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We narrowed to 376 results for: BRE

Showing: 121 - 140 of 376 results
  1. From our Table to Yours: An Inside Look at Lunch at Addgene

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    Blog Post
    ...accomplishing it all. How do we encourage people to take a break from their everyday tasks and make time for special...However, I think it’s just as important to take a break from work to share time with the people around us...
  2. Celebrating Accomplishments in the Lab

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    Blog Post
    ...yeast research, a few of those have been trips to breweries who happily exchange our expertise with lots of...the lab, it is important to take the occasional breather and acknowledge the team’s personal and professional...
  3. What's New in CRISPR - September 2019

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    Blog Post
    ...this system, Cas9 generates a double-stranded DNA break at the genomic loci of interest and on either side...complementary to either side of the double-stranded break in the genomic loci of interest. As the universal...
  4. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase...
  5. CASTing Off for New Shores in Human Genome Editing

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    Blog Post
    ...Conveniently, the process doesn’t cause double-stranded breaks and can deliver large sequences and even entire...allowed certain mutated phages to outcompete their brethren, and the researchers isolated these more efficient...
  6. Plasmids 101: TOPO Cloning

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    Blog Post
    ...stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the cleaved... of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning: 1. Create...
  7. Extracurricular Activities for a Strong Science Career Path

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    Blog Post
    ...research studies highlight the importance of taking a break and incorporating EAs into your routine.  Getting...learn new skills, while also getting a much-needed break from your lab bench.  Want further information ...
  8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field. It has the possibility...7). An independent team led by Hans Clevers at Hubrecht Institute in The Netherlands used the CRISPR/Cas9...
  9. 25 Work From Home Tips for Scientists

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    Blog Post
    ... during the day is a great way to take a mental break, while meditating right before bed will help you...it. Plan defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique...
  10. K. phaffii: Rising to the Occasion

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    Blog Post
    ...it’s acceptable if it’s in the name of beer and bread. The following table lists some of the biological...end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR)....
  11. Science Career Options

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    Blog Post
    ...roles, including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...
  12. 5 Great Apps for Lab Life

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    Blog Post
    ...important biological information, amino acid abbreviations, and structures into one convenient resource...say that I still have the single and triple abbreviations of the amino acids memorized, but the structures...
  13. Components of CRISPR/Cas9

    Type
    Blog Post
    ... Cleave target DNA resulting in a double-strand break (DSB) Cas9 and its variants have two endonuclease... which is E) cleaved leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the...
  14. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled receptor" into ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...
  15. A Deep Dive into BioBuilder

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    Blog Post
    ...and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...
  16. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ...principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant...exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo and in...
Showing: 121 - 140 of 376 results