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We narrowed to 616 results for: TIL

Showing: 121 - 140 of 616 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...be visualized by running your PCR on a gel. It’s still paramount that you sequence clones to verify that...homozygous clones, however, sequence confirmation is still needed to verify the correct sequence was inserted...it requires a little extra wet lab work, this is still a great option, especially if the options outlined...data analysis. If NGS is not an option, you can still detect off-target effects with Sanger sequencing...

  2. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...the office had partially reopened. But there were still many restrictions in place, most importantly, masks...would allow me to set up a camera in a room, but still have control over focus, zoom, stop/start record... to mount a battery to it if necessary. I could still control the camera from a distance if I needed to...in-lab filming and remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...repair or add in disease-causing mutations, but, until recently it hadn’t been used to treat disease postnatally...the amount of research conducted on DMD, there is still no good treatment. The dystrophin gene is very large...approach. Since shorter forms of dystrophin can still be functional, exon skipping is a good option for...CRISPR therapy closer to the clinic, much work is still needed. First, CRISPR delivery must be optimized...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, piggyBac™ has ... TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely seamless...piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor plasmid carrying...researcher may subsequently kill off any cells that still contain the donor plasmid after excision by PBx,...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...specific agonists for orphan GPCRs demonstrating the utility of this platform for drug discovery. Researchers...CCP); the latter of which greatly enhances the utility of this collection as it can be used to easily ... allows for easy assembly of DNA fragments by utilizing regions of sequence homology (usually 30-40bp ... are available at Addgene. Since GMAP assembly utilizes 5 different overlap sites, this technique also... repeats into a single-tube, 3 day process by utilizing pre-assembled trimers to reduce the number of ...appealing tool for studying essential proteins. Until recently, it was challenging to apply the AID-tagging...

  6. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...molecules are then activated in the same manner, until enough molecules have been analyzed to allow for...conventional FPs for super-resolution imaging, they still do not emit the large number of photons achievable... the scientific community, and new variants are still being engineered. By combining PA-FPS with superresolution...Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... or topic of interest. Despite its power and versatility, the technique is remarkably straightforward ...dependent on the promoter of the cre recombinase while still taking advantage of the robust expression via the...best to spend the time practicing your injections until they are accurate and reproducible. I also highly...likely be less than that of viral expression but may still be sufficient for successful stimulation. Testing...

  8. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...obvious from our most requested plasmid, SpCas9 is still going strong as the basis for many genome editing... to understand how to use it. TLDR: This is a versatile mammalian expression vector that you can use to...transfection by western blot. Overall, this is a versatile mammalian expression vector that you can use to...

  9. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...expression, though it is possible for the plasmid to still express without the G. Because the G is only one...the efficiency will be lower, although this might still be okay if you are introducing a selection marker...if/when you use HercII or Kapa Hifi the reaction still does NOT work, you can try these new primers: EMX1...Mukherjee, K., & Carroll, D. (2013). Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases...

  10. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...to keep your deposit on hold from public access until the article is published. This policy gives us time... fastest way for us to release your plasmids is still for you to notify us as soon as your article is ...a project isn’t working out as planned, but you still hope somebody will find the plasmids useful, we’...

  11. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...is a great option because it provides a great versatility on how to use your plasmid in every type of experiment...

  12. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...some of these common pitfalls of management and still not get the most from your team. It is pretty clear...personal growth do not exist or if they feel underutilized in their present positions By removing the...will address more specific tactics managers can utilize to be in touch with the needs of the team and to...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies...that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper...Chatterjee A, Sun SB, Furman JL, Xiao H, Schultz PG. A versatile platform for single- and multiple-unnatural amino...

  14. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...tips for scientists working at home, and you will still find this useful. Now that we’ve been doing this...you are more desperate or needy so you can’t wait until you imminently need a job. Start now. Informational...edit everything you’ve tried to draft, but you’ll still be practicing and getting familiar with the process...

  15. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...episome seemed appropriate, especially since the Fertility, or F-factor discovered by Ester Lederberg in ...chromosome in some cases. This terminology held until the 1960s when scientists began to study other extrachromosomal...Although discovered in the early 1950s, it took until the 1970s for plasmids to gain prominence in the...

  16. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...accordingly until we had a model that was viable.  This is not to say that we aren’t still pitching for...Although many emails resulted in no response, I was still able to get through to a number of experts, including...

  17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex ...off switch The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter... By using an orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding...

  18. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...neuroengineering, and some of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering ...focus on the next round. Addgene: What tools are still missing from the optogenetics toolbox? Boyden: That's...question. We've made a lot of progress. I would say we still would like to find ways to make these tools more...

  19. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...learning about biology in high school, it wasn’t until I started working in my first molecular biology ... like taking an engine out of the car – you can still use the engine as a power source, but now you don...pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production proceeds. ...

  20. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...played enormous roles in enabling researchers to utilize and develop these tools by making their own fluorescent...loops on either side of an enzyme’s active site and still block enzyme function upon exposure to ultraviolet...Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety of research applications...
Showing: 121 - 140 of 616 results