...with a “sloppy” version of PCR that results in a polymerase error rate of up to 2% per nucleotide position...modifications: 1) increased concentration of Taq polymerase, 2) increased PCR extension time, 3) increased...degenerate primers and the high-fidelity Phusion polymerase are used to introduce point mutations into the...

...Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio...Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large...

...of useful, entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking...almost anyone with enough practice. I always take a poll of scientists in training to see how many in my ...other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular question...

...from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in... in Biology and Biotechnology from Worcester Polytechnic Institute. What is RNAi? The loss-of-function...

...cation channel, activation of which results in depolarization and subsequent firing of an action potential...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through hydrogen...

...proteins whose fusion improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding ...rXRCC1, or the DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion...

...controlled by the regulatory sequences of an RNA polymerase III transcribed U6 snRNA. In both vectors, the...

... a gene, it blocks the progression of the RNA polymerase, thus strongly decreasing the expression level...

...enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting...

...Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids...

...be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is available ...

... OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc apoeb (apolipoprotein Eb) OZ529 and OZ530 OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg...

...with BioRender.com. Top7 gets its name from “topology of protein” diagrams, which show the interactions...seen so far!). They started by designing a novel topology, then iteratively generated 3D models and amino...

...walking tour of Buddy the Elf’s journey from the North Pole to New York City. Check out their video below. ...interpretation of Buddy the Elf's journey from the North Pole to New York. #ELF #DeckTheLab #ProudPIhttps://t....

...interfering with transcriptional elongation, RNA polymerase binding, or transcription factor binding. The...

...biorender.com.    Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein...

...amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...message delivered by many of the speakers.  As Dr. Polka said as she started things off, “We are all capable...

...Krzysztof Chylinski (who coincidentally speak the same Polish sub-dialect making their Skype communications extra...evolution A Powerful New Way to Edit DNA (by Andrew Pollack of New York Times)   ...

...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

...Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide Alters outer membrane permeability Bactericidal...