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We narrowed to 1,144 results for: Gene

Showing: 121 - 140 of 1144 results

  1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post
    ...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively...system also enabled them to turn on genes anywhere in the genome for gene control over a 1,000-fold range....control genes, but according to the Broad Institute's Silvana Konermann, the first generation of CRISPR-Cas9...CRISPR-Cas9 plasmids were not designed with gene activation in mind. “We had not managed to create a system...CRISPR-Cas9 activators that work well enough on any gene that they could be used by people with little bioengineering...Konermann said. “If you want to activate all 20,000 genes, it is not possible to test each one and make sure...you are really confident about working for most genes and individual targeting modules. Those two things...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...interested in heftier genes, you can coinfect with multiple viruses to express genes or gene sets over 4.7 kb...capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be 4.7 kb or less to fit in ...expressing multiple small genes, you can design single rAAV vectors with multiple genes packaged in them and...cell-specific gene expression and viral infection. Cre or Flp dependent switches control gene expression ... transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics... AAV is delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used...icosahedral capsid, that can be used to express genes of interest in cell and animal models. AAVs are ...

  3. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...isoform-specific gene expression. Gene isoforms refer to mRNAs generated from the same gene but with different...isoform-specific gene regulation — at least, more straightforward than generating cDNA for each gene isoform individually...first ask yourself whether gene overexpression is necessary (instead of gene knockout or knockdown, for...tools for modulating gene expression in the central nervous system (CNS). To address this technological ...combinations of transcriptional activators to induce gene transcription at genomic sites targeted by specific...libraries. However, using CRISPRa to target individual genes can be relatively laborious because multiple plasmids...packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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    Blog Post
    ...two genes: the replication (rep) gene and the capsid (cap) gene. In wild-type AAV, these two genes are...with BioRender.com   Replication (rep) gene The rep gene encodes four proteins that are required for... is called cross-packaging.  Capsid (cap) gene The cap gene encodes three AAV viral capsid proteins VP1...which contains the AAV structural and packaging genes, the adenoviral helper plasmid which contains the..., freeing up space in the viral genome so that a gene of interest can be packaged into the virus instead...Next let’s take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products...and packaging: Rep78, Rep68, Rep52, and Rep40. Rep gene expression is often driven by the AAV p5 or p19 ...

  5. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...time-consuming, as it only changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells in... to create mice with gene knockouts in their immune systems. However, many genes have different functions...combination with different genes. Having CRISPR systems for assessing genes in defined contexts would ... deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene knockout in immune...Annotation of genes in immune cells typically involves the creation of germline knockout mice, which ...the constitutive, ubiquitous knockout of single genes in immune cells in vivo (LaFleur et al., 2019a and...Mouse Strain C-CHIME Knocks out a pair of genes pXPR_219 Violet-excited GFP (vex) Rosa26-Cas9...

  6. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...both introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site ...first-generation transposase (as determined by transposition assays of antibiotic-resistance genes conducted...). In their Nature Genetics paper, the lab showed that efficiencies of stable gene transfer using SB100x... the rapid generation of stable transgenic cell lines which can robustly express your gene-of-interest... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...transposase enables robust stable gene transfer in vertebrates. Nat Genet. 2009 Jun;41(6):753-61. Epub 2009... et al. A new approach to gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells...

  7. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    Blog Post
    ...planarian genes, we encourage you to deposit them back with Addgene and expand the number of genes that other...expression of specific gene expression. The 49 deposited plasmids enable students to study gene expression and...planarians. For example, the gene Piwi labels neoblast cells and the gene Prohormone convertase 2 (pc2...beta-catenin. Other gene targets do not require amputation to be visible. For example, odf2 gene knockdown results...and functions of key regeneration-related genes in four inexpensive and commercially available planarians...types and for viewing how gene expression changes through time or after genetic manipulation. The technique...Scientists routinely use techniques to alter gene expression or to label specific cells, but there are...

  8. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...antibiotic resistance genes. Genes that are targets of antibiotics are often essential genes which creates a.... ICEBs1 carries the genes needed for conjugation, but can also carry cargo genes, like Mobile-CRISPRi...if that gene is a target of an antibiotic. For example, let’s take a look at the essential gene folA which...targeted essential and non-essential genes with two guides per gene. The library was grown in glucose minimal...essential genes were less represented while bacteria containing guides targeting non-essential genes remained...does not disrupt gene function and is stable and active for greater than 50 generations. Figure ...tools scientists can use to study them. For example, gene knockdown with CRISPR interference (CRISPRi) has...

  9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...replication and viral gene transcription (6). The EBOV genome contains seven viral genes, each flanked by ...sequence (gene start) and a transcription termination/polyadenylation signal sequence (gene end). A minigenome...Figure 1). All viral genes are removed and replaced by a single (non-viral) reporter gene (e.g. firefly luciferase...luciferase or eGFP) flanked by the EBOV-specific gene start and gene end sequences. Thus, the minigenome contains...Cloning the viral genes in this plasmid would be more difficult compared to if each gene were encoded in...transcribe the reporter gene (e.g. firefly luciferase). The polymerase recognizes the gene start signal and ...of the reporter gene. The polymerase will elongate the mRNA until it reaches the gene end signal, where...

  10. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...depending on its orientation to your gene. T7lac High levels of gene expression Promoter from T7 bacteriophage...modulating gene expression through varied inducer concentrations. trp High levels of gene expression...human or mouse) General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive...deacetylation. Ubc General expression mRNA Mammalian promoter from the human ubiquitin C gene Constitutive  ... human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive  Ubiquitous... Requires the presence of Gal4 gene to activate promoter. Ac5 General expression mRNA Strong insect ...Actin 5c gene Constitutive  Commonly used in expression systems for Drosophila. Polyhedrin General expression...

  11. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...the env gene containing the rev response element (RRE), an internal promoter, and the desired gene (transfer...-1 genome containing all viral genes with the exception of the env gene (packaging plasmid), and 3) the...used to express your gene of interest either constitutively or inducibly. If your gene of interest is toxic...expression of your gene. The addition of Dox in the culture will then repress your gene of interest. This... Youichi Suzuki (2011). Gene Regulatable Lentiviral Vector System, Viral Gene Therapy, Dr. Ke Xu (Ed.)...harmful genes into a transfer vector. Lentiviral particles expressing proto-oncogenes or oncogenes for instance...safe use in a clinical setting. In this generation, the HIV Tat gene (previously used to drive expression...

  12. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...70% of human genes have a counterpart in zebrafish. Understanding the function of these genes in fish will...unknown functions for genes, and lays the groundwork for understanding the gene networks that regulate...zebrafish-specific gene expression databases that provide information about when and where genes are active in...investigations. Genetic resources for zebrafish A common approach to unravel the gene networks that drive...model organism is its amenability to genetic manipulation. Tools for gene manipulation together with information...Additional mutagenesis projects have since been initiated and today thousands of mutations in genes affecting...other resources. Targeted gene deactivation tools for zebrafish Random mutagenesis screens provided a major...

  13. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous ... worth codon optimizing a human gene for expression in E. coli. Genes are also optimized for expression...ThermoFischers GeneArt Gene Synthesis NovoPro Labs Codon Optimization Tool (ExoOptimizer) Genewiz Codon Optimization...Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes, so check out our inventory... for a molecular biologist who wants to express genes from one organism in another? Let’s take a look ...correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010...usage frequency in a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created...

  14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...among other things) gene delivery, generating pluripotent stem cells, and developing gene therapies for monogenic...and lentiviral vectors that are used for gene regulation, gene of interest expression, knockout, and screening...Experimental uses include: Cre-dependent gene expression, gene knockout, and selection marker removal. ...Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest into...split-vector systems’ by separating viral genes and transgene expression across several plasmids. The most... host genome and thereby enable long-term stable gene expression. Gamma-retroviral vectors are derived...diseases. Additionally, retroviral vectors can regulate gene expression by delivering shRNA or miRNA. The ever...

  15. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi...each containing multiple gRNAs for each target gene. In a CRISPR screening experiment, target cells ...are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR library may target...target the entire genome or a specific class of genes (subpools). Species - the gRNAs within a given CRISPR...Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman...Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini ...Birsoy 3rd 7–10 2,105 630 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy 3rd...

  16. KLF Research Plasmids

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    Collection
    ...depositing plasmids with Addgene or email deposit@addgene.org to get started. Genes KLF gene pages with information...KLF Plasmids Browse Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...to the establishment of this shared resource on Addgene. If you have created plasmids containing KLF, we...

  17. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI ...PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  18. CRISPR Plasmids - C. elegans

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    Collection
    ...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does...dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  19. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post
    ... on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA editing...Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies, founded on Gray’s work, ...protein 2 (MECP2) gene, Rett Syndrome is a rare, progressive, neurodevelopmental genetic disorder that affects...of Rett Syndrome research, the role of the MECP2 gene, and highlights some of the latest breakthrough ...painstaking work trying to isolate the causative gene for Rett Syndrome, Huda Zoghbi's lab discovered ...the first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We...global transcriptional regulator of thousands of genes. Studies have suggested roles in transcriptional...

  20. Microbiology Resources

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    Collection
    ...page to look for specific genes or applications that are not listed here. Addgene Microbiology Resources ...of Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye...Lab Synthetic Biology BIOFAB plasmid set : E. coli gene expression - Endy Lab CIDAR MoClo Parts Kit : Molecular...Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast ...Resources : Browse plasmids, from networks and gene regulation to sensing and signaling, to perform your synbio...Resources for Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses...
Showing: 121 - 140 of 1144 results