We narrowed to 274 results for: AAV
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TypeBlog Post...details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch... switch to droplet digital PCR to quantify AAV titers. Get the details on the methods in this blog post...
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Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...cells, commonly through viral delivery by AAV. You can find AAV tools for optogenetics and chemogenetics... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...diseases. The researchers injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a...Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal...viral service Find viral protocols and videos Find AAVs for your research ...vigilance. A recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor...specifically activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver... -
Beginner's Guide to Viral Vectors
TypeBlog Post...important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide. Print... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post... viral service Find optogenetics AAVs for your research Find all AAVs from the viral service ... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...are named after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And... Vector Elements More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...producing your own vectors in the lab to optimizing AAVs in your experiment. Our second edition includes... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post... it can be packaged into adeno-associated virus (AAV). At 984 amino acids in length, Cas9 from Campylobacter... smaller than SaCas9 and is also compatible with AAV delivery. NmCas9, another small ortholog, displays... Read more about Cpf1 Read more about SaCas9 and AAV Check out our CRISPR Featured Topic Page Resources... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and 5HT sensors New AAV tools to selectively...from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps ... -
15 Hot Plasmids from 2017
TypeBlog Post...interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict ...to this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox ...these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally manipulate...tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here...27798629 Listen to Our Podcast Segment on This New AAV Tool mCyRFP1: Novel fluorescent protein that... -
How Addgene Creates Content
TypeBlog Post...Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website, Data Hub...enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists to easily... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...then easy to package in low-capacity vectors like AAV, a popular vector due to its low immunogenicity. ...acceptor and two putative splice enhancers. After AAV-mediated delivery to iPS-derived cortical neurons... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...and shared the paper with my lab since one of the AAV vectors in the paper seemed like it could help one...my lab mate’s with her project. Unfortunately the AAV didn’t give us the results we hoped for, but it did... -
Plasmids 101: Cre-lox
TypeBlog Post...Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce... -
Hot Plasmids: Summer 2024
TypeBlog Post...proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed targets. ...from Neumann et al. 2024. The team showed that AAV-delivered CHARM could silence the expression of prion... (2024). Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing...facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline modification...especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important...with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...the protein of interest. In our studies, we used AAV viral vectors to express hM4Di in neurons of rhesus...years ago (Field et al., 1988). Thus, we injected AAVs carrying hM4Di-HA in the brains of mice and monkeys... -
Addgene: The First Twenty Years
TypeBlog Post...vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing supportive...be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...and 100% of DsRed+ cells also expressed GFP. Using AAV constructs, Tang et al. found that Cre-DOG could ...optogenetic studies, and since cells infected with AAV-Cre-DOG retain normal neuronal function, this system... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids deposited by Ian...