We narrowed to 225 results for: SIR

... another way, only cells or organisms with the desired phenotype will survive and be assayed at the end...

...before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some of these synthetic...

...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....

...both tissue or cell type, amount of protein, and desired fragment length for your output assay. A longer...

...different Cas enzymes to make sure you are getting the desired features and cutting (note: spCas9 isn’t always...

...delivery of the actual virus to which immunity is desired. Instead, components of the virus are now delivered...

...mutagenesis. During this process, incorporation of a desired mutation into your plasmid of interest by PCR generates...

...ideally be optimized to promote generation of the desired product while avoiding the accumulation of toxic...

...Their His tags allow for further purification, if desired, but it's not required for their use. Each RANbody...

...transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both...

...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...

.... References 1. Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott, M., & Devenish, R. J. (2008)....

...them construct “designer” channelrhodopsins with desired characteristics. The model was trained to predict...

...expression. This system could be used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown...

...guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion ...

...essential proteins (when knockouts aren’t viable). siRNA or RNAi are small, double stranded RNA molecules...

...using a resistance marker; however, it is often desirable to remove the marker after the initial selection...

...literature and offer educated insight into what the desirable features of future technologies should offer in...

...choice for FRET assays where a dark acceptor is desired due to its very low quantum efficiency. ShadowY...

... that links readers to more information if they desire it. Shapes. In this type of poster, written and...