We narrowed to 443 results for: DUR
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TypeBlog Post... project. Dissemination of the knowledge gained during this period occurs infrequently and rarely makes... wondered how we could make the insights gained during this time more visible and thereby have a positive...
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Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post...enterprise or that there weren’t some pretty hefty bumps during my graduate training. I particularly think that...that I thought about careers outside of academia during my graduate training. PhDs have many roles to play... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...t new to Addgene, we are excited to share the procedures and Python scripts with laboratories and viral... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post... planning, visualizing, and documenting their procedures. Preventable errors in the design of cloning ... powered plasmid maps Learn how we use SnapGene during the plasmid quality control process Check out full... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post... Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth phase, but not the production phase,...release of functional vector into the culture medium during adeno-associated virus manufacturing." Human gene... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...mentor or anyone else available to directly help you during your visit. Reach out to your bench mentor (if ...and rights for anything that could be generated during the visit. If you don’t feel comfortable initiating... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post..., their interactions and information processing during this condition is crucial. Plasmids expressing ...interesting combinations: biomedicine and astrobiology. During the last 11 years he has studied various aspects... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...translation is key to cellular function, especially during development or stress. With ribosome profiling,...double labeled, indicating translational repression. During later stages, single RFP-labeled osk and Oskar ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When... He first heard about RNAi at his MIT interview during a lecture given by Phil Sharp. After a few rotations... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...job. How can you incorporate the skills developed during graduate school into your resume? How can you stand...transferable skills? See Developing Transferable Skills During Science Training and So You Think You have Skills... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a form of oxidation (...physiologically relevant exposure in intensity and/or duration. Both of these strategies carry the risk of off-target... contain the ccdB cassette, which is not copied during the PCR amplification step, so that only vectors... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...these omissions, so it is unlikely to be caught during the peer review process, which often focuses more... a particular result. Anything else that occurs during the course of the study, say a fifth time has unpredictably... -
A Deep Dive into BioBuilder
TypeBlog Post... club of its kind, running from start to finish during the academic year and remaining affordable for ...BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist mentors... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...open-source! Impressions from the poster sessions During the poster session I got a chance to talk with ...Visualizing dynamic microvillar search and stabilization during ligand detection by T cells." Science 356.6338 ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...mismatched basepairs or small indels that occur during DNA synthesis, and protomoting or repressing protein...DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired mutation... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...Sample6, for advice on how to take an idea developed during graduate school and turn it into a biotech company...co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...general areas of interest and what you want to cover during the year. Suggest that each person bring 3 topics...preparation for the meeting or to be read or watched during the meeting. Scientists do better with process... -
All in a Twist: dsRNA
TypeBlog Post...stranded structure produced is known as an R-loop. During transcription, RNA is synthesized from a DNA template...DNA hybrids Persistent RNA:DNA hybrids that arise during transcription are typically recognized by RNase...