics Consortium (SGC) Toronto collection Plasmid...Genomics Consortium Plasmids Browse SGC Toronto collection Structural Genomics Consortium Plasmids Structural...
  3. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post
    ...block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial constitutive...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively high transduction... many more useful viral vectors available at Addgene: Follow our viral vector blog posts for updates on...tips for using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...
  4. PEI Calculator for Planning AAV Packaging Transfections

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    Blog Post
    ... download the PEI calculator for AAV packaging Use the PEI transfection calculator for AAV packaging ...corresponding column in the calculator spreadsheet: We hope that this calculator will make it easier to plan...for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires transfecting...right, you need to do a few calculations. This calculator can do the math for you and it’s simple to make...whole experiment And here’s the equation the calculator uses to give you the μl needed of each plasmid...experiments. Let us know what you think about the calculator in the comments section below. References 1....Production of Recombinant Adeno‐Associated Viral Vectors for In Vitro and In Vivo Use." Current Protocols...
  5. New and Upcoming Viral Vectors - December 2019

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    Blog Post
    ...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have...pAAV.hSynapsin.SF-iGluSnFR.A184S Viral vectors coming soon! These vectors should be packaged and available ... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control...in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters and we are excited to offer...
  6. Six Spooky Science Stories and Halloween at Addgene

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    Blog Post
    ...science stories. Here are some of our favorite spooky science stories. Six spooky science stories Zombie...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways ...Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green...forcing it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then... "Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." ...
  7. New and Upcoming Viral Vectors - September 2019

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    Blog Post
    ...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...Calcium Sensors). We are expanding the inventory of these vectors to more serotypes. We currently package...GCaMP7s. See all our biosensors inventory, and check out our latest vectors below: pGP-AAV-syn-jGCaMP7f-...addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR...support by growing our inventory of recombinases and recombinase-dependent vectors. We recently released...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...
  8. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post
    ...guarantee certain rights and protections for depositor and requestor alike. That’s why Addgene works closely...closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions...California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute  Fred Hutchinson...
  9. New and Upcoming Viral Vectors - June 2019

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    Blog Post
    ...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have...pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics inhibitors Plasmid Serotype Name 99039 ...AAV1 pAAV-ihSyn1-DIO-tTA Viral vectors coming soon! These vectors listed below should be released at... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...few months. You can also browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype...always excited to add new control AAVs to our inventory since we know that scientists need these tools...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid...
  10. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have... used in conjunction with optogenetic activators and inhibitors that respond to blue light. We recently...GABAergic interneurons. These vectors can also be used as the control vectors for experiments encoding a ...pAAV-hSyn-mCherry at Addgene. Viral vectors coming soon! These vectors listed below should be released at... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...far in 2019. You can also browse our entire AAV inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE... Addgene. GABA sensor AAV GABA is the primary inhibitory neurotransmitter in vertebrates, but methods ...
Showing: 61 - 70 of 1073 results