We narrowed to 140 results for: NUA
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TypeBlog Post... I needed to be close on a subject, or I could manually control the camera by hand and zoom from a distance...
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CRISPR Library Amplification
TypeProtocol...follow all specifications described in the equipment manual. Immediately add 1 mL SOC to cuvette. Remove all... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog Post...Tsien R. Y. (1998). The green fluorescent protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...expertise! This post was originally published in January 2025 and updated in August 2025. References and... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...published by Simpson, Sacher, and Szymanski in January 2016 in Viruses (5). Please refer to the paper ... -
Transferable Skills Guide: Teamwork
TypeBlog Post...No single person could possibly understand every nuance of how kits work at Addgene, but with the help ... -
9 tips for a successful postdoctoral experience
TypeBlog Post...lasts five years. Most universities and institutes annually advertise for Group Leader positions starting ... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...undergoing RNAi through the fluorescent reporter and manually extracted. This process is tedious and time consuming... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...acceptors in their product descriptions, or technical manuals. Specific applications and matching dye/ligand... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...excluding out of focus light. However, they also continually dose the sample from top to bottom with excitation... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...The development and application of optogenetics. Annual review of neuroscience. 2011;34:389-412. doi: 10.1146... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...with a purification, reading the full instruction manual will help explain the rationale behind each step... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post... (2017). CRISPR-Cas9 Structures and Mechanisms. Annual Review of Biophysics, 46, 505–529. https://doi.... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...article was originally written by Tyler Ford in January 2016. It was updated by Angela Holmes and Christina... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post... join professional organizations and attend the annual conferences – i.e. Society for Neuroscience for... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...quite clear that others thought the same. Since January 2013 there have been 33 CRISPR software tools published... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol.... RNA . 2003. 9(4):493-501. PubMed . Multiply attenuated lentiviral vector achieves efficient gene delivery... -
Plan Your Experiment
TypeGuide...While there are some cases where it makes sense to manually design a gRNA, in most cases gRNA design software... -
Lentiviral Vector Guide
TypeGuide...J., Naldini, L., & Trono, D. (1997). Multiply attenuated lentiviral vector achieves efficient gene delivery... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...At Addgene we're continually impressed with the amazing plasmid technologies developed by our community...