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We narrowed to 323 results for: INO

Showing: 121 - 140 of 323 results
  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...” column. Relevant Mutations Indicate the amino acid change as well as any functional consequences...hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of...
  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems are ideal for... to assemble proteins with labelled or modified amino acids that are useful in different downstream applications...
  3. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...often doubles the volume of culture used for inoculation if we get inadequate plasmid DNA yields. Why ...growth conditions, and the material used for inoculation. To further complicate matters, plasmid copy ...
  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of mulitple ... type or experimental conditions.   Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G ...
  5. DNA Quantification

    Type
    Protocol
    ...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering Plasmid...
  6. Protocol - How to Design Primers

    Type
    Protocol
    ... also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...
  7. Tips for arabidopsis transformation

    Type
    Blog Post
    ...of culture you are using and whether you are inoculating from a frozen stock or a colony from an LB plate...plate. In my hands, a 5 mL starter culture inoculated from a colony on a plate will reach an optimal density...
  8. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...(ADAR). Such a system would convert adenine to inosine, which the translational machinery treats like ... ADAR1 and ADAR2, which deaminate adenosine to inosine in RNA, creating a functional A->G change. They...
  9. CRISPR Antimicrobials

    Type
    Blog Post
    ...Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and ...plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single base pair change...
  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ...affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash for your protein...; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus, promotes...
  11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea...formed from a maturation reaction of three specific amino acids at the center of the protein (Ser65, Tyr66...
  12. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections ... Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest ...
  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni ...editing than SpCas9 (Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM...
  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016...Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional...
  15. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett...10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...
Showing: 121 - 140 of 323 results