We narrowed to 400 results for: pho;

1. Understanding GPCR Signaling Using TRUPATH

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   ...FRET), in which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET...

2. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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   ... to students, like how to pour a gel [for] electrophoresis or doing a dissection video. I did that in ...themselves. “You open up the box and there’s a gel electrophoresis tray in there. Or the dissection specimens....

3. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   ...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...

4. RUBY-Red Siliques

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   ... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby The ease of observing ...

5. Antibodies 101: Chimeric Antibodies

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   ...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...

6. New and Upcoming Viral Vectors - September 2019

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   ...scientists access to a wide array of promoters and fluorophores for validating and testing purposes. We will...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...

7. Plasmids 101: Gram Negative and Positive Bacteria

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   ...Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559 For more specific procedural...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...

8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   ...these TFs to see if the differentiated cells had morphologies consistent with the predicted cell type (e.g...neuron predicted differentiation), and 15/17 morphologically aligned. Documentation of the network that ...

9. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...transmission within minutes. Constructs using the photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin...gas-filled protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins ...

10. Antibodies 101: Flow Cytometry Controls

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    ...should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

11. Plasmids 101: Inducible Promoters

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    ...sensing protein YFI. When light is absent, YFI phosphorylates FixJ, which binds to the FixK2 promoter to ...

12. Sweating the Small Stuff: Details in the Lab

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    ..., a large set of headphones can act as a Do Not Disturb sign, even if the headphones aren’t actually playing...

13. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    ...tumorigenesis assays, drug epistasis assays, and morphological profiling can be used as generalizable strategies... are described in this paper Systematic morphological profiling of human gene and allele function ...

14. Multiplex Genome Editing with CRISPR-Cpf1

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    ...example of how this works. Make sure to order 5’ phosphorylated oligos or treat with T4 PNK. Delivery options...

15. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    ...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...cascade. This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene...

16. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ...inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of bi-stable anion... medicine, evolution of body plans, synthetic morphology, and even artificial intelligence," says Michael...

17. Antibodies 101: Affinity Reagents

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    ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    ...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...

19. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...costs low. Most will work with you remotely (by phone, Skype or Google Hangout) but in-person coaching...White Consulting frequently do sessions by Skype or phone. Samantha Sutton is another very experienced and...

20. Working with Nuclear Receptors

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    ...actions of the whole complex.  For example, the phosphorylation status of SRC-3 defines its association with...

21. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    ...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

22. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    ...contact information, and potentially a photograph of yourself. A photo can be very useful in the event that...

23. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...frequent CDG type characterized by deficient phosphomannomutase (PMM) activity due to mutations in the PMM2...

24. Plasmids 101: FLEx Vectors

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    ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...for clearer characterization of the location, morphology, and circuit mapping of a targeted neuronal population...

25. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...taking a snapshot of chromatin looping with the photo “developed” by analyzing DNA sequencing data. While... The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE 2.0 allow us to uncover...

26. Magnetic Control of Proteins: More than a Dream

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    ...responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...

27. Pooled Library Amplifications

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    ...Screening Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 ...

28. Plasmids 101: Protein Expression

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    ...commonly used promoters include the phosphate and copper inducible PHO5 and CUP1 promoters respectively. ...

29. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed cell...

30. Antibodies 101: Producing Recombinant Antibodies

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    ...Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers have an...