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We narrowed to 492 results for: LARGE;

Showing: 121 - 140 of 492 results

  1. TALEN Guide

    Type
    Collection
    ...sequence ( Figure 1A ). Scientists have identified a large number of zinc fingers that recognize various nucleotide...

  3. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...the advent of CRISPR/Cas9 technology, inserting large tags into a precise location in the genome is still...fact. Later, when we transfect the cells, these large regions of homology enable us to effectively utilize...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...genome edits, but it is effective at wiping out large regions of DNA and has anti-viral application potential...facts – PAM requirement: AAG or TTC  Best for: very large deletions.   Cas12a – The staggered cutter  Originally...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...affinity reagents is their size. Antibodies are large enough that their bulk can be a barrier at times...difficult to source, may not be readily available for a large number of targets, and can require specific conjugates...

  6. To Each HIS Own

    Type
    Blog Post
    ...Moks 1987). These tags, however, tended to be very large and often required the incorporation of a cleavage...M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like...

  7. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...strategy designed by synthetic biologists to perform large scale, one-tube cloning reactions. In this blog,...this sounds really neat — but when is this type of large-scale assembly useful, you may ask? You might be...put, it assembles individual DNA components into larger units or circuits with functional relevance following...MoClo enables individual parts to be assembled into larger functional units which are transcribed together...translational output, etc., which can support a larger cellular engineering goal, several of which are...

  8. CRISPR 101 eBook...en español

    Type
    Blog Post
    ... 101: Una herramienta de edición  Addgene has a large international audience, including working with distributors...

  9. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... some of the limitations of viruses. TEs have a large payload, up to 100 kB with Sleeping Beauty and piggyBac...payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, ...transposons to allow for the targeted delivery of large DNA cargos (>100 kB) to specific locations in the...

  11. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...heavy machinery. But not all engineering is at a large scale — some is done on much smaller equipment on...generation. To evaluate improved properties across large mutant libraries, high-throughput screening techniques... Innovation (IPI) provides information on their large-scale recombinant antibody production platform. ...depth is antibody directed evolution. This is a larger process of gradually changing and selecting the...

  12. Lab Automation at Addgene

    Type
    Blog Post
    ...the Biomek FxP is it’s customizability. It has a large deck that can house many different types of devices... DNA purification We're developing ways to prep large quantities of DNA because some countries just can...be used for a mini prep, except it does it on a larger scale. Using the BIOROBOT Universal Platform is...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    ...the target gene’s native locus, but introducing large genetic changes in C. elegans with CRISPR can be...(ssODN). So how can you use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach...

  14. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... immunoglobin component that can be produced in large “copies.” This homogeneity circumvents the batch-to-batch...reproducibility and cost-effectiveness for broad and large quantity uses. We therefore use both rAb and mAB...as non-human primates and humans. Since we need larger-volume antibodies for thicker tissues and whole...

  15. p53 Pathway

    Type
    Collection
    ...only one functional copy of the p53 gene causes a large reduction in tumor suppression activity, leading...

  17. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...In complex metazoans, rapid cell division and large scale cell mobility are essential processes during...body. In contrast, these dynamic processes are largely absent in adult organisms, where tissues structures...adapted to studying other systems in which there is large scale cellular movement throughout an organism. ...

  18. Common Injection Routes in Mice

    Type
    Blog Post
    ...injection site is straightforward, as it targets large muscle groups (usually the thigh), though it is ...theoretically be performed anywhere on the body, somewhere large, flat, and easily accessible (like the back) is ...

  19. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...population does not move even after applying very large compensation values (100+), consider what two markers...diagonal line in the top right corner of the plot and large compensation values will not move it (Figure 3)....
Showing: 121 - 140 of 492 results