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...ll dive into those questions in this post as we explore… the life and times of the myc tag.  Origins of...that their new monoclonals would help the field explore Myc’s role in cell proliferation and tumorigenesis...

...quickly. Of course, you might have less time to explore scientifically since you can’t afford to spend ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

...super-resolution microscopy. To make this segment of the color spectrum more powerful, the lab of Francois St-Pierre...EGFP, or mScarlet reporters, so you can choose a color compatible with the other tools in your experiment...

...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...concentration of a chaotropic salt (guanidine hydrochloride for the plasmid purification protocol and guanidine...

...techniques to explore dark matter in the genome. There are countless unknown regions yet to explore. I hope ...

...but also alter an individual’s native, beneficial flora. Currently, there is no antibiotic strategy available...population, represented here by cells of different colors, specifically targets bacteria with the given genomic...

...processes. Re-engineering GFP to increase its range of color and application Almost as soon as its sequence... not listed here, many permutations within each color also exist with only slight variations separating...

... she's either day-dreaming about exploring sci-fi worlds or exploring nature in the real world. Additional...

...’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project. Gymnotiformes and Mormyridae...evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the...

... their selection techniques to come up with new colors (Fig. 3), such as Radish and Carrot, and further... ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...

...other disciplines, or from other countries, to explore new connections to our research. So how can we ... 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science journals: An overview...

...into the content of Addgene’s aptamer soup by exploring the development and applications of fluorescent...aptamers Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in...

...experiment and choice of loading control. First, let’s explore why these assumptions are key to using loading ...loading control. Thanks for joining us in this exploration of western blot normalization. May your blots...

...Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan to reuse...about the particular audience and venue before tailoring each presentation appropriately.  Find out who...

...specific nucleotide substitutions (or deletions) in a tailored manner. The approach can be used in conventional...plasmids, but may also facilitate genome editing. Tailored mutations are commonly introduced to endogeneous...

...fluorescence for fluorescently labeled probes. Colorimetric and luminescence based detection methods are...and behavior.     References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments...

...of carbohydrate binding proteins waiting to be explored and exploited. With better characterization, these...Jessica C. Sacher, and Christine M. Szymanski. "Exploring the interactions between bacteriophage-encoded...

... work The Island of Dr. Moreau (1896) where he explores the concepts of animal testing and modifications...but the common themes that these authors were exploring were space and robots. Warping to stars in space...

...chemical reactions and this induces a noticeable color change. This method has high sensitivity but low...Molecular Biology (University of Milan, Italy) and a Bachelor's degree in Biotechnology.       References Degeling...

...graphically depicts the sequence as a series of colored peaks corresponding to one of the four nucleotide...

...effector — Cas9, Cas12, or Cas13. If you’d like to explore Class 2 CRISPR in more depth, the Jennifer Doudna...8(1). https://doi.org/10.1186/s13578-018-0255-x Taylor, H. N., Laderman, E., Armbrust, M., Hallmark, T...

...antibodies (i.e., immunofluorescence), or by a colorimetric reaction facilitated by an enzymatic reaction...Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS, VACCINES, AND IMMUNIZATION...

...Rhodamine 123 (R123) (Liu et al., 2017). In multi-color imaging, it is essential to select dyes with different...applications. Other current advancements include work exploring far-red-emitting dyes in bacterial imaging, offering...

...fluorophores can add a second layer of contrast, color contrast, to an image and thereby provide molecular...Microscopy Choosing Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent Protein...

... the Video! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification...

...mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin 25 µg/mL Gentamycin 10...

... of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting...variety of bioactive natural products. To easily explore and engineer pathways within this genus, Huimin...

... the sample or b) using dyes or stains that add color to some components of the sample but not others....

...appropriate tips, which are made in a number of sizes and colors depending on the pipette they are used with and...

...Traditional tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually...ratios, making these neurons fluoresce in different colors. An individual neuron can then be traced throughout...