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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19 results for: SIR

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Showing: 1 - 19 of 19 results

  1. Mammalian RNAi Tools

    Type
    Collection
    ... binds the shRNA and cleaves it to produce siRNA. The siRNA then binds the RNA-induced silencing complex...double-stranded siRNA and degrades one of the strands. The remaining single-stranded siRNA then binds the...can be delivered as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur via ... facilitates cleavage of the target. RNAi using siRNA follows a similar mechanism. Since it is pre-processed...J. (2009). Origins and mechanisms of miRNAs and siRNAs . Cell, 136 (4), 642–655. PubMed (Link opens in...Molecular mechanisms and biological functions of siRNA . International Journal of Biomedical Science, 13...Vorhies, J. S., Senzer, N., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced...

  2. Plan Your Experiment

    Type
    Collection
    ...experiment. Created with BioRender.com. Select Your Desired Genetic Manipulation Different genetic manipulations...Activate Design and Clone Your gRNA Achieving your desired edit comes down to location, location, location...site as close as possible to the location of the desired edit, ideally less than 10 bp away. For more information... cases, there may be no PAM site close to your desired edit site. Consider alternative Cas9 enzymes with...sites . Base Editing : Target the site of your desired edit. Many base editors exert their functions within...consider factors that maximize cleavage of the desired target sequence, or on-target activity. Two gRNA...how to design your gRNA . Synthesize and Clone Desired gRNAs Once your target sequences are selected, ...

  3. CRISPR Guide

    Type
    Collection
    ... the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit...) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired edit is incorporated...cell containing the CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing...may not be positioned correctly to target your desired genes for modification. Engineered SpCas9 enzymes...donor template . The donor template contains the desired sequence and is delivered to the cell along with...(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional DNA matching the sequence...will improve the accuracy and efficiency of the desired edit. For example, you should alter the PAM site...

  4. CRISPR References and Information

    Type
    Collection
    ...vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF, 108...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Voeltz 226567 siRNA-resistant SigmaR1-mCherry SIGMAR1 mCherry CMV ALS Gia Voeltz 226569 siRNA-resistant pLV-SigmaR1...226571 siRNA-resistant SigmaR1-mNeonGreen F191A SIGMAR1 mNeonGreen CMV ALS Gia Voeltz 226572 siRNA-resistant...226573 siRNA-resistant SigmaR1-mNeonGreen D188A SIGMAR1 mNeonGreen CMV ALS Gia Voeltz 226574 siRNA-resistant...ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human...ss-SigmaR1DTM-mNeonGreen SIGMAR1 mNeonGreen CMV ALS Gia Voeltz 226576 siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A SIGMAR1... SIGMAR1 mNeonGreen CMV ALS Gia Voeltz 226577 siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A...V190A) SIGMAR1 mNeonGreen CMV ALS Gia Voeltz 226578 siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A ...

  6. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...also encodes a primer binding site (PBS) and the desired edits on an RT template. During prime editing, ...3’ flap binds to the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription...

  10. TALEN Engineering

    Type
    Collection
    ... engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation...

  11. Viral Vector Guides and Plasmids

    Type
    Collection
    ...the production of viral vectors that contain the desired genetic cargo. In turn, these viral vectors are...

  12. mTOR Pathway

    Type
    Collection
    ...Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov. 2015 Apr;14(4):261-78...

  13. Worm Expression Resources

    Type
    Collection
    ...characterized which allow researchers to express desired constructs in precise cell types and/or developmental...

  14. Plant Plasmids and Resources

    Type
    Collection
    ...of microRNAs (miRNAs) or small interfering RNAs (siRNAs) to achieve gene silencing in plants. It also includes...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...transduction (transient or stable) may depend on the desired sensitivity, level of induction, or amount of background...

  18. Brain Initiative Collection

    Type
    Collection
    ...brain cells and circuits during behavior. Long desired by researchers, the revolutionary dynamic picture...
Showing: 1 - 19 of 19 results