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We narrowed to 54 results for: AAV

Showing: 1 - 20 of 54 results

  1. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...HEK293 cells) during AAV production. Read our AAV Guide for more information about: AAV components Common...AAV8 AAV packaging plasmid, expressing Rep2 and Cap8 James M. Wilson 112865 pAAV2/9n AAV9 AAV packaging ...rh10 capsid James M. Wilson 81070 rAAV2-retro helper AAV retrograde AAV packaging plasmid, expressing Rep2... Virus (AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids...Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids Adeno-associated...immunological effects in vivo . While AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should...to the reliance of the AAV on certain exogenous gene products that mediate AAV replication. This requirement...

  2. Brain Armamentarium

    Type
    Collection
    ...Alias: pAAV_WDP0003_GCaMP6f Gordon Fishell Viviana Gradinaru 213941-AAV1 pAAV_BiPVe3_ChR2_mCherry AAV construct...Alias: pAAV_WDC0004_GCaMP6f Gordon Fishell Viviana Gradinaru 213937-AAV1 pAAV_BiPVe4_ChR2_mCherry AAV construct...Alias: pAAV_WDL0003_jGCaMP8m Gordon Fishell James M. Wilson 213917-AAV1 pAAV_BiLAMP5e3_GCaMP6f AAV construct...Alias: pAAV_YWE4.2_GCaMP6f Gordon Fishell Viviana Gradinaru 213856-AAV1 pAAV_BiVIPe4_ChR2_mCherry AAV construct...Alias: pAAV_S9E10_GCaMP6f Gordon Fishell Viviana Gradinaru 213815-AAV1 pAAV_BiSSTe10_ChR2_mCherry AAV construct...Science , Bosiljka Tasic Melina Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier...enhancer. Alias: pAAV_WDC0004_eGFP Gordon Fishell James M. Wilson 221463-PHPeB pAAV_BiPVe4_eGFP AAV construct...

  3. Retrograde AAV viral preps

    Type
    Collection
    ...Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part of our Viral...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Hongkui Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA...Project 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Douglas Kim 104489 pGP-AAV-syn-jGCaMP7b-...Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f Calcium sensor Douglas Kim 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE...Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE Project 162375 pGP-AAV-syn-jGCaMP8m-WPRE...

  4. Recombinases AAV Preps

    Type
    Collection
    ...Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps that encode site-specific...Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore...with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40...William Pu 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB James M. Wilson 196410 AAV-GfaABC1D-Cre-4x6T...

  5. Brain Initiative Collection

    Type
    Collection
    ...vectors. Jonathan Ting 172907-AAV1 pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor...GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven...enhancer element Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1...of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression...

  6. Biosensor AAV Preps

    Type
    Collection
    ...Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our AAV that express ...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg* GENIE 167572 AAV-hSyn-Ribo-jGCaMP8s...176752 AAV-CamKIIa-jGCaMP8s-WPRE CamKIIa jGCaMP8s none Constitutive 1, 5, 9, rg* Looger 176755 AAV-mDlx-...

  7. Serotype Testing AAV

    Type
    Collection
    ...Service AAV Serotype Testing AAV Viral Vector Packaging Service: Serotype Testing AAV These AAV encode ...for serotype testing: AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, and AAV retrograde. Viral...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list of ...Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid... Cart AAV9 37825-AAV9.T 20 µL $ 150 Add to Cart AAV11 37825-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde...150 Add to Cart AAV11 50465-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde 50465-AAVrg.T 20 µL $ 150 Add...have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description...

  8. Caltech Systemic Capsids

    Type
    Collection
    ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP....and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-...GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE...scientific community. Text Description for the AAV Decision Tree The AAV decision tree graphic above helps you ...

  9. AAV Viral Preps

    Type
    Collection
    ...Vector Packaging Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful...Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech... in one of seven serotypes (AAV1, AAV2, AAV5, AAV6, AAV8, AAV11, or AAVrg). For more information, visit...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro...protection levels. High quality and high titer in-stock AAV preps are affordable, available in small volumes,...

  10. Viral Production

    Type
    Collection
    ... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral...Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at ... # 84445-AAVrg). Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles...production methods and quality control for lentivirus and AAV. Viral...Viral Production Jump To: Viral Production Overview AAV Production Titer Quality Control Resources Lentivirus...

  11. AAV Molecular Tools

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and ... Gradinaru Tools for Affinity Purification These AAV encode tools for affinity purification (which can...Nectow , Eric Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology...Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...mapping 5 Jinhyun Kim Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ...

  12. AAV Packaged on Request

    Type
    Collection
    ...using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep ...can be ordered in seven serotypes: AAV1 AAV2 AAV5 AAV6 AAV8 AAV11 AAVrg Want more information before choosing...Discover our AAV Packaged on Request service with all-in-one pricing for viral vector preps, which includes...Viral Vector Packaging Service Packaged on Request AAV Packaged on Request Overview Details Ordering Share...Plasmids Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools ...effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro...choosing a serotype? Learn more about AAV serotypes and tissue tropism on Addgene's blog . All-in-One Pricing...

  13. AAV for Neuronal Tracing

    Type
    Collection
    ... Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA...deletion-mutant rabies virus AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be ...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging...to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all the viral genes...

  14. Chemogenetics AAV Preps

    Type
    Collection
    ...Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics AAV Use our chemogenetics AAV to chemically... labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See What You’re Looking...Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor...fewer filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral... fusion Flp-dependent 8, rg* Ulrik Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition...IRES EGFP Cre-dependent 5, 9 Scott Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition ...

  15. Control AAV Preps

    Type
    Collection
    ...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express...options. Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including...Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre dependent 9 Joshua Sanes 45186 AAV-EF1a-BbChT EF1a...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8...AAV8 AAV9 AAVrh10 AAV11 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter...Roth 51506 AAV phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Hongkui Zeng 58909 pAAV-GFAP104-mCherry...

  16. Viral Vector Guides and Plasmids

    Type
    Collection
    ...into the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly...on Request service for made-to-order AAV vectors from Addgene's AAV plasmid collection. Addgene also has... generally don't integrate into the host genome. AAVs are limited by their small packaging capacity. Gamma-retroviral...suited for specific research goals. For example, AAV vectors are typically preferred for in vivo studies...Stable RNA < 8 kb 80–130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼...105 nm Dividing/Non-dividing No High *Recombinant AAV has a low frequency of target cell genome integration...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ...Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly... lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin...#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA...This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR... Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab has both single...Cas9 and sgRNA in vivo via adeno-associated virus (AAV). The single vector system uses a smaller Cas9 from...

  18. Optogenetics AAV Preps

    Type
    Collection
    ...Browse all Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, ...Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic tools ...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell...28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Karel Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...Boyden 62726 pAAV-Syn-Chronos-tdTomato Syn Chronos tdTomato Constitutive 8 Edward Boyden 130909 AAV-CAG-FLPX-rc...Deisseroth 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Edward Boyden 28305 pAAV-FLEX-ArchT-tdTomato...

  19. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...You may also like: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida... Viral Vector Packaging Service AAV University of Florida Serotype Testing Panel for ...viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) ...transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71...One . Jun 8;10(6). PMID: 26052939 Lu, et al. 2016. AAV-mediated transduction and targeting of retinal bipolar...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma and their...

  20. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...viral vector technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been...Viral preparation options include lentivirus and AAV. Viral...ready to ship immediately after MTA approval. Browse AAV Browse Lentivirus Packaged on Request Choose from...reports from the research community demonstrating AAV performance under various experimental conditions...
Showing: 1 - 20 of 54 results