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We narrowed to 753 results for: ELP

Showing: 121 - 140 of 753 results

  1. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...listed? Help us serve you! Let us know about other organisms we should include by emailing us at help@addgene.org...editing techniques in your microbe of choice. For more help with CRISPR, read our recent blog post explaining...narrow your keyword search to one of these organisms. Help us continue to develop resources for your community...

  2. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...scientific community for years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast...constrained funding. Addgene is uniquely positioned to help research institutions maximize their increasingly...protocols remain freely accessible to all researchers, helping teams to be successful in their work despite limited...the scientific community. Let us know by emailing help@addgene.org. We look forward to hearing from you...

  3. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...some time thinking about which type of gradient can help answer your particular question before running one...Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel issues usually come down... cold room or packing the box with ice packs can help keep the system cool, as can lower voltages.  If...buffer around, which works well and consistently, to help troubleshoot background issues. It’s a quick way...run your western, check out our handy Table 1 for help in deciding which troubleshooting steps to take....* Load less sample/lysate  We hope this helps you on your western blot journey. Best of luck and...

  4. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...called our customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! ... order.  Still have questions? Visit the Addgene Help Center If you have additional questions about our...process not addressed in this article, check out our Help Center article How do I pay by purchase order (PO...questions about the ordering process, the Addgene Help Center is full of information about how to deposit...

  5. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...experience, It was helpful to have Addgenies reassure me that someone would always be there to help me. As the...Addgene’s Marketing Intern this summer! With the help of Project Onramp, I was able to gain access to ...down the right path. The frequent Zoom meetings helped me communicate my ideas. I was able to express ...

  6. Reflections and Looking Ahead

    Type
    Blog Post
    ...community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration. .... Building tomorrow’s science today Your voices helped shape our journey throughout the year. Through ...countless conversations both online and in-person, you helped us understand how Addgene can better serve the ...excited to continue to grow and innovate with your help and guidance. Every plasmid shared, every collaboration...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...and viruses, from crossing into the brain. While helpful from a biological standpoint, it makes delivering...information about commonly used and emerging capsids to help you decide which ones may be suitable for your experiment...area of interest, and health. While it’s always helpful to start with a literature search to see what others... promoters and enhancers (Mich et al., 2021) can help restrict expression to your cell type of interest...just reduces, empty viral capsids in your prep can help reduce the risk of an immune response. Strain-specific...table.     Figure 2: This decision tree can help guide your choice on which systemic capsid to use...options for systemic capsids, but we hope this post helps you find the right one to test in your application...

  8. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...there's a chance it'll never work. If you help others out, they'll help you too; both with your research and... not a fluke.” 3. Engage in various projects and help colleagues with their work. “Your one project may...with this passion. “Love what you are doing!” It helps when the time comes to work with full force. George...

  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...genome engineering, and studying its structure can help us understand the parameters of CRISPR experimental...arginine-rich bridge helix connects the two lobes and helps recognize the guide RNA. Unlike the cleavage domains...the CTD, ready to check for PAM sequencines. This helps explain why Cas9 can’t locate PAM sites until after...correctly before HNH or RuvC can cleave DNA, which helps limit off-target activity. What next? It’s not actually...different uses. In all cases, structural biology can help you visualize the system you’re working with and...

  10. Fostering communication in remote collaborations

    Type
    Blog Post
    ...techniques and tools that helped make my experience run smoothly and can help other teams successfully ...results or ask questions to your collaborators. This helps everyone feel included in the project and accelerates...members of your lab that you see in person, and helps improve your interpersonal relationships with people...

  11. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...like to help share useful new resources with the scientific community – particularly ones that help address...lines you are currently or planning to use for helpful information on the known features and relevant ...reference, and shared allele search preferences to help you identify proximal lines you care about. If you...

  12. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...now has a helpful graphic to introduce you to the plasmid class covered on that page. To help you find ...also give you high-quality educational resources to help you do better research. CRISPR is an incredibly ...research toolbox, as well as additional resources to help you Plan Your Experiment. #12DaysofCRISPR and blog...

  13. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions...you requested from Addgene...the plasmid that will help you run that last additional experiment requested...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer Information...

  14. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...this in the future. Here’s a list of blog posts to help you tackle the challenges of DNA repair (with bonus...how a better understanding of Cas9 biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein (RNP)...genome and just stick to RNA? Cas13 may be able to help you do that! How to Design your gRNA for CRISPR ...had great things to say about CRISPOR, a tool to help you design, pick, and close guides for Cas9 and ...

  15. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... While we love academic research, we hope we can help all researchers achieve happiness in their careers...Scientists: Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an...new career path, a career coach might be able to help you. Head Addgenie Joanne Kamens explains what career...engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need...

  16. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...has certainly noticed: This year, the BeHEARD (Helping Empower and Accelerate Research Discoveries) Award...and his colleagues at the Wistar Institute in Philadelphia, PA will put their award to work on a condition...different variants and strategies out there. This will help us acquire a lot of those plasmids so that we can...after periods of high activity. The new work will help them to understand how different mutations lead ...

  17. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...CRISPR, there are two major areas where software can help. The foremost handles the design of sgRNA and represents... Benchling. Utility functions: These functions help speed up the sgRNA design process by removing repetitive...repetitive tasks and/or by providing features to help with the post-design process such as primer and ...applying CRISPR technologies to new organisms and will help you whether you have a full assembly or not. It ...to re-evaluate all tools. Report your findings to help everyone else. CRISPR technology has reached into...

  18. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...these tips and resources collated by Addgenies to help you keep calm and science on. Practice self-care...mental break, while meditating right before bed will help you prepare for sleep. At Addgene, our mindfulness..., you can contribute data to the AAV Data Hub to help the research community. Set expectations for your...overwhelmed right now, but hopefully these tips are helpful to you no matter what you’re trying to accomplish...

  19. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...catalog number for identifying a reagent can be helpful, but it lacks context unless it links to a verified...sequence information, and more. The plasmid page also helps researchers find other publications that have used...Having IDs linked to these metadata and publications help to enable reproducibility of experiments. Additionally...services such as Identifiers.org and N2T.net. To help authors comply with journal policies, Addgene now...

  20. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...bench mentor or anyone else available to directly help you during your visit. Reach out to your bench mentor...comfortable initiating this conversation, your mentor can help get the ball rolling! If you are applying to a structured...Remember that your host is going out of their way to help you with your research and teach you new techniques...questions if you don’t understand something. Offer to help out wherever you can and don’t forget to express...
Showing: 121 - 140 of 753 results