...repair or add in disease-causing mutations, but, until recently it hadn’t been used to treat disease postnatally...the amount of research conducted on DMD, there is still no good treatment. The dystrophin gene is very large...approach. Since shorter forms of dystrophin can still be functional, exon skipping is a good option for...CRISPR therapy closer to the clinic, much work is still needed. First, CRISPR delivery must be optimized...

...mutations, we still weren’t entirely happy with our editing outcomes and in many cases still didn’t get ...University in NYC. The CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms... be screening a total of a few hundred clones - Still better than 10.000, right? (Kwart, Paquet, et al...

... too many moving parts in the whole process to distill a one-size-fits-all recipe for success. What I ...the Dean, and even some people in your field that still need to understand your specific questions and experiments...directly state your official aims, but I suggest distilling them into simple goals/outcomes. This isn't the...show interest in other faculty members’ research. Still a Grad Student? Get Tips on Getting the Most out...

...be visualized by running your PCR on a gel. It’s still paramount that you sequence clones to verify that...homozygous clones, however, sequence confirmation is still needed to verify the correct sequence was inserted...it requires a little extra wet lab work, this is still a great option, especially if the options outlined...data analysis. If NGS is not an option, you can still detect off-target effects with Sanger sequencing...

...used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, piggyBac™ has ... TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely seamless...piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor plasmid carrying...researcher may subsequently kill off any cells that still contain the donor plasmid after excision by PBx,...

...specific agonists for orphan GPCRs demonstrating the utility of this platform for drug discovery. Researchers...CCP); the latter of which greatly enhances the utility of this collection as it can be used to easily ... allows for easy assembly of DNA fragments by utilizing regions of sequence homology (usually 30-40bp ... are available at Addgene. Since GMAP assembly utilizes 5 different overlap sites, this technique also... repeats into a single-tube, 3 day process by utilizing pre-assembled trimers to reduce the number of ...appealing tool for studying essential proteins. Until recently, it was challenging to apply the AID-tagging...

...molecules are then activated in the same manner, until enough molecules have been analyzed to allow for...conventional FPs for super-resolution imaging, they still do not emit the large number of photons achievable... the scientific community, and new variants are still being engineered. By combining PA-FPS with superresolution...Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy...

... or topic of interest. Despite its power and versatility, the technique is remarkably straightforward ...dependent on the promoter of the cre recombinase while still taking advantage of the robust expression via the...best to spend the time practicing your injections until they are accurate and reproducible. I also highly...likely be less than that of viral expression but may still be sufficient for successful stimulation. Testing...

...obvious from our most requested plasmid, SpCas9 is still going strong as the basis for many genome editing... to understand how to use it. TLDR: This is a versatile mammalian expression vector that you can use to...transfection by western blot. Overall, this is a versatile mammalian expression vector that you can use to...

...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...is a great option because it provides a great versatility on how to use your plasmid in every type of experiment...

...some of these common pitfalls of management and still not get the most from your team. It is pretty clear...personal growth do not exist or if they feel underutilized in their present positions By removing the...will address more specific tactics managers can utilize to be in touch with the needs of the team and to...

... replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies...that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper...Chatterjee A, Sun SB, Furman JL, Xiao H, Schultz PG. A versatile platform for single- and multiple-unnatural amino...

...tips for scientists working at home, and you will still find this useful. Now that we’ve been doing this...you are more desperate or needy so you can’t wait until you imminently need a job. Start now. Informational...edit everything you’ve tried to draft, but you’ll still be practicing and getting familiar with the process...

...episome seemed appropriate, especially since the Fertility, or F-factor discovered by Ester Lederberg in ...chromosome in some cases. This terminology held until the 1960s when scientists began to study other extrachromosomal...Although discovered in the early 1950s, it took until the 1970s for plasmids to gain prominence in the...

...accordingly until we had a model that was viable.  This is not to say that we aren’t still pitching for...Although many emails resulted in no response, I was still able to get through to a number of experts, including...

...engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex ...off switch The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter... By using an orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding...

...neuroengineering, and some of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering ...focus on the next round. Addgene: What tools are still missing from the optogenetics toolbox? Boyden: That's...question. We've made a lot of progress. I would say we still would like to find ways to make these tools more...

... alcohol or acetone drop by drop onto the smear until the alcohol runs clear and immediately rinse. Flood...for 30 - 60 seconds. Rinse the slide in tap water until the runoff is clear, blot gently to dry, and then...by the interaction of CV+ and I- in the thick multilayered membrane of the gram positive bacteria. In a...

...limitation. The procedure will also need to be perfected until the efficiency of repair approaches 100 percent.... fundamental questions about CRISPR/Cas biology still left to answer as well, she says, and those details...increasing effectiveness of CRISPR/Cas9, scientists are still looking for ways to diminish the possibility of ...

...played enormous roles in enabling researchers to utilize and develop these tools by making their own fluorescent...loops on either side of an enzyme’s active site and still block enzyme function upon exposure to ultraviolet...Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety of research applications...