We narrowed to 808 results for: Eng

...First Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. “DNA-guided...efficiency. Smaller size At only 887 amino acids in length, NgAgo is shorter than SpCas9 (1368 aa), SaCas9... High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional Resources on Addgene.org Check...

...antibody selectivity. Additionally, it can be a real challenge to troubleshoot RNAi failures. Potential explanations...cell survival or proliferation, an additional challenge is that only partial or ephemeral knockdown will...confirmation of antibody selectivity even more challenging. Correlation with RNA or Proteomic data from ...supportive of their specificity for the protein. A challenge to this approach is that the identity of the epitope...the specific sample and protocol. This can be a challenge to confirm. As such, the approach used may need...

...limited to biotechnology, chemistry, architecture, engineering, mathematics, physical sciences, social sciences...your company employs at least 3 full-time people engaged in researcher activities. The EB-1B requires that...algorithms to program self-driving cars, tissue engineering for organ replacement).  In addition to presenting... and translations of documents that are not in English. Create a Google Scholar account Sanitize your ...meets the requirements set forth by USCIS before engaging with clients. To learn more, visit www.casseuslaw.com...

...presenter, it can be difficult to keep the audience engaged through a computer screen. For the Reproducibility...organizers found several avenues to keep the audience engaged. They first sent a pre-workshop questionnaire to...are still other ways to make your session more engaging, for example, by using short animations and visuals...poster,” he says.Viewing a poster on a screen is challenging, requiring you to zoom in to different parts ...

... we understand such a structure well enough to engineer it to suit our purposes? In search of simple ... we understand such a structure well enough to engineer it to suit our purposes? These questions can be...2012). This made them an appealing target for bioengineering, but it wasn’t clear whether they could 1) ...bodies We have shown that all three of these engineering objectives are possible in our recent paper (...

...supported this project with funding. Alnylam and New England Biolabs sponsored the capital construction of our... solicited seed money for the new project. New England Biolabs and Alnylam see the value to the scientific...research and discovery, while also pushing us to challenge assumptions, holding us to nonprofit best practices...reach scientists around the world. Our Software Engineering Team (and, added in 2018, our Product Team) continues...

... from audiences as they do science outreach and engage with the public, and learn from the process of ...of science, communication isn't easy.  But the challenge is worthwhile and rewarding. A truly passionate...organizations. At ComSciCon, our primary mission is to strengthen and support the network of graduate students ...Learn about careers in science communication The strength of story telling Science communication: 9 strategies...

...below 4.4kB (with the ITRs bringing total genome length to ~4.7kB.  If your insert alone is 4.2 kB and ...serotypes such as AAV9 or AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent...prohibitive problem? A: Re-administration is a challenge for AAV due to the development of neutralizing...neutralizing antibodies in animal models. All of our engineered AAV are presently built on an AAV9 backbone and ...

... Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...cell type, amount of protein, and desired fragment length for your output assay. A longer sonication time...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

...longstanding challenge. Using shRNA, TALEN, or ZFN to edit immune cells poses challenges such as the inhibition...Salmonella Typhimurium. This work demonstrates engineered gas vesicles as the first reporter genes using...sgRNA insert site and a reporter for tracing the engineered cells in vivo, the non-immunogenic violet-excited...

...professional development, and while it’s not challenging to use, it’s hard to know where to begin. To ...helped her connect to other black scientists and engineers on Twitter, but also led to interviews on Al Jazeera... statistics on what percent of scientists and engineers regularly use the platform, why scientists are...media, but how you use social media to consistently engage with others about your topic(s) of interest. Hopefully...

...but where the kinases are located, their signal strength, and how long their signals last all impact transduction...the signal must be transmitted by intermediate messengers. Optical control of kinase activity can provide...preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control...

...itself. In their CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences...permit labeling, but a newly expressed standard length gRNA would “switch” the system and induce genome...Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34...Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for genome editing, activation and repression...

... immense task (it takes, ooh, approximately the length of your average PhD to achieve!) Each field has...there. In every project, time, attention, and/or length restraints mean you will have to pick and choose...cognitive load changed the way I thought about engaging with learners. If something I was communicating...accessible pieces of communications that drive engagement with your target audience. Thinking about cognitive...

...drawbacks of the indirect method, lengthening an often already lengthy process. (And while I have it on...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...has introduced new options for increasing signal strength.  For the time being, though, secondary antibodies...

...scientists can also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take...take a look at a few of these methods below. Engineering gut microbes in situ While the ability to monitor...References Leonard, Sean P., et al. "Genetic engineering of bee gut microbiome bacteria with a toolkit...PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature...

...year (12% in 2018) since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere...happening. The Addgene team will have some fun challenges this year and I’m excited about this, but definitely...documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. We are blessed with a ...appearance and functionality are happening and this strengthens our image in the world. Addgene is now as much...

...improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered Cas9 nucleases...-target specificity of the Cas9 nuclease with engineered variants: eSpCas9, SpCas9-HF1, & HypaCas9. ...Plasmids Browse our CRISPR Guide Pages Find Genome Engineering Resources ...

... preliminary results (Dimitri et al). CRISPR engineering of CAR-T cells is particularly promising because... the first-ever systemic CRISPR therapy. They engineered a CRISPR-Cas construct targeting the diseased...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...2013). Dimitri, A., Herbst, F. & Fraietta, J.A. Engineering the next-generation of CAR T-cells with CRISPR-Cas9...

...tests.  Labs including Jennifer Doudna’s lab and Feng Zheng’s lab had already developed nucleic acid detections...resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis E, Catamura ...NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein...

...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology...one take dried seahorse for?), but there were no English speakers around. Opening talks – Genomics discoveries... sensitizing gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the...cas9 proteins from different species for genome engineering. The final keynote presentation was by Nobel ...

... to be prepared to take on the challenges and be proactive and engaged once you are in a leadership role...online and on monthly phone calls. This has its challenges as meeting in person is often much more productive...different types of people in order to harness their strengths and work effectively. Leadership responsibilities...

...program itself. Picking a mentor comes with many challenges, and the flood of interview requests and visitation...logistical, financial, etc…) now have options to fully engage in the application process. And even if you intend...a travel day to an online call in response to challenges on their end. Though the quick changes in plans...using the virtual world to your advantage can strengthen your application and help you decide if a program...

...this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable expression..., though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one...Harnessing type I CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12),...CRISPR-Cas Systems INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources...

... Biology (Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their...experience on their resumes). CUREs are not without challenges. Real research is messy, and it can be difficult... value of this approach and encouraging them to engage in the problem-solving process. His previous experiences...research experiences are a “CURE” that increases engagement by students and teachers. FEMS Microbiology Letters...

...Nowadays a toolbox of naturally occurring and engineered AAV serotypes that further expands the range ...currently used in clinical trials, a variety of engineered ones have shown great promise in preclinical ... AAV safety profile is excellent and further strengthens its position as the vector of choice for in vivo...being done to further improve the technology: engineering of new capsid variants, designing expression ...

...immunodetection tools is their strong signal strength. This signal strength is due in large part to their larger...requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For...labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (Pleiner ...

...., Zhang, Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and... reduce your lab’s waste footprint. The Zhao and Deng labs developed a simple method for regenerating ... this many uses of the columns, but the Zhao and Deng labs found they could just make their own buffers...

...this case, the absorbance of light at three key wavelengths: 230 nm, 260 nm, and 280 nm. Nucleic acids exhibit...characteristic absorption spectra across this range of wavelengths with maximal absorption at 260 nm (Figure 1A)...the sample also absorb light in this range of wavelengths (Figure 1B-D). These other molecules can alter...A260/230 ratio of a DNA sample, its absorbance strength is low compared to other reagents, making it more...

...may provide a method for doing just that. While challenges remain in the delivery of these agents, CRISPR...potential clinical applications, Ando et al. recently engineered synthetic phages, each based on the well-studied... Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial...Addgene Blog CRISPR Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene ...

...libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated by the Feng Zhang lab, this is...presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through how...activation screening - An excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production...

...the level of target antigen in a sample. While Engvall et al. originally developed the ELISA assay to ...small molecules from a variety of sample types (Engvall 1971). Scientists value ELISAs for their accuracy...antibodies. Assay development, however, can be challenging since scientists must first identify two independent...Longer protocolIncreased chance of cross-reactivityChallenging to develop assay Competitive Appropriate...

...amounts of target proteins or antibody bonding strengths.    . Steps of an IP: 1) Protein extracts...Protein G have an affinity for immunoglobulins, the strength of the interaction varies between antibody host...many factors, including salt concentration, ionic strength and pH, affect binding the lysis buffer may need...step. The wash buffer has an optimal pH and ionic strength that breaks weak interactions from residual non-specific...

...for a liver transplant. My lab will combine bioengineered scaffolds with patient stem cells to generate... in your chalk talk, there's the vital task of engaging your audience. You need to make your audience ...search committees. If possible, do at least two full length practices (~1 h) and get feedback. It’s also helpful...outside your field. If they can understand and are engaged by your presentation, then you’re on the right ...

...to an adenine base editor. A massive protein engineering effort by Gaudelli et al. resulted in the successful...colonies and subsequent rounds of evolution and engineering gave rise to a mutant TadA (TadA*) that satisfactorily.... Kang, Beum-Chang, et al. "Precision genome engineering through adenine base editing in plants." Nature...CRISPR-mediated base editing in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor...

... Rett Syndrome, respectively. One of the main challenges facing Rett researchers is how to deliver the...depositors David Liu, PhD, Keith Joung, PhD, and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for... by Gail Mandel. There are numerous technical challenges to overcome for both of these approaches, such...abstract Sinnamon JR, Kim SY, Fisk JR, Song Z, Nakai H, Jeng S, McWeeney SK, Mandel G (2020) In Vivo Repair of...

...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...Howes, Rob, and Christine Schofield. "Genome engineering using adeno-associated virus (AAV)." Chromosomal...Benjamin E., and David V. Schaffer. "Combining engineered nucleases with adeno-associated viral vectors...editing." Precision Medicine, CRISPR, and Genome Engineering. Springer, Cham, 2017. 29-42. PubMed PMID: 29130152...

...natural inclination for this skill that you could strengthen. The right set of transferable skills, coupled...lack thereof): Information sharing gets more challenging the bigger an organization gets, and creating...conflict resolution is likely not one of your strengths. Empathizing with people, especially people with...recognize that conflict resolution may never be a strength for you. But if you are a problem solver, and ...

...16568824. Hmelo, Laura R., et al. "Precision-engineering the Pseudomonas aeruginosa genome with two-step...PMID: 31201277. Liljeruhm, Josefine, et al. "Engineering a palette of eukaryotic chromoproteins for bacterial...bacterial synthetic biology." Journal of biological engineering 12.1 (2018): 8. PubMed PMID: 29760772. PubMed...Thomason, Lynn C., et al. "Recombineering: genetic engineering in bacteria using homologous recombination." ...

...sequence in the way that they like, the design and engineering of fusion proteins is relatively straightforward...properties of the protein of interest can be challenging. In this blog I discuss strategies to generate...before generating a fusion protein, e.g. linker length, the specific fluorescent protein variant, and ... (catalytic activity or interactions) can be challenging. If a fusion can complement a knock-down or knock-out...

...for investigating neuronal signaling and for engineering improved sensors for these and other targets....this in mind, Ed Boyden and colleagues set out to engineer a potassium ion biosensor with a binding affinity...sensors. Using genome mining and structure-guided engineering of potassium binding proteins, they developed...and are a promising starting point for further engineering. Figure 5: Structure-guided design and genome...

... validated first through the production of full-length protein (the TAG codon should encode phosphoserine... instead of a stop codon, which will allow full length GFP to be translated), and second, through a gel...for parameters such as induction temperature and length of induction, which will vary between different...phosphorserine has actually been incorporated into the full-length protein. Up to 80% p-Ser incorporation into E17TAG...

...on. In addition, researchers have created many engineered serotypes by mixing, matching, and modifying ...summarize briefly: AAVrg is derived from AAV2 and engineered to infect neurons and undergo retrograde transport...Nagarajah, R., Vitale, J., Wong, A. C. H., Gokal, D., Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song...W., Liu, X., Cai, Y., Kou, J., Wang, J., Li, L., Peng, S., Xu, Z., Zhang, W., Qiu, Y., Wu, Y., Ye, C.,...

... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...straightforward, and is a testament to how far genome engineering has come. For comparison, in 2007 it took about...lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide... genome engineering...

...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...for a successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience...data needed to rule out alternative models or to strengthen your new model?  PFs need to be regularly thinking...and answering questions. Organizing a thoughtful engaging talk takes significant effort. It requires evaluation...

...selection. Knock-in events tend to be rare, and the challenge with any experiment is in efficient recovery of...Toolkit and SapTrap-SEC kit for C. elegans genome engineering have also facilitated CRISPR editing in C. elegans...Higgins CD, Goldstein B (2015) Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette.....113.156075 Ward JD (2014) Rapid and Precise Engineering of theCaenorhabditis elegansGenome with Lethal...

...possible by the light: when hit with the correct wavelength, microbial rhodopsins enable ions to flow across...a laser. The laser flashes light of specific wavelengths, selectively turning neuron activity on or off...1977): 237-243. PubMed PMID: 20882. Mei, Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics...28772120. PubMed Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic...

... cytokines like TGFβ and IL-6. However, when strengthened through efforts to boost the activity of the... form of immunotherapy in recent times. These engineered T cells are capable of recognizing targets like...the above therapeutics, techniques like genome engineering have played a huge role. For instance, researchers...at Binghamton University. She works on genome engineering and cancer immunotherapy, and is interested in...

...-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production of functional...are between 5-8 amino acids long, with a typical length of 5 or 6 amino acids (Cruse et al., 2004). Because...natural immune system production and many more engineered methods. Each type of antibody and production...

...the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids in our ...Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...