... the importance of leaving room for serendipity during your PhD studies and offer two simple steps to ...capture fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling...

...brain slices Visualization of DA release dynamics during locomotion in freely moving mice Record DA dynamics... in live mice Report modulation of DA signaling during learning and reward consumption in live mice Find...

...technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to stabilize...transcriptional regulatory elements is controlled during development using the Drosophila wing as a model...

... project. Dissemination of the knowledge gained during this period occurs infrequently and rarely makes... wondered how we could make the insights gained during this time more visible and thereby have a positive...

...enterprise or that there weren’t some pretty hefty bumps during my graduate training. I particularly think that...that I thought about careers outside of academia during my graduate training. PhDs have many roles to play...

...substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...t new to Addgene, we are excited to share the procedures and Python scripts with laboratories and viral...

...different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF...

... Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth phase, but not the production phase,...release of functional vector into the culture medium during adeno-associated virus manufacturing." Human gene...

... planning, visualizing, and documenting their procedures. Preventable errors in the design of cloning ... powered plasmid maps Learn how we use SnapGene during the plasmid quality control process Check out full...

...mentor or anyone else available to directly help you during your visit. Reach out to your bench mentor (if ...and rights for anything that could be generated during the visit. If you don’t feel comfortable initiating...

..., their interactions and information processing during this condition is crucial. Plasmids expressing ...interesting combinations: biomedicine and astrobiology. During the last 11 years he has studied various aspects...

...translation is key to cellular function, especially during development or stress. With ribosome profiling,...double labeled, indicating translational repression. During later stages, single RFP-labeled osk and Oskar ...

...to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When... He first heard about RNAi at his MIT interview during a lecture given by Phil Sharp. After a few rotations...

...job. How can you incorporate the skills developed during graduate school into your resume? How can you stand...transferable skills? See Developing Transferable Skills During Science Training and So You Think You have Skills...

...protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a form of oxidation (...physiologically relevant exposure in intensity and/or duration. Both of these strategies carry the risk of off-target... contain the ccdB cassette, which is not copied during the PCR amplification step, so that only vectors...

...these omissions, so it is unlikely to be caught during the peer review process, which often focuses more... a particular result. Anything else that occurs during the course of the study, say a fifth time has unpredictably...

... club of its kind, running from start to finish during the academic year and remaining affordable for ...BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist mentors...

...open-source! Impressions from the poster sessions During the poster session I got a chance to talk with ...Visualizing dynamic microvillar search and stabilization during ligand detection by T cells." Science 356.6338 ...

...mismatched basepairs or small indels that occur during DNA synthesis, and protomoting or repressing protein...DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired mutation...

...Sample6, for advice on how to take an idea developed during graduate school and turn it into a biotech company...co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...