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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 629 results for: EMB

Showing: 121 - 140 of 629 results

  1. Synthetic Biology - Fungal

    Type
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    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  2. Synthetic Biology - Mammalian

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  3. Synthetic Biology - Plant

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  4. Synthetic Biology - Worm

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  5. Synthetic Biology - Yeast

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  6. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...requires deliberate preparation and a willingness to embrace new responsibilities. After my second postdoc,...differencesManaging a team isn’t “one size fits all”. Each team member has unique motivations, strengths, and learning...scientist, I worked with a diverse team where some members thrived on independence, while others required ... stay on track and feel supported. Another team member was highly self-motivated and excelled when given...to the communication style of a new, junior team member who had values, working styles, and skills that...discovery project, I noticed tension between two team members with differing approaches—one detail-focused and...permission to explore, reflect on your priorities, and embrace these transitions as an integral part of your broader...

  7. Synthetic Biology - Strains

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...

  10. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ... Pai, Vaibhav P., et al. "Transmembrane voltage potential controls embryonic eye patterning in Xenopus...slightly slower scale.  All living cells with a membrane possess an electrical state. This state, known...is maintained by ion channels and pumps in the membrane. An emerging interdisciplinary field of biology... depolarized (positive) the cell is, the more embryonic, stem cell-like, or cancerous it is (Levin et ...engineering. Planarian worms cut into three segments “remember” which side should grow a head and a tail, and...features increased brightness and improved plasma membrane specificity. Stable Step Function Opsin - A stabilized...

  11. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post
    ...Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling too ...gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments There...can generate the parts of DNA that you want to assemble together in a combination of different ways according...have a successful and ultimately faster cloning. Remember to quantify the DNA concentration of all the samples...There are multiple ways you can assemble the different parts of a plasmid based on the cloning strategy...

  12. Synthetic Biology - Metabolism

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...

  14. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...permeabilize the cell membrane to allow antibodies to pass through the cell membrane. Formaldehyde fixation...degree of internal complexity such as extensive membranes produces a greater SSC.  Protein expression, measured...targets, measured by fixing and permeabilizing membranes For intracellular protein targets, users must ... dissolving lipids including those of the cell membrane. In some cases, this can mask epitopes such that...propidium iodide, can only enter cells with disrupted membranes as is the case with dead or dying cells. DNA binding...permeabilization as these methods require damaging the cell membrane and will cause all cells to stain positive. If... amine reactive dyes can only pass through the membrane of dead cells. Once in the cells, amine reactive...

  15. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene(s) from Ensembl genome browser: https...https://www.ensembl.org/index.html Design crRNA/ssODN: Using Ensembl Transcript ID(s) as an input query...reagents, we scaled up these tagging experiments by assembling csRNPs in a 96-well plate format and tested parallel...reagents for the knock-in experiment were pre-assembled in a 96-well plate. Glioma stem cells were transfected...be found at Desktop Genetics .For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert...in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal crRNA and tracrRNA using a PCR block...

  16. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ... with SiR-CA (magenta) and outer mitochondrial membrane labeled with SNAP-OMP25 with Atto590-BG (green... 3: Single-molecule tracking of actin filament assembly-regulating proteins in vitro reveals complex events... probes are not very good at crossing the cell membrane. So, you should investigate your options and weigh...self-labeling protein tags are increasingly popular. Embrace the flexibility and switch things up for your next...Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003  Gallagher, S. S., Sable, J. E., ...Biology, 15(2), 128–136. https://doi.org/10.1016/j.chembiol.2008.01.007  Griffin, B. A., Adams, S. R., & ...https://doi.org/10.1038/nbt765  Lavis, L. (2016, September 8). Better Dyeing Through Chemistry & Small Molecule...

  17. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    ...toehold switches for Zika detection We and other members of the lab had been working on advancing the paper-based...Aedes mosquito (Messina et al, 2016). We quickly assembled a team of students, postdocs and professors to...also were careful to set up clear tasks for each member of the team to accomplish so that many elements...Sensor Constructs at Addgene As we report, we have embedded this next generation of toehold switch-based RNA...further develop our rapid prototyping and sensor assembly pipeline for future emerging pathogens or health...synthetic biology, nanomaterials science, and self-assembly for applications in health, energy, and sustainability...Listen to Our Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How...

  18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post
    ...NGS assembles a larger sequence from many smaller sequences, regions with repeats may not assemble correctly...circular assembly. Most of the time, these plasmids are returned as one or two partial assemblies. If we...complete. After the run completes, we begin our assembly process. Again, thanks to our partners at seqWell...from each sample in our pool and individually assembles the reads into a single FASTA sequence that our...that are particularly difficult to sequence and assemble, including GC rich regions. For example, the CAG...

  19. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...and plasma membrane reporters as well as secretory proteins or proteins targeted to membrane sub-domains...cleavage upon co-expression and spontaneous self-assembly. In an effort to obtain even more precise temporal...individual reporter constructs or as the fully assembled pMemoryArray.   Yang et al., Nat Methods 2014... Chem Biol. 2012 Apr 20;19(4):507-17. 10.1016/j.chembiol.2012.02.006. 2 Guntas et al., Proc Natl Acad...arrival in their target compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can...plasmids with MXS-chaining. Analogous to modular assembly methods, such as BioBricks, the MXS-chaining method... organelles, a cell cycle indicator for mouse embryonic stem cells and a series of inducible promoter ...
Showing: 121 - 140 of 629 results