We narrowed to 306 results for: SPI;

...in Germany. I attended this symposium seeking inspiration to resolve the technical hurtle of monitoring...

... for nonspecific binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins...

...left with a bunch of random solutions when all the spin columns run out. In this article, we cover the basics...

...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...

...ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our shipping ...

...Python or MATLAB. Choosing Python makes it easy to spin up a read-only Jupyter notebook for them and let...

...system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is also suitable for optogenetic ...

...growing dialogue on the state of a system that, despite all of its successes, has many deep-rooted flaws...

...Check out the hashtag #365papers on Twitter for inspiration and accountability. There are a few different...

...with a penicillin resistance plasmid can grow in spite of a hostile Penicillium fungus trying to kill them...

... careers are in advance of yours, but also get inspired and meet peers. Join societies like National Science...

...measure the tension in the helical structure of spider silk, or the activity of a protein in a synapse...

...ultracentrifugation, centrifugation on a sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation...

...things your lab can do to get into the robotics spirit include: Start using electronic multichannel and...

...currently working on giving this treatment a CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma,...

...for students interested in leading the process despite the recent advance. Similarly, grad students throughout...

...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

...., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A., Sousa, A. A., Tsai, S. Q., ...

... of your baculovirus is straightforward — simply spin down the supernatant in a centrifuge to remove any...

...deposited their plasmid with Addgene, hoping to inspire others to explore the phenomenon, improve the tool...

... Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 144-188 bp 100 ...

...see how low we could go. We prepared a range of spiked samples from 0.1-20% and discovered that, with ...

...dish containing the circled clones is removed by aspiration and the dish left covered (to avoid drying out...

...researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized...

... inactivate specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in...

..., resulting in lower overall genome frequency. Despite the progress we’ve made, there is still a need ...

...Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy...

...postdoctoral training from Massachusetts General Hospital and Harvard Medical School. Connect with her on...

..., or a home-brew optimized for plasmid yields. Spiking your media with supplements such as magnesium salts...

...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, of course...