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We narrowed to 929 results for: KIN

Showing: 301 - 350 of 929 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...like multi-color imaging (Figure 2). If you’re working with purified proteins in vitro, you can even use...tags makes updating your labels easy! Instead of taking the time to clone a new construct for every new-and-improved...time points.   Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins...developed over the years; improving the labeling kinetics or the fluorophore’s microenvironment to enhance...A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M., Beaufils, F., & Johnsson, K. (2008). An ...Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling...Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic...

  2. How to Lead a Great Meeting

    Type
    Blog Post
    ...something difficult, be excited about the prospect of working toward a solution. At the end of the meeting, make...person to add a bullet or two about what they are working on. Building this into the agenda ensures that ...items, and Accountability Agendas: Send around talking points for the meeting a day in advance. This gives...Accountability: At your next meeting, start by checking in on the action items from last meeting. Use ...project moving. Use time wisely Meetings are for talking, not reading: Send around relevant documents, like... Executive Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside Addgene Posts...

  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...the day you defend to start thinking about your next step. Start thinking now. Start browsing job postings...in your resume. If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers...groups? Perhaps you had some supervisory experience working with undergraduate students? On top of that, perhaps...universal. Some universities even have officers working specifically to assist graduate students in job...postings and salary expectations public, so start by looking at these university postings to give yourself a...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...you build your lab team and foster an effective working environment? Tom Ellis was offered a start-up package...ideas, and ways to improve the lab. “An effective working environment for research is totally dependent on...communication amongst the team so getting everyone talking to each other cannot be undervalued.” Connie Cepko...of slowly building up your lab and selectively picking the first couple of hires to build a strong, collaborative...resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc... Dr. George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists...

  5. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... these files to your working directory (the folder that you are currently working in) every time you use...notebooks just won't cut it for bioinformatics. All kinds of biologists are finding themselves using computational... task will keep you from repeating your work or making similar mistakes in the future. Stay organized...comment in C++ and Java Command line history: Tracking command line history by recording all commands...system that does is through trial and error, and by asking for tips from others. Comment below to add your...

  6. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...Blog Mentoring for Scientists Series. I have been thinking a lot about Mentoring for over 10 years. Many ...Cepko to learn about her mentoring style. While working on this project, I read the book “Every Other Thursday...Form your own peer mentoring group In my travels speaking to grad students and postdocs I have started to...example, a group of people who are all interested in making a transition. Schedule your first meeting and get...breakfast once a month. All participants must commit to making the meetings a scheduling priority (and last minute...career paths and get their advice. Attend a local networking event together and help each other practice meeting...

  7. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ..., consider making you own DNA ladders with these plasmids from Penn State Need help picking the right ...cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell culture media. Their...HA staining is weaker than reporter staining. Picking the right reporter for your RANBody While all three...staining GFP, RFP, H2A2B   Are you ready to start making your own RANbodies? Great! All of the RANbody plasmids...learn more about nanobodies Additional Resources Looking for primary monoclonal antibodies to use with the...

  8. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...heavily with other skills such as critical thinking, decision making, creativity, or data analysis. Conveniently...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...you spend time in a lab learning techniques and taking on independent projects, you will inevitably develop... skills passively. But I have always found that taking a conscious or active approach can be hugely helpful...are, while also practicing systematic, logical thinking.  If you are already a lab veteran at this point...

  9. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ... Research. On December 1st 2016, many postdocs working more than 40 hours per week could expect to see... salaried workers receive overtime payment for working more than 40 hours per week increasing from $23,660... postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...going ahead with their planned raises, some are taking the opportunity of the lapsed mandate (or are being...University of Massachusetts Medical School. We are working with postdocs at other institutions to see if we...that many postdocs already made plans and budgets working around issues such as childcare using money from...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    ... be useful. Mainly because, well, antibodies are kind of hard to see. To solve this issue, researchers...post, where you can find some additional tips for working with these tools.          Enzymes An equally, ...properties allow for efficient signal amplification, making biotin conjugated antibodies good options for detecting...lead perfectly blissful, productive lives without thinking about these chemical reactions. After all, there... are ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...antibodies’ performance after the reaction.  If you are looking to conjugate antibodies yourself, there are kits...

  11. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc in January...after treatment with EGFR inhibitor, erlotinib. Looking at barcode abundances among multiple replicate ...Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor clonal...their own work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and...is a “really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to ... to use! Many thanks to Carrie and Novartis for taking the time to talk with us and for providing this...

  12. Engaging with science and society at pgEd

    Type
    Blog Post
    ...the hopes and concerns of these communities. This kind of engagement and two-way dialogue is crucial if...advising writers and directors in Hollywood; and working directly with community organizations, libraries...leadership and communication experience. These included taking extra coursework and attending seminars in bioethics...writing science articles for a lay readership, and working with middle and high school students. One of these...organizations was pgEd. After obtaining my PhD and working in digital academic publishing for a year, I’ve...and in helping you decide whether you enjoy this kind of work. It is also beneficial to hone your non-...

  13. Lab Automation at Addgene

    Type
    Blog Post
    ...deposits per month!), manual transformations were taking a lot of time. Enter the Biomek FXP! We wanted ...improve our transformation protocol and, after looking at a few options, we decided on Beckman Coulter...protocol on the Biomek FxP is pretty simple. By clicking a few buttons you can quickly tell the Biomek ...pick up 8 tips and position the tips indepently making it easy to spread out your samples. This can be...scientists share plasmids across the globe, we’re working on ways to send plasmids as DNA as opposed to in...it suits our plasmid prepping needs perfectly. Working with Qiagen, we developed a protocol to run up ...

  14. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...proteins had to be re-engineered for each gene, making wide-scale gene activation seem next to impossible...activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without...there are multiple options to choose from when looking to activate genes across diverse cell lines.   ...activation compared to other CRISPR activators, making it a popular method for gene activation experiments...design (SunTag). This streamlines its delivery, making it a common choice for CRISPR activation. Newer... Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-Guided...

  15. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working four ...prepare 1-2 unique questions for each interviewer by looking at their LinkedIn profiles. This can help spark...your 1-hour presentation to your audience Start by asking the hiring manager or recruiter what presentation...improving a process or procedure, solving a problem, or making a key decision. Even deciding to stop pursuing ...sharing your screen and won’t be able to see who’s asking a question. Maybe you ask for questions after completing...

  16. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ... (and Addgene depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab...the majority of attendees were scientists still working at the bench. It was a fantastic opportunity to...cell membrane. His recent work shows that they are making fairly remarkable progress on establishing a system...the drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics. These... CRISPR resources Read Joanne Kamens' Post on Networking at Scientific Conferences  ...

  17. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... gRNA using restriction enzyme BsaI. If you’re working in Drosophila, a two-gRNA expressing plasmid is...outside of their recognition sequence, creating flanking overhangs. These overhangs can be customized to...vectors each contain Type IIS restriction sites flanking the promoter-gRNA construct, but with different...with the appropriate Type IIS enzyme, the unique flanking overhang sequences can link together to allow ...constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors like... mammalian option described below, plasmids for making polycistronic gRNAs are also available from the... tyr gRNA, which causes hypopigmentation, thus marking cells that have undergone genomic modification....

  18. Common Injection Routes in Mice

    Type
    Blog Post
    ...between skin and muscle 25–27 < 2–3 mL (~1 mL per site) Beginner Intradermal Anesthesia Between skin layers...Subcutaneous (SQ or SC) injections are given between the skin and the muscles. The most common injection sites... (ID) injections occur between the layers of the skin. While this could theoretically be performed anywhere...shaved first. Restraint: anesthesia Location: between skin layers Gauge: 26 Volume: < 0.05 ml Skill required...cringe or your experiment will allow you to avoid poking the mice, these “no needles” techniques may come...

  19. Of Myc and Men

    Type
    Blog Post
    ...mentioned above basically involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds...tagging strategy, another group of researchers was working on isolating monoclonal antibodies to better probe...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising...same antibody developed almost 40 years ago? Well…kinda. In some cases, antibody providers are distributing.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

  20. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications...others will avoid it like the plague. Instead of looking for The One Method to Rule Them All, consider the...embark on a project, do your best to understand what kind of resources - including your time - will be required...Podcasts Medium Easy for audience to engage while multitasking. Easy to host guests remotely. Easy access from...things to be physically interacted with. Public speaking is a common fear. Can be discouraging to build...

  21. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...large-scale CRISPR screens in cancer cell lines, looking to uncover genetic dependencies of individual tumors...dependencies across ancestry groups (right).    Seeking an explanation  First, they looked for a biological...unexpected discovery, which didn’t make sense in any kind of biological framework, suggested to them that ...information they needed to begin correcting the issue. Working with James McFarland, Jeremie Kalfon, Javad Noorbakhsh...CRISPR guides mapped to a SNP would be ignored. Working with Lia Petronio and Katherine Huang from Broad...process does not stop there. Currently, they are working with Broad's Genetic Perturbation Platform to modify...

  22. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...in “crispr” and see what comes up. Before even clicking the ‘Search’ button a new content-aware suggestion...jump directly to the type of information you are looking for in our catalog or educational resources: plasmid... your lab. Instead of starting a new search or clicking through to a different part of the website, you...been deposited? In this example we’re going to be looking for recombinant monoclonal antibody plasmids from...finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where...functionally (e.g. Base Editing) and by target organism. Clicking through to RNA Editing brings us to a page with...

  23. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...employees spent this remaining time by elaborately pranking each other, dressing in costume, and holding competitions...the question, “What’s your favorite part about working at Addgene?” Most Addgenies reply with an enthusiastic...collaborators have completed their work thoroughly. It’s kind of a chicken and egg situation- do we work so hard...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all ... To keep the organizational side of things from taking too much of your time, scroll down for some tips...fall into that rut! We’ve done everything from kayaking, to field day, to relay triathlons, to 5Ks. Many...

  24. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...and feel the satisfaction of checking off each step as you painstakingly complete your experiment – one...Addgene Website: http://www.addgene.org/ If you’re working with plasmids and you decide to share them using...of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA.../ Labguru is a good place to go if your lab is looking to streamline your lab management and you want ...reagent storage and inventory, managing orders, tracking students and research projects, electronic lab...

  25. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...modulating the production of the pro-inflammatory cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process...reflect elements of drug addiction and craving (‘seeking drugs’).  What’s involved in locomotor sensitization...protected) rather than being out in the open (risky). Taking advantage of these characteristic traits, rodents...reduced anxiety levels and a higher tendency for risk taking. These animals also show signs of neuroinflammation...

  26. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...consider a dual-sgRNA system. This system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic...deleting the catalytic residue of a protein or knocking out a very small peptide. In these cases, you ...xCas9 has a PAM requirement of NG, GAA, or GAT, making it a more flexible cutter with the added bonus ... You can achieve your precise edit without even making a break.  Base editors are dead Cas9 (dCas9) or...sites was sufficient to repress transcription by blocking its initiation. In mammalian cells, robust repression...interest. dCas9-based repressors are useful when knocking out a particular gene isn’t feasible. For example...

  27. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...chat is a convenient alternative for asking a quick question or making initial contact, but is less suitable...to look forward to in a technical support role Talking to customers everyday, it can be fascinating and...right place to live it out, but ideally without asking too many (annoying) questions. Identifying and ...understanding of when to use these communication skills by working with people from many different backgrounds and... other roles in industry. Many thanks to Eric Perkins and Stephanie Hays for images used in this post...

  28. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ..., 2020.   Scientists around the world have been making major improvements to CRISPR technology since its...blue light is readily absorbed and scattered by the skin, so it is not able to reach deeper tissues. Additionally... however, can penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye...By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...turn on transcription, label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned...spatial control of Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data...techniques (Matony et al., 2020). Whether you are looking to activate, repress, or modify a gene, you now...

  29. Five Popular Model Organisms

    Type
    Blog Post
    ...model organism in scientific research. Yes, the same kind we use in breads and other baked goods! Yeast is... the study of neurological diseases, such as Parkinson’s disease (PD). PD is primarily characterized by...C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments over multiple...60% of human genes have an ortholog in C. elegans making it ideal to study basic molecular biology processes...resources check out Addgene’s Worm Expression page. Looking for more model organisms? Check out Part two in...From the baker to the bedside: yeast models of Parkinson's disease." Microbial cell 2.8 (2015): 262. PubMed...

  30. March for Science

    Type
    Blog Post
    ...politics? Science is an apolitical process for seeking knowledge. The process begins with a testable hypothesis...experiments can help support or reject a hypothesis. Looking at the data, scientists then revise their hypotheses...scientists who hold a doctorate degree, are currently working full-time, and have received a research grant in... of the new administration’s actions, including taking down the climate change page on whitehouse.gov,...depending on the number of people supporting it.  3) Linking scientists to one particular political party while...politicians and citizens to include science in decision making, it will be considered a success. Below are resources...

  31. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...without restructuring. Positive Points for Trainees - Taking Steps to Achieve a Successful Career Career Advice...various career opportunities through our career, networking, and management for scientists blog series. We...Session focused on finding this information and making it more easily digestible/available. What I viewed...welfare of the many researchers out there who are making great progress on some of the world’s most profound...At Addgene we hope that all parties involved in making academic research a successful and enjoyable path...Blog Post Read Our Career Blog Posts Read Our Networking Blog Posts ...

  32. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...delighted to see enterprising early career scientists taking an active part in creating their own opportunities...opportunities for career development and networking. Aside from the importance of the topics to be discussed, ...building valuable skills and meeting new people by working together on the event planning and execution. I...seeing many similar examples of science trainees taking a proactive role in their non-bench training. Grad...all. For grad students and postdocs interested in taking matters into their own hands, there are some easy...

  33. A Control for All Seasons

    Type
    Blog Post
    ...you are studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an...group of mice, the experimental group, with the new kinase inhibitor and a second group, the positive control...control group, with an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You ...experimental protocol but does not receive either protein kinase inhibitor. Since the negative control group does...variability. If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better...noise. This can be done either by modifying the blocking conditions or by substituting for a more specific...

  34. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...RNase free” and refrain from talking to friends and colleagues while working with these reagents. Other ...they are relatively small and easy to work with, making them the go-to choice for studying mammalian biology...technique is still in need of improvement. Also when working with CRISPR, one must beware that not all gRNAs...blog post. Finally, always remember that you are working with RNAs, which are prone to degradation by the..., Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing with Adeno-Associated...

  35. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...honing your science communications skills and networking. And on social media, you could be sharing your...Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting jobs...have to create opportunities. If you find yourself asking, "Why doesn't this product/organization/position...position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew.... Usually when I see inspiring artists who are thinking about getting into the arts (or any career to ...

  36. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ... communicate effectively is part of what makes working in a team productive. Take all the opportunities...conferences on interviewing, resume writing, and networking Practice your English if you need to, go to ESL... a content-useful website, write a blog Pursue speaking invitations by inviting others (be on a speaker...“learn the business” Attend events with local networking and trade organizations Another excellent perspective...People Wish They’d Learned Sooner by Peggy Klaus Looking for a mentor or want to start a peer mentoring ...

  37. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...offers all of those same advantages to researchers working in plant model systems. “The cloning is all done...trade Lohmann does have one special tip for anyone looking to try this at home in their own lab: be very careful...along at 99 percent efficiency, simply stopped working. They finally traced the problem back to the endonuclease...in the process. “We had several motivations for making GreenGate available through Addgene,” Lohmann said...system, the more building blocks will be available, making it easier for us to do our work.” References 1...

  38. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ... Something that I struggle with as I’ve been thinking about my career options is being an overthinker...overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping forward but I have learned...in a life sciences career but life has a way of making unexpected decisions. As someone who was not necessarily...what my Instagram posts would look like. While working remotely was a different experience, It was helpful... to their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science...

  39. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    ...Mentor – what do I do now? What are the benefits of taking on my own Mentee? Q1. What makes a good science...participants had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing...Mentor is often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela...on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do I Want To Be?” In this excellent essay...the northeast, writes a thoughtful treatise on the kind of advisor/mentor she wants to be for her new lab...

  40. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Anterograde transport is typically used for the trafficking of organelles, such as mitochondria, as well ... dynein, while anterograde transport relies on kinesin. Different rates of speed for various forms of ...particles will infect neurons at different ratios, making these neurons fluoresce in different colors. An... The underlying mechanisms of trans-synaptic trafficking are not fully understood and may be a form of...B.T., Maguire, C.A. and Ramirez S.H. (2017). Trafficking of adenoassociated virus vectors across a model...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa... Resources on the Addgene Blog Retrograde AAV: Making the journey from axon to nucleus Rabies and neuronal...

  41. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...Matt Takvorian When COVID-19 researchers started working with us to help them share their reagents, we knew...COVID-19 resources are now available to scientists working in a for-profit setting.  In light of the need ...and able to listen to Addgene’s new approach of working with the industry-based scientific community. Conversations...California Institute of Technology             Johns Hopkins University                           National Institute...from the Michael J. Fox Foundation are used in Parkinson’s research.  The extensive resource from the Structural...

  42. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...their careers to get advice on choosing a lab, picking your research topic, starting a lab, and more. ...skills that employers love PhD students to have: working to deadlines, writing up clear and attractive reports...reports, being good at presentations and of course, networking and collaborating.” Getting out of the lab also...out that a PhD teaches analytical and critical thinking skills that are applicable to a variety of careers...passionate about. Check out my previous post if you're looking for more information about the benefits of pursuing...

  43. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...self-excising cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression...2013), or rescue of mutant phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective...knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more efficient. Pure...these improvements from the Mello lab resulted in strikingly high editing efficiencies, obtaining as many ...e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein...

  44. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When...typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined a gym,... potential cures. We encourage any researchers working on ACVR1 or any of the pathways that impinge upon...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue ...structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His...

  45. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...about science to everyone – not just to others working in your own field. Alternative careers are now ...necessarily bad news, but it is an argument for taking the time to hone your communication skills. So,...ll know you are on the right track if they start asking you questions. Remember that people like a good...   Consider starting up your own twitter feed or talking about and sharing some science on the one you already...ocean science, they have good advice for anyone looking to make their science matter. If you aren’t ready...

  46. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...lab. A common strategy to determine the binding kinetics of a purified protein involves immobilization ...moiety, which forms dimers, may influence binding kinetics of the target ligand. Another important consideration...-glutathione. Biotin-based immobilization for kinetic analysis Proteolytic removal of the fusion protein...protein could provide a more relevant kinetic analysis, but the trick is to utilize a second immobilization...outlined here provides a basis to determine binding kinetics using a multitude of methods including surface...

  47. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...recombinant affinity reagents. Addgene is looking forward to working with the community to create a fully ...plasmid and viral services. The first step will be working with the community to build a resource of plasmids... well validated collection. I’m thrilled to be working with Addgene to bring this resource to life,” says...10.1038/520295b Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...

  48. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...researching, but I decided I needed a new challenge. Looking for sales and marketing jobs Like most choices ... researchers. Many biotechnology companies are looking for PhD or Master’s level scientists who have knowledge...business-like elements. At this point you may be asking yourself, "Why would you switch the focus of your...the employer was not such that he/she would be looking at where I had published, who I had published with...scientific and marketing knowledge. For most PhDs looking at careers outside of academia, sales and marketing...

  49. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ... accountability that is one major advantage of working with a mentor.The responsibility of arranging meetings...that need work or growth. Here are some ideas for making your meetings productive: Discuss logistics if...reach all the goals you set, but the process of working on them is where growth happens. Mentors bring ... day. If I skip, I get a bunch of text messages asking me, “What happened? Where are you?” My gym posse...Develop coaching skills Encourage your creative thinking Enhance leadership skills Support inclusion, diversity...

  50. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands on While...meeting with some other professors, I went home thinking about how to solve this problem. I didn’t want...your strategy. I realized that similar types of thinking go into developing gene therapy strategies for...noting any gaps in their knowledge. Practically speaking the format I decided on was to have two teams ...skills like team building, collaborative critical thinking, and communication among others. They additionally...
Showing: 301 - 350 of 929 results