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We narrowed to 728 results for: STO

Showing: 181 - 210 of 728 results

  1. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...have helped Addgene grow and spread our mission.  Boston Museum of Science, where else? Addgenies and other...honored guests headed to the Museum of Science in Boston, where the entire Blue Wing was set aside for the...refresher on the Central Dogma of Biology, I would stop by later this year when it is displayed!).  The ...Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first). After the speeches,...

  2. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...maintain high standards for both our depositors and requestors.  Requesting Plasmids available for Packaged ...quality control assays we run with all of our in-stock viral preps: ddPCR, purity testing, sterility testing...AAV2 to be 7x1012, the same titers found in our in-stock preps. If a request has technical considerations...considerations that may affect expected titers, a customer support specialist will reach out to you during the request...

  3. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...depositors, used by our requestors, and supported by our repository. You can enjoy these stories, and other 20th...rabies plasmids in our repository to international requestors.    I would be remiss not to mention that 2024...Throughout the year, we’ll be celebrating with stories of innovation and impact made possible by the sharing...

  4. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...applications in plants. This smaller, easily customizable vector functions in multiple species, including...for many applications, the plasmid can also be customized due to its modular structure. Two multiple cloning... regulatory elements to be inserted, further customizing gene expression. Sahoo et al. have tested the...Western blotting. Given its versatility and customizability, pSiM24 can be used not only for plant engineering...

  5. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...surely what is one of many awards that will be bestowed upon discovers of CRISPR/Cas9 genome engineering...panel discussion with Dr. Craig Mello and Dr. Paul Stoffels from J&J. The panel was moderated by a non-scientist...done with this technology to help patients. Dr. Stoffels and others in the audience discussed how J&J is...Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution...

  6. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...is especially exciting, including histone acetylation by p300, histone demethylation by LSD1, cytosine ... subpages, our updated CRISPR Guide and CRISPR History provide a great overview of the development of ...resources: CRISPR Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

  7. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...patient partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ ...watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play with my kids... as efficiently in humans. This is why we cannot stop searching for novel solutions to treat rare diseases...against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many new heroes to the...

  8. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab in the ...supply house.  These large, cheap aliquots could be stored indefinitely at room temperature (thus also saving... powder in 50% nitric acid, but I never let that stop me. I only had to replenish my glass milk supplies... required less agar, less ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  ...

  9. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus... The CellTags revealed that cells with common ancestors tended to have similar reprogramming outcomes....results suggest that iEP reprogramming is not stochastic, but rather there’s a particular cell state that...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris...

  10. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...entrepreneur in the health sciences?  Is this a different story altogether? The unfortunate answer is yes, largely...process. I am the cofounder and CEO of miLab, a Boston-based startup focused on empowering early-stage... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...

  11. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...population (pink histogram) compared to the WT cells (green histogram). The unfilled histograms show cells ....3995 Virk, H. S., Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, ...Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P...

  12. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...lab meetings with a focus on discussion and brainstorming Though, we might be forced to work from home...of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira.   Implement new...can be used as a break, but can also be used to brainstorm on data in smaller groups, where for instance...

  13. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...great deal more information that one might like to store about FPs than is currently represented at fpvis...a centralized, community-editable resource for storage and dissemination of information about FPs. FPbase.org...Users can “favorite” certain proteins, or create custom protein collections (such as a set of FPs that ...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

  14. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...resources at Addgene. His background in history and specifically the history of RNAi left him a bit skeptical...Listen to the interview here! Doench majored in history during his undergraduate work at Hamilton College...just been discovered, the mechanism was barely understood.” Doench’s graduate work helped expand our understanding...

  15. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...our guest bloggers Joachim Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...

  16. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...to package reprogramming factors. An important milestone of reprogramming is the silencing of reprogramming...involves making chimeras by injecting iPSCs into blastocysts and implanting them into female mice. Generating...complementation, involves injecting tetraploid blastocysts with iPSCs and measuring their ability to direct...PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving field of induced pluripotency...., and K. Hochedlinger. "Induced pluripotency: history, mechanisms, and applications." Genes & Development...

  17. Antibodies 101: Conjugation

    Type
    Blog Post
    ...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...Hegazy, et al., 2020; Niemeyer, et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for ...–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous...resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies 101: The Basics of Western...

  18. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ...fluorophore will bleach. This is, however, not the full story. The photobleaching rate does not depend on excitation...our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...

  19. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry may all use either...In the indirect approach, you can simply keep a stock of conjugated secondary antibodies on hand and use...signaling molecules. Many labs find that a trusted stock of secondary antibodies saves time and money when...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals...

  20. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...breast tumorigenesis: Friend or foe?." Histology and histopathology (2018): 18021-18021. PubMed PMID: 29978449...enzymes and metabolism reprogramming aren’t the whole story. It’s essential for a tumor to spread and to resist...sensitive ones. Though this process is not fully understood, a team of researchers lead by Takayuki Kogure...

  21. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo Colombi...probably time to prepare new plates with a fresh stock of antibiotic. Many small colonies surrounding big...University. Oskar Laur, PhD runs Emory University DNA Custom Cloning Service since 2009. He has  developed a...he has used for cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds...

  22. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...presence of Cre, the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is...with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest...is from Tyler Jack's lab: Lox-Stop-Lox TOPO.  Cre-dependent gene knockout – conversely, putting the loxP...

  23. Working with Nuclear Receptors

    Type
    Blog Post
    ...efficient energy maintenance - through acquisition, storage, and utilization - and on self-propagation by reproduction...diabetes, cardiovascular disease, cholesterol gallstone disease, senescent illnesses, and the metabolic...other members of the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. ...sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors, and making them available...

  24. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...a nucleus -> DNA compaction around histones -> Examples of histone-modifying enzymes and reader-effector...repository that will help epigenetic researchers custom design their own engineering plasmid tools in about... specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these modules...

  25. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...blogger Clare O'Connor an Associate Professor at Boston College. National reports stress the importance...yeasts are thought to have diverged from a common ancestor ~350 million years ago. Over time, the two genomes... that is part of the biology core curriculum at Boston College. Students in the course work in teams of...biochemistry, molecular cell biology and genetics at Boston College. She is particularly interested in strategies...

  26. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...Stanford develops engineered DNA systems capable of storing data and computing inside living cells. He's also...describing the development of “transcriptors,” transistor-like digital genetic switches that enable cellular...the beginnings of computational logic and data storage. What we don't have are the keyboards and displays...mining. There is an absolutely incredible hidden story here. The parts we are releasing simply plug into...

  27. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...unevenly, introducing distortions in your final images. It is possible to keep distortions to a minimum with...done about that.  Second, expansion introduces distortions. The goal in expansion is for your sample to ... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

  28. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... convey enough information to tell a compelling story in an image or do you need a full exhibit? In a ....  Static. Can be difficult to convey an entire story or context via still images. Some things cannot ...information. Primarily useful for data-focused stories. In-person lectures, presentations, etc… Low...interested in scicomm. Difficult to do in-depth storytelling. Requires regular content production. Internet...

  29. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency (x-axis) which SNPs ...ancestry-based bias.   The correction process does not stop there. Currently, they are working with Broad's ...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...

  30. Technical Design of a Western Blot

    Type
    Blog Post
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...
Showing: 181 - 210 of 728 results