We narrowed to 424 results for: SUA
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TypeCollection...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...
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Cre-Lox and Other Site-Specific Recombinases
TypeCollection...protein into your expression vector allows you to visualize which cells have undergone recombination. Reporter... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post...that many people honor. Talking with strangers is usually challenging but does get easier with practice. ... by meeting with people working in diverse and unusual fields. My favorite final trick is to end each ... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs in vitro, you can send us... have data for are: cut, activate, interfere, visualize, nick, purify, tag, scaffold, CRISPR-display, ... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog Post... the paper. Delivery of AAV into the brain can usually be achieved by a directed brain injection, but ...strategies can also be used for retrograde tracing: visualizing neurons that received information from a region... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...difficult to study without biosensors to directly visualize their activity. In 2019, the first generation ...developed, named GRABNE, which helped researchers visualize norepinephrine (Feng et al., 2019). But this first... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...labeling; multicolor labeling would enable the visualization of multiple loci. Such a system could then be...major applications to cancer, for example, the visualization of chromosomal translocations or chromosomal... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...to apply for a position. A job description is usually a wish list and the hiring manager knows that no... know the exact person monitoring submissions (usually a Human Resources representative). Pro tip: if ... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...imaging with fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail... lux. arg1 and lux reporters both allow for visualization of the bacteria, but lux imaging shows only ... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post... file into the desktop version of Snapgene to visualize the sequence and look for features. The first ...extensive database. Snapgene also provides easy visualization of open reading frames, which is often our first... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...techniques and cool plasmids. Creating graphics were usually the last thing I thought about when writing posts...file size with a Biorender watermark, which is usually ok for informal settings like lab meeting presentations... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post... called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers (collectively known...optimal Rluc8-Gα construct for two of the 16 non-visual G protein transducers. To use the TRUPATH system... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...labeling fewer cells in a population, it’s easier to visualize individual/non-overlapping cells. While transgenic...Drosophila, the SPARC systems can be used both for visualization and for optogenetics. The Clandinin lab used... -
Build Your CRISPR Vocabulary
TypeBlog Post...use an RNA guide to target other nucleic acids—usually DNA—to cut, nick, or bind. DNA CRISPR array: The...previously encountered foreign nucleic acids. The unusual sequence of these arrays, with alternating spacers... -
Year of the Camelid: Antibody Style
TypeBlog Post...genetically fused to fluorescent proteins and used to visualize target antigens in living cells or tissues. sdAbs...the process started with immunizing a camelid (usually llamas or alpacas). But because sdAbs are so amenable... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...PMID 29379139. The “Riboglow” platform for visualizing RNA production and localization Article contributed...Riboglow,” a set of tools that can be used to visualize RNA localization in mammalian cells. These tools... -
Viral Vectors 101: Transductions
TypeBlog Post...delivery to be viral-mediated. Transformations are usually bacterial in nature. All three of these methods...packaging plasmids into a viral production cell line, usually HEK-293 cells. When all of these viral plasmids... -
Antibodies 101: Epitope Availability
TypeBlog Post...working with antibodies.) Conformation changes usually fall into one of two categories: ones induced by...or fold in certain conditions? It can help to visualize an abstract protein during your sample prep process... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...seminar speakers. My eye was caught by a couple of unusual job openings, although they seemed too far afield... a look at our news editors. A fondness for visualization of science? Look at our graphics and design ... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...protein, such as GFP, which allows you to visualize the cellular localization of your protein...