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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 326 results for: INO

Showing: 121 - 140 of 326 results

  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea...formed from a maturation reaction of three specific amino acids at the center of the protein (Ser65, Tyr66...

  2. Promoters

    Type
    Guide
    ...from Lac operon araBad Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible ...adaptor molecules that are responsible for bringing amino acids to the ribosome when proteins are being synthesized...

  3. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections ... Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest ...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016...Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni ...editing than SpCas9 (Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM...

  7. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett...10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  8. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still...expression strain to make sure they are compatible. An arabinose-inducible plasmid will not express in an IPTG ...

  10. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known ... antigen-binding fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding...

  12. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non...fluorescence of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from ...

  13. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...palindromic and repetitive sequences, but if present, a minor extension can usually ensure that the 3’-base(s)... of the most popular approaches for introducing minor modifications to a plasmid template. Many thanks...

  15. mTOR Pathway

    Type
    Collection
    ...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy levels, and growth...PIK3R1 PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and ...

  16. DNA Quantification

    Type
    Protocol
    ...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering Plasmid...

  17. Microbiology Resources

    Type
    Collection
    ...Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. ...assembly of up to three transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway...

  18. Protocol - How to Design Primers

    Type
    Protocol
    ... also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...

  19. TALEN Guide

    Type
    Collection
    ...composed of 33-35 amino acid repeats. These repeats only differ from each other by two amino acids, their ...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...rule of thumb, we avoid target sites that code for amino acids near the N’ terminus of the protein, in order.... Likewise, we avoid target sites that code for amino acids close to the C’ terminus of the protein, to...
Showing: 121 - 140 of 326 results