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We narrowed to 319 results for: INO

Showing: 121 - 140 of 319 results

  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of mulitple ... type or experimental conditions.   Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G ...

  3. Tips for arabidopsis transformation

    Type
    Blog Post
    ...of culture you are using and whether you are inoculating from a frozen stock or a colony from an LB plate...plate. In my hands, a 5 mL starter culture inoculated from a colony on a plate will reach an optimal density...

  5. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...(ADAR). Such a system would convert adenine to inosine, which the translational machinery treats like ... ADAR1 and ADAR2, which deaminate adenosine to inosine in RNA, creating a functional A->G change. They...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    ...Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and ...plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single base pair change...

  7. Plasmids 101: Protein tags

    Type
    Blog Post
    ...affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash for your protein...; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus, promotes...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea...formed from a maturation reaction of three specific amino acids at the center of the protein (Ser65, Tyr66...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni ...editing than SpCas9 (Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016...Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional...

  12. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections ... Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest ...

  13. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett...10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  14. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still...expression strain to make sure they are compatible. An arabinose-inducible plasmid will not express in an IPTG ...

  16. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non...fluorescence of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from ...

  17. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known ... antigen-binding fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding...

  19. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...palindromic and repetitive sequences, but if present, a minor extension can usually ensure that the 3’-base(s)... of the most popular approaches for introducing minor modifications to a plasmid template. Many thanks...
Showing: 121 - 140 of 319 results