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We narrowed to 445 results for: DUR

Showing: 121 - 140 of 445 results
  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF...
  2. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...translation is key to cellular function, especially during development or stress. With ribosome profiling,...double labeled, indicating translational repression. During later stages, single RFP-labeled osk and Oskar ...
  3. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...mentor or anyone else available to directly help you during your visit. Reach out to your bench mentor (if ...and rights for anything that could be generated during the visit. If you don’t feel comfortable initiating...
  4. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When... He first heard about RNAi at his MIT interview during a lecture given by Phil Sharp. After a few rotations...
  5. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...job. How can you incorporate the skills developed during graduate school into your resume? How can you stand...transferable skills? See Developing Transferable Skills During Science Training and So You Think You have Skills...
  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a form of oxidation (...physiologically relevant exposure in intensity and/or duration. Both of these strategies carry the risk of off-target... contain the ccdB cassette, which is not copied during the PCR amplification step, so that only vectors...
  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... club of its kind, running from start to finish during the academic year and remaining affordable for ...BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist mentors...
  8. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...an issue as they are thought to be self-repaired during the AAV replication process (Chen et al., 2004)... ITR integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth...
  9. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...the body. Because calcium levels change rapidly during neuronal activity, scientists can use GECIs to ...shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater brightness, Less signal...
  10. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...mismatched basepairs or small indels that occur during DNA synthesis, and protomoting or repressing protein...DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired mutation...
  11. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case...
  12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic protein...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...
Showing: 121 - 140 of 445 results