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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 365 results for: Pol;

Showing: 121 - 140 of 365 results

  1. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of...topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated insert. When mixed, topoisomerase I... could also be a great choice, because the T4 polymerase needed for this method is relatively inexpensive...

  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... can then be translated at the ribosome into polypeptide chains that make up the primary structure of ...purified for use in molecular biology research (eg. polymerases and other enzymes might be purified and used ...translation machinery. These include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free...

  3. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Addgene blog   Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview and Applications...Applications Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer...SYBR Green I and TaqMan quantitative real-time polymerase chain reaction methods for the determination ...

  4. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...versus the response and use that information to extrapolate the concentration of an unknown sample. For example...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample based...sample is determined by using the trendline to extrapolate its x value, 0.963 mg/mL, from its y value, 0.651...

  5. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...set up a standard PCR reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template...standard PCR reaction: combine template, primers, polymerase, and dNTPs and then incubate with a standard ...heating step of the PCR reaction. A standard Taq polymerase is sufficient. Controls: Controls can make ...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...centrifugation and then by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then ...lentiviral preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated...

  7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses... inactivated by the complement system in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping...–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL...

  10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...with a “sloppy” version of PCR that results in a polymerase error rate of up to 2% per nucleotide position...modifications: 1) increased concentration of Taq polymerase, 2) increased PCR extension time, 3) increased...degenerate primers and the high-fidelity Phusion polymerase are used to introduce point mutations into the...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...of useful, entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking...almost anyone with enough practice. I always take a poll of scientists in training to see how many in my ...other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular question...

  13. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...high-fidelity polymerase to ensure accuracy. In this case, you would want a low-fidelity polymerase, like Taq...forcing sequence diversity using error-prone polymerases, you force sequence diversity using a pool of...

  15. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...cation channel, activation of which results in depolarization and subsequent firing of an action potential...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through hydrogen...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...proteins whose fusion improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding ...rXRCC1, or the DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion...

  18. Caltech Systemic Capsids

    Type
    Collection
    ...Dimidschstein 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Control Feng 163505 CN1390-rAAV-DLX2.0...

  19. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Bradley 3rd ~9 pairs/exon 9,508 Mouse CRISPR Proximal Poly(A) Site Knockout Library 190744 Knockout Mouse Bradley...

  20. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...with BioRender.com. Top7 gets its name from “topology of protein” diagrams, which show the interactions...seen so far!). They started by designing a novel topology, then iteratively generated 3D models and amino...
Showing: 121 - 140 of 365 results