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  1. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists... this alone. If you have deposited plasmids at Addgene and would like to make them available to industry... us know below! Additional resources on the Addgene blog Browse blog posts related to COVID-19 Learn...

  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet...looser PAM requirements, which are available from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from...CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence...Conclusion Ready for some more details? Check out the Addgene CRISPR guide or our CRISPR 101 ebook. We also ... Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

  3. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post
    ...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ... better way to search First, check out the new Addgene homepage. You may have noticed the search field...indicate that their plasmids are available from Addgene in the Methods & Materials or elsewhere in their...from the James Trimmer Lab published in eLife. Addgene is currently distributing 94 plasmids expressing...someone has already created and deposited it with Addgene. Combining search terms with filters lets you drill...to apply a wide range of filters isn’t new to Addgene’s plasmid search. The new design of the plasmid ...

  4. Call for Guest Bloggers

    Type
    Blog Post
    ... Sign up to write for the Addgene blog! To sign up to write for the Addgene blog simply email us. We can...write for us! Click Here to Contribute to the Addgene Blog Possible guest topics Some topics you might...Exposure - With over 100,000 views each month, the Addgene Blog is a great way to get your name out there!...include on CVs and Resumes. An Expanded Network - Addgene will promote your blog on a variety of social media...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post
    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Cas9 Activators: A Practical...specific genomic loci is possible using CRISPR, and Addgene has a number of tools for this purpose. Epigenetics...CRISPR-based epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead...epigenetic modifiers, and is available as a kit from Addgene.   Find Ready-to-Use Viral Preps for Epigenetic...nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song...nbt.3853 Find plasmids from this publication at Addgene.  Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang...ncomms16026 Find plasmids from this publication at Addgene. Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna...

  6. How Addgene Creates Content

    Type
    Blog Post
    ...everything else we do! The goal of Addgene’s educational content Addgene truly believes that sharing speeds...you ever wondered how we choose topics for the Addgene blog? Or why we make certain videos? In this post...You probably won’t be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs...topics. What better space to do that than in the Addgene blog?  Fig. 1: One of our favorite content...sure things are clear, accurate, and in line with Addgene style.        Fig. 2: Reference image (...already comes from you and the questions you ask Addgene and your research community! We work to provide...with your help.     Additional Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors...

  7. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... are members of the Addgene Advisory Board. Additional Resources on the Addgene Blog Get the Most from...plasmids for that particular organism available at Addgene. Percentages represent the fraction of that total...available to the academic research community. At Addgene, we’ve helped distribute many of these technologies...Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty of producing ...John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you're setting up to...Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's ability to ...anytime you order ready-to-use lentivirus from Addgene, you’ll also receive transfer plasmid DNA that ...

  8. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post
    ...versions of 15 different disease-associated genes. Addgene spoke to Musunuru about how the new kit works, ...and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with this new TALEN kit,...either knock out a gene or knock in a mutation. Addgene: How do you use this kit to target a particular...cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru: We ...one needs to do disease modeling in stem cells. Addgene: Tell me about the history. How had investigators...genetically modified cells in less than a month's time. Addgene: What are the implications of this kit in terms...becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN kit to CRISPR/Cas9? ...

  9. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    Blog Post
    ... biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors.../ protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs...restriction enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences...tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence manipulation... Rachel Leeson.  Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker...time and track your experiments  Resources on Addgene.org: Read our Molecular Biology Reference Find molecular...

  10. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...guest writers. Your contributions make Addgene and the Addgene Blog useful resources for the biological...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...highlighting some of the great posts written by Addgene depositors. Below you’ll find brief summaries of...depositors. Interested in writing your own post for the Addgene Blog? Fill out our guest blogger form to get started.... Note: Casilio is no longer available through Addgene.  Thanks again to all of our fantastic depositors... addgene...

  11. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post
    ...data Do you use Addgene AAV tools and want to join the ranks of our growing group of Addgene AAV data sharing... way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot in 2019 as... an open science platform for scientists using Addgene AAV viral preps to easily find and share useful...animal strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over...sharing champions? If you’ve recently used Addgene AAV tools, we invite you to contribute your data to ...data on AAV data hub! Related resources on the Addgene blog Important Considerations When Using AAVs ...Using AAV for neuronal tracing Resources on Addgene.org Read our AAV guide Browse all  AAV vectors Find...

  12. Running for Rare Disease, Running for FOP, Running for AJ

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    Blog Post
    ...FOP until a cure is available. Addgene and AJ's Avengers This year Addgene, where my wife Lianna has made...their plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers...helping us fight against FOP in two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...

  13. Addgene's Top 10 Blog Posts of 2015

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    Blog Post
    ...2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...have started providing recorded interviews with Addgene community members. In addition we’ve experienced...them a read if you’ve missed them! Breakdown of Addgene's top blog posts from 2015 Our most popular posts...plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications Intern Mary Gearing explains... need them. Happy Reading!   Resources on the Addgene Blog Download the Management for Scientists eBook...101, CRISPR 101, and Career Posts Resources at Addgene Browse Our CRISPR Resources Learn More about Viral... Addgene...

  14. Our New Antibody Mascot is....

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    Blog Post
    ... be in touch with you soon with some brand-new Addgene swag!   Last month, we introduced you to Blugene...Blugene and Aavery's newest friend, our Addgene antibody mascot, here to help us with our upcoming affinity...this form or head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022...that has led them to their biggest discovery yet: Addgene will soon be distributing affinity reagents. Name... four will receive some rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig....Submissions are open until Feb. 3, 2022.  The Addgenies will choose up to four finalists, and the final...

  15. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ... also find information on using Addgene’s website, the latest Addgene news, and awards that we offer there...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...articles you want to find without even leaving the Addgene blog! Just click on the search bar at the top of...Be the first to know about new articles on the Addgene blog by subscribing to instant alerts below.   ...author pages, where you can read more about the Addgenie behind the blog post and find more of their work...interview. Get in touch by emailing us at blog@addgene.org or tell us in the comments below. We’ll track...

  16. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning...the UK, South Korea, and beyond!   Check Out How Addgene Thinks About Developing Lab Management Software...plasmids and you decide to share them using Addgene’s repository, you can also share your associated protocols...gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular... to learn more about sharing your plasmids via Addgene? Check out all the benefits and how you can get...

  17. The time and cost required to make a plasmid

    Type
    Blog Post
    ...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...not an economist! Additional Resources on the Addgene Blog 5 factors to help you choose the right cloning...

  18. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post
    ...Resources at Addgene Find the ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out... al., 2016).  Bhang deposited the library with Addgene because she wanted to make it as easy as possible...and she is excited to deposit the library with Addgene because it is a “really great way of broadly sharing...sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative new ways you put ...and for providing this powerful resource to the Addgene community! Carrie Bhang is a research investigator...PMCID: PMC4900892. Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening...

  19. Congratulations, Deck The Lab winners!

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    Blog Post
    ...remotely instead of from the lab. Nonetheless, Addgene’s annual Deck the Lab competition drew upon the ...Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter.com/drNBUdlQco — The Tarling-Vallim...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr. Anne A Madden ...thank you to everyone who entered. Keep an eye on Addgene’s social media throughout the year for more fun ...decorations in the lab, and one for home. The Addgenies voted, and selected three winners in each category...

  20. What's New in CRISPR - Winter 2018

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    Blog Post
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...adapted for detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the...editing is now possible. Learn more from this Addgene blog post Read the paper in Nature Methods Find...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  21. Hot Plasmids: Spring 2025

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    Blog Post
    ...this year!  Find Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene cloned EGFP into...Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081), then generated...particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected...Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat...increased sensitivity and tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant...2025.03.20.643984.  Build your way: Explore Addgene's new empty backbones By Meghan Rego You asked, ...EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these new backbones special:...

  22. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post
    ...As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation... for NGS-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids...are using this technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell...does not vary by DNA input amount. plexWell™ + Addgene = high quality NGS-assembled plasmids Because of...original sample. At seqWell, we have worked with Addgene to develop a highly optimized bioinformatics pipeline...circularized plasmids from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped...NGS-based assemblies for over 3000 plasmids in the Addgene collection.   Check out the SeqWell website to...

  23. 25 Work From Home Tips for Scientists

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    Blog Post
    ...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub ... to use in future experiments. If you’ve used Addgene’s AAV tools, you can contribute data to the AAV ...

  24. Pooled Library Amplifications

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    Blog Post
    ...Libraries Resources on Addgene.org Addgene's Guide to Plasmid Pooled Libraries  Addgene's Pooled Library Collection...contact us at deposit@addgene.org. Happy amplifying! More resources on the Addgene blog Genome-Wide Screening...If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an ...recombination and loss of plasmid diversity, so Addgene strives to distribute aliquots of the original ...requesting scientist receives a pooled library from Addgene, they’ll need to amplify the sample for further...generally cheaper and easier. One library that Addgene distributes with a chemical transformation amplification...Table 1: Examples of Different Pooled Libraries at Addgene.  Library Type Name Number of Plasmids ...

  25. Participate in the Pets of Addgene Scavenger Hunt

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    Blog Post
    ...You will be trying to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each...just fun and games this April Fool’s Day on the Addgene blog.  We’ve been mostly working remotely for the.... Now, we’ve collected photos of our pets with Addgene gear, and have unleashed them all over our recently...

  26. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post
    ...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog  Read more about Cpf1... will the number of reagents available through Addgene! If you're trying to decide which CRISPR tools .../nature24049 Find plasmids from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim...science.aaq0180 Find plasmids from this paper at Addgene. Kim D, Kim J, Hur JK, Been KW, Yoon S, Kim J-...nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y,...ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, ...nature14592 Find plasmids from this publication at Addgene. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, ...

  27. Supporting Rare Disease Research with BeHeard 2017

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    Blog Post
    ...resources for those studying rare disease. This year, Addgene will be providing plasmid grants worth $2,000 each...BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate rare disease research...his work in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize smn1 interactions with...plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown, or overexpress these candidates...underrepresented individuals deserve a voice. Check back with Addgene often to learn of future rare disease research ...19 (2015): S64-S65. Additional Resources on the Addgene Blog Previous BeHeard Awardees Other Efforts to...Developing Countries with Seeding Labs Resources on Addgene.org Find Plasmids for Disease Research ...

  28. Introducing...Addgene Quick Tips!

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    Blog Post
    ...perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube! In this series...

  29. A Case Study of Addgene Bioinformatics

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    Blog Post
    ...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

  30. Introducing Addgene's Microbiology Pages

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    Blog Post
    ...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology.... coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters just ...microbial technology, sign up to write about it on the Addgene blog. ...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...organisms we should include by emailing us at help@addgene.org. If you are looking for plasmids used in E. ...

  31. 2022 and 2023: Reflections and Looking Forward

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    Blog Post
    ...quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...organization. One thing that will remain the same is Addgene’s commitment to supporting the research community...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger...

  32. Your Top Requested Plasmid in 2015!

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    Blog Post
    ...announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries...reagents available to the research community through Addgene and look forward to another great year of sharing...PubMed Central PMCID: PMC4486245. Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral Guide Pages Find ...

  33. New Videos: Addgene Lab Tips

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    Blog Post
    ...blog@addgene.org.  Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...decided to share some of our tips in our new series: Addgene Lab Tips! In each short episode of this series...Science: We Share Science Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol...

  34. Lab to Office Culture Shock

    Type
    Blog Post
    ...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...making this transition. In this installment of the Addgene career guide, we’ll think about what causes this...job candidate for a senior scientist position at Addgene, the skeptical applicant asked if she would partake...of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list of things...the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is always ...someone who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly...fix them, and take a look at these tips from the Addgene blog. You’ll have the opportunity to discover...

  35. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post
    ...Introduction to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the construction...that will be the first antibodies distributed by Addgene.  The plasmids used to generate these antibodies...sequence. These plasmids have been deposited with Addgene for distribution to the scientific community with...affinity reagent tools to include nanobodies. At Addgene, we are excited to be part of this initiative and...References and Resources Additional Resources on the Addgene blog:  Plasmid-Based Recombinant Antibodies  Plasmids...

  36. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post
    ... available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids from this collection in your...cerevisiae expression vectors are available at Addgene. A set of mammalian expression vectors containing...COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received Ginkgo’s first... our standard full plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids...screening and the use of high throughput platforms, Addgene and Ginkgo are striking a balance by modifying ...978-1-59745-181-9_23 Additional resources on the Addgene blog Read all of our COVID-19 blog posts Learn...

  37. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids... addgene podcast...synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology ...

  38. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post
    ... field. Resources at Addgene Browse Addgene's RNAi resources Browse Addgene's CRISPR resources Read ...countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne...conference invited me to attend and speak about Addgene’s experience helping scientists share plasmids, ... talk to so many scientists who were potential Addgene requestors and depositors. China is in one of the...conference was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking...

  39. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

  40. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...but browsing the 49 new preps available in the Addgene viral vector repository with a nice cup of hot ...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

  41. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post
    ... autophagy biosensors currently available from Addgene. Rosella autophagy biosensor Rosella is a dual-...-targeted and mitochondria-targeted Rosella at Addgene! Keima autophagy biosensor Keima is a mammalian...Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy... red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases... flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo...PMCID: PMC5618514. Additional resources on the Addgene blog Beware of fluorescent protein oligomerization... sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent...

  42. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Plasmid Rap Plasmid rap video made with support from Addgene.      Check out more science education tools...Additional Resources on the Addgene Blog Get Advice on How to Build a Grad Student Community Check out All...Plasmids 101 Series Additional Resources on Addgene.org Check out Our Protocol Pages for New Protocol...

  43. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing open source sharing...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...

  44. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook...end goal. CasPEDIA also features useful links to Addgene resources throughout the site. In the Experimental...each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that enzyme... from the article if they're available through Addgene. So whether you're looking for the exact Cas9 plasmid... or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered!     ... eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment...

  45. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's most popular retroviral...retroviral plasmids Addgene's collection of AAV plasmids Addgene’s collection of adenovirus plasmids Resources...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...components of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for ...Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral Packaging Plasmids...

  46. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post
    ...they grow. The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral...components over the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes...2A-Puro (PX459) V2.0 was made available through Addgene in 2015, making this the most recently available...fact: published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd...shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more...projects in the years to come.  Resources on the Addgene blog Browse our CRISPR posts Read our viral vector...posts Read our lentivirus guide Resources on the Addgene website Browse our CRISPR collection Find plasmids...

  47. Addgene's Tips for Plasmid Quality Control

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    Blog Post
    ... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence... plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we distribute...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...plasmid you are working with, but, in most cases at Addgene, we check what we call the “insert,” (the gene ...comments section below.  Additional Resources on the Addgene Blog Get 6 Tips for Analyzing and Troubleshooting...

  48. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post
    ...Marillonnet share with the scientific community via Addgene, the struggles of plant scientists and how they...Everybody's favorite parts for plant synthetic biology Addgene: Tell me about the Golden Gate Modular Cloning ...elements, which has not been available for plants. Addgene: Will the MoClo tools work in any plant? Patron... it a good laboratory workhorse. Tips & tricks Addgene: Do you have any particular tips for those looking...start and go ahead. I can see science speeding up. Addgene: Are there other tools you are working on for plants...interaction work and we’ve gotten some tools back from Addgene recently to explore their use in plants, including...technologies in the near future. Science on Twitter Addgene: You are active on twitter @nicolabiologist, where...

  49. Your Lentiviral Plasmid FAQs Answered

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    Blog Post
    ...about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview...lentiviruses can infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids...lentiviral questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono'...gritty and answer the questions those of us at Addgene get asked most often: Q1: Are retroviruses and ...and 3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors... competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are replication-deficient meaning...Trono Lab: Lentivectors Toolbox Resources on the Addgene Blog: Download our eBook: Plasmids 101 Learn about...

  50. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post
    ...Golden Gate Cloning Additional resources on addgene.org Addgene's MoClo Guide and Kits MoClo Toolkit MoClo...chosen one of the MoClo kits available through Addgene that suits your experimental purposes. But what...park.  Before you begin, be sure to check out Addgene’s MoClo guide for all the basics of the process....coding sequence of interestCDS Level 0 part from an Addgene MoClo kit, to construct a Level 1 vector with a...the plasmid sequence files provided with every Addgene MoClo kit through their online catalog pages. We...your MoClo kit has just arrived! MoClo kits from Addgene will most likely arrive in your laboratory as plates...recommend retrieving plasmids from your original Addgene kit plate as few times as possible and always under...
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