We narrowed to 372 results for: BRE

...TEV-HsFbxo7-129-398/Skp1 FBXO8 GST, TEV tac Parkinson's Brenda Schulman 203365 Tau BA 12->7 K257T MAPT Flag CMV...Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's...

...yeast research, a few of those have been trips to breweries who happily exchange our expertise with lots of...the lab, it is important to take the occasional breather and acknowledge the team’s personal and professional...

... specific base pair edits without double-strand breaks Base editor (dCas9 or Cas9 nickase fusions with... a homology template, a large percentage of DNA breaks will be repaired by NHEJ instead of incorporating...

...through normal mechanisms. It will take the full breadth of our research expertise, but we believe that ...health for this current COVID-19 pandemic, and outbreaks that have yet to come,” said the team from the...

...this system, Cas9 generates a double-stranded DNA break at the genomic loci of interest and on either side...complementary to either side of the double-stranded break in the genomic loci of interest. As the universal...

...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase...

...searching the term "breast cancer" returns 40 screens performed in 26 different breast cancer cell lines...

...Conveniently, the process doesn’t cause double-stranded breaks and can deliver large sequences and even entire...allowed certain mutated phages to outcompete their brethren, and the researchers isolated these more efficient...

...stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the cleaved... of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning: 1. Create...

...research studies highlight the importance of taking a break and incorporating EAs into your routine.  Getting...learn new skills, while also getting a much-needed break from your lab bench.  Want further information ...

...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field. It has the possibility...7). An independent team led by Hans Clevers at Hubrecht Institute in The Netherlands used the CRISPR/Cas9...

...it’s acceptable if it’s in the name of beer and bread. The following table lists some of the biological...end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR)....

... during the day is a great way to take a mental break, while meditating right before bed will help you...it. Plan defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique...

...roles, including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...

...important biological information, amino acid abbreviations, and structures into one convenient resource...say that I still have the single and triple abbreviations of the amino acids memorized, but the structures...

... biosensor with two components - an enzyme that breaks down the HMO of interest into its component simple...and a lac operon controlled GFP. When the enzyme breaks down the HMO of interest, the lactose released ...

... Cleave target DNA resulting in a double-strand break (DSB) Cas9 and its variants have two endonuclease... which is E) cleaved leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the...

...principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant...exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo and in...

...unedited site distal tothe predicted double strand break, and a NHEJ/ “drop-off” probe designed to bind directly... directly at the predicted double strand break site (Figure 2A). The single molecule resolution of digital...

...and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...