Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,089 results for: ANT

Showing: 121 - 140 of 1089 results

  1. p53 Pathway

    Type
    Collection
    ...PubMed PMID: 24379683 . When mutants gain new powers: news from the mutant p53 field. Brosh R, Rotter V...to activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization... of p53’s pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional...-of-function, expanding the mechanisms by which mutant p53 is known to function in cancer. p53 Pathway...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...2003 Mar;21(3):313-20. PubMed PMID: 12619118 . p53 mutant mice that display early ageing-associated phenotypes...of p300/CBP in p53-dependent signal pathways. Avantaggiati ML, Ogryzko V, Gardner K, Giordano A, Levine...

  2. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...first set of anti-CRISPR genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR genes ...Cpf1) Type IV (Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive immune...complex. Recently, researchers have begun to use anti-CRISPRs as off-switches for CRISPR genomic editing...libraries can be used to identify genes that are important to a given phenotype. Current libraries are available...editing rates. Advances in this area include: Nickase mutant: cuts only one strand of DNA and can be used with... High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very...to be used together. Researchers have engineered mutant Cas enzymes with alternative PAM sites . Cpf1 ,...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...: SepRS adds a phosphoserine to tRNA^Sep with anticodon, CUA. Engineered EF-Tu binds the phosphoserine-charged...Phosphorylation Protein phosphorylation is one of the most abundant forms of posttranslational modification in cells...University changed the way researchers can explore important questions surrounding serine phosphorylation by...overall improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli... of the popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus...phosphosite library (Pooled Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic...reference spectra or phosphopeptide standards for quantitative assessments. The Mode #1 phosphosite library...

  4. Rett Syndrome

    Type
    Collection
    ...Tissue Bank Antibodies (Link opens in a new window) N-terminal Anti-MECP2 (M7443) mouse antibody from Sigma...C-terminal Anti-MECP2 (M6818) mouse antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2 ... expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these X inactivation ratios ...syndrome at the molecular level. They are also an important tool for developing therapeutic strategies to ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

  5. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair..., RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. In... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources... This technique creates diverse populations of mutants for directed evolution. ID Plasmid Gene/Insert ... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands...

  6. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair..., RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands...

  7. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... protein component of MMR) mutant to inhibit MMR PE5 — PE3 plus MLH1 mutant to inhibit MMR PEmax — optimized...QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao 141081 pYPQ141D-peg Plant OsU3 pegRNA ...Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources Prime editing is a “search... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3 pegRNA BsaI + HindIII No Caixia Gao 172716 ...

  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair..., RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands...

  9. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3)...Mutated Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient Transduction of Trabecular...Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant AAV2 vectors in the mouse retina. Mol Ther . Feb...mesenchymal stem cells into pluripotent stem cells by a mutant adeno-associated viral vector. Hum Gene Ther Methods...in Cynomolgus Macaques of rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin...Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol . Mar... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  10. Fluorescent Proteins: FRET

    Type
    Collection
    ...maximum excitation wavelength (nm), QY D : Donor quantum yield, λ Aem : Acceptor maximum emission wavelength...exctinction coefficient (M -1 cm -1 ), QY A : Acceptor quantum yield, R 0 : Förster Radius (nm, distance at which...linkers of varying length Evers et al. (2006) Quantitative understanding of the energy transfer between...FRET efficiency standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for...stoichiometry of protein interactions using image-based quantitative two-hybrid FRET Positive and negative controls...evolution of cyan fluorescent proteins towards a quantum yield of 93% Positive and negative controls and...FRET pairs Gadella et al. (2023) mScarlet3: a brilliant and fast-maturing red fluorescent protein Variety...

  11. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Mammalian Bacteria Drosophila Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand...Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair...created by wild-type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

  12. Cancer Research Plasmids and Resources

    Type
    Collection
    ...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types... for use in cancer signaling studies, including mutant, knockdown, and overexpression constructs. Each...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...location, their pathway dependencies are extremely important for classification and treatment. On each pathway...Meyerson , and Root Labs containing wild-type and mutant alleles described in the initial Target Accelerator...

  13. Genetic Code Expansion

    Type
    Collection
    ...ribosome and if the anticodon on the tRNA can bind to the mRNA (hence, the anticodon is complementary to... by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA variant inactivated... genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal...aaRS M. jannaschii sulfotyrosine (sY) Bacterial, Plant TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine...inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS/tRNA pair instead. For directed...knocked out, tyrTV and tyrU genes replaced by tRNA variant that suppress the TAG nonsense codon). Expresses...

  14. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL for the AAV1... you know that we don’t expect to produce our guaranteed titer, and will give you the option to proceed...proceed with your order, or not, without a guaranteed titer. Any titer expectations communicated by email ...email will supersede the general guarantees above. We will have our expert viral vector production team carefully...our viral production does not yield the titer guaranteed here or by email, Addgene will contact you to...carried out with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs...

  15. Synthetic Biology - Overview

    Type
    Collection
    ... Standard European Vector Architecture Open Plant - Plant SynBio resource Synthetic Biology - Open access...Mukhopadhyay Richard Murray Erin O'Shea Martin Parniske Antonio Richart Herbert Sauro Claudia Schmidt-Dannert Pamela...pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio...FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit ...Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway...

  16. AAV for Neuronal Tracing

    Type
    Collection
    ...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2...the cell population of interest with a deletion-mutant tracing virus that lacks the ability to infect ... To enable monosynaptic spread of this deletion-mutant rabies, the essential G protein is provided to ...2007a. Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  17. Bacterial Expression Systems

    Type
    Collection
    ... to enhance protein solubility and to direct recombinant proteins to the periplasmic space between the...bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium Modular Assembly Kits...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...leads to slow bacterial growth, or maybe you only want to study the effects of protein expression in the...the stationary phase. In these cases, you may want to control protein levels or to turn on expression ...presence of that small molecule and sometimes even quantify it. Bacterial one-hybrid (B1H) systems also use... or introducing specific mutations can reveal important insights into gene function. These genetic changes...

  18. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination events...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...turning on another. The FLEx switch system takes advantage of the orientation specificity of Cre and the ...different types of target sites available, both mutant and wild type. The ability to manipulate the number... to genomic instability and cell death. It is important to incorporate appropriate controls such as Cre-only...

  19. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...CRISPR systems, the most abundant type found in bacteria and archaea. While abundant in nature, Class 1 systems...CRISPR Plasmids: Cascade-Cas3 Mammalian Bacteria Plant Resources Cas3 is a unique member of the CRISPR ...double-strand break, either due to a boundary defined by anti-CRISPR proteins or running into other proteins bound...Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter PI Publication Return...

  20. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Edit CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors... base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements...fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or ...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...
Showing: 121 - 140 of 1089 results