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  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...(HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et al., 2023. nLight nLight...Kiss, C., Curreli, S., Dieter, A., Wilhelm, M., Harada, M., Duss, S. N., Dernic, J., Bhat, M. A., Zhou...

  2. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ..., the ability to embrace serendipity fosters adaptability, a crucial skill for a successful research career...research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries...

  3. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...antibody-dependent cellular phagocytosis (ADCP). Figure adapted from van Erp, et al., 2019.    Antibody-dependent...Song, WC. Complement and its role in innate and adaptive immune responses. Cell Res 20, 34–50 (2010). https...

  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor multiple ...- Overview of the neural and gut fiber system (adapted from Sahasrabudhe et al., 2023).   To develop...

  5. What the HEK?

    Type
    Blog Post
    ...adherent monolayer, though derivatives have been adapted for growth in suspension. Of the many derivatives... allows for large scalability, and can also be adapted for serum-free growth, which significantly reduces...

  6. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...tool for enrichment of ABE-targeted cells By Ellie Adams Single base modifications through ABE or CBE's have... from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian cell lines. ...

  7. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...recombinase-based state machines and how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-... understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert...

  9. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances,...mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed...

  11. Hot Plasmids - January 2023

    Type
    Blog Post
    ...nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022.   Find CRISPR...temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods ...

  12. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Arabia -- as well as closer to home in the US and Canada. In fact, we received so many requests (175 and...imagine, it’s also been quite the administrative headache receiving constant email requests and tracking...

  13. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule... your work schedule as possible so that you can adapt to your kids' needs. Plan ahead. Think about and...

  17. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current... BioBuilder book chapter and an illustrated, downloadable classroom poster — with both of these resources...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...intra-molecular annealing. Figure and legend from Okada, 2013.   scAAV If your gene of interest is truly...PMID: 20649475. PubMed Central PMCID: PMC2957237. Okada, T. (2013). Efficient AAV Vector Production System...
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