We narrowed to 183 results for: Lta

1. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ...Any IVA primers can be used in combination to simultaneously perform modifications, simply by adding all...

2. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ... shrink the construct. They identified ADAR2DD(delta984-1090), which decreases the REPAIRv1 construct ...

3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   Blog Post

   ...mentioned above, organic solvents can be used to simultaneously fix and permeabilize a sample. But you can ...

4. Droplet Digital PCR for AAV Quantitation

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   Blog Post

   ...measure the concentration of multiple targets simultaneously. For AAV titration, you should only need to...

5. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

   ...Kamuzinzi, R., Ledent, V., Blaudez, D., Sanders, D., Revuelta, J.L., Boles, E., André, D., and Frommer, W.B....

6. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ...PMCID: PMC6412672. Zhou, Haibo, et al. "In vivo simultaneous transcriptional activation of multiple genes...

7. Analyzing CRISPR Editing Results with ICE from Synthego

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   Blog Post

   ...analysis, which compares hundreds of samples simultaneously.  The current version of ICE software can analyze...

8. Overcoming the AAV Size Limitation for CRISPR Delivery

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   Blog Post

   ...Constructs containing the ADAR2 truncation ADAR2DD(delta984-1090) are approximately 4.1 kb in length, allowing...

9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...

10. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    Blog Post

    ...Additionally, we showed that TnpB can be used to simultaneously target multiple genes, enabling efficient multiplexed...

11. Hot Plasmids: Fall 2024

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    Blog Post

    ...target multiple genes in M. abscessus either simultaneously or stepwise, achieving mutation efficiencies...

12. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector...

13. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

    ...from the cell. Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates...

14. Genomic Deletions in Mammalian Cell Lines

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    Collection

    ...plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not...complement oligo ( e.g., sgRNA-A in Table 4 ). The resultant oligos would be 25-mer oligos. However, if the...the reverse complement oligo. In this case, the resultant oligos would be 24-mer oligos ( Table 4 ). Design...

15. Five Popular Model Organisms

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    Blog Post

    ...often as every three weeks so scientists can simultaneously observe several generations of mice at a time...

16. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post

    ...of artificial guide RNAs that can be used to simultaneously target cellular RNA to specific intracellular...

17. Five Popular Model Organisms, Part 2

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    Blog Post

    ...and easily obtainable growing conditions. As a facultative aerobe, it can thrive with or without oxygen....

18. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

    ...pathway. Accomplishing this usually involves simultaneously introducing a homologous DNA repair template...

19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect...

20. CRISPR 101: Validating Your Genome Edit

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    Blog Post

    ...main advantage of NGS is that it allows you to simultaneously validate edits and look for off-target changes...