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  3. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  4. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    ...their BioRxiv post, the authors generated and described several improved genetically encoded fluorescent...Takayuki Iwaki, Kimiko Hara, and Kazuo Umemura have described a simple method which alleviates the inclusion...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...Cas13a as a diagnostic tool, as discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   ... the collateral RNA cleavage response is well described in bacterial cells, LwaCas13a does not display...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...positive control, the internal control vector, was described earlier. Once you are sure your conditions are...come from multiple, independent sources. We'll describe these a bit more below, but the papers cited in...

  12. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...addition of IPTG. T7 RNAP is then available to transcribe the gene of interest from a T7 promoter on the...don't see protein overexpression? The strains described above should generate sufficient expression levels...

  13. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, PAM requirements, experimental...

  14. TALEN Engineering

    Type
    Collection
    ...for FLASH assembly. Links to additional pages describing these reagents are provided below: Joung Lab ...

  16. Mammalian RNAi Tools

    Type
    Collection
    ...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme...

  17. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  18. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...systems by LR clonase reaction. These plasmids are described in: Multiplexed activation of endogenous genes...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ..., and ultraviolet light. In this post, we will describe the mechanism of homology directed repair, one...(Quadros et al., 2017). Easi-CRISPR in vitro transcribes RNA encoding your repair template, then uses ...
Showing: 121 - 140 of 382 results