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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 398 results for: aga

Showing: 121 - 140 of 398 results

  2. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...each one requires different elements for vector propagation. This post, along with a future companion post...yeast plasmid) allows for 50+ copies to stably propagate per cell. The copy number of these vectors can...

  3. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...achieve my purpose? Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's quickly... freezes and then crashes. You’re muted on Zoom again.  These things don’t just happen to you or me (though...

  4. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...for every assay. Antibodies that are generated against a linear antigen tend to work very well for applications...compare the staining patterns of multiple antibodies against the same target; tagged protein strategies that...

  7. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...PhDs Later this week, postdocs and students will again gather in Boston - this time with the aim of finding...University, has recently published an article arguing against reducing the number of graduate students accepted...

  9. 6 Tips for Grant Writing

    Type
    Blog Post
    ...yourself a bit of a break before you read over it again with fresh eyes and correct it (and, believe me,...second) you won’t succeed, but you must try, try again. Now, this does not mean that you should apply for...

  10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...landmark study showed significant clinical efficacy against a form of blindness known as Leber’s congenital...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to...

  11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...microcontroller and a motor controller and made it spin again!  We’ve also learned to be resourceful and to repurpose...Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and the reagents are too ...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...detect mEPSPs, it also enabled imaging of their propagation along dendrites for the first time (Figure 3)...Ashley Waldron We recently added two antibodies against important targets for neurodegeneration research...

  14. Of Myc and Men

    Type
    Blog Post
    ...over the years, several alternative antibodies against the Myc tag have been generated in an attempt to...al.’s results mean the end of 9E10’s time in the saga of the Myc tag? Hardly! 9E10’s accessibility and...

  15. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...negative selection marker HSV-tk is used to select against random recombinants.   Design your targeting...recombination, the negative selection marker selects against improper, random recombination into a different...

  17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...Mobile-CRISPRi stably integrates into the genome and is propagated during normal DNA replication and cell division...recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients with an integrated...

  18. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...affected by the plasmid size, nature of the insert, propagation strain, growth conditions, and the material used...Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and using ...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... used for recombinant antibody production is Komagataella phaffii (a.k.a  Pichia pastoris) because it ... density of the antibody bands can be measured against the protein standard and the concentration may ...
Showing: 121 - 140 of 398 results