We narrowed to 398 results for: aga

...His work focuses on the enzyme hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase ...mouse cortex. Image from He Tian. Congrats again to all the recipients! ...

...PhDs Later this week, postdocs and students will again gather in Boston - this time with the aim of finding...University, has recently published an article arguing against reducing the number of graduate students accepted...

...landmark study showed significant clinical efficacy against a form of blindness known as Leber’s congenital...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to...

...the run completes, we begin our assembly process. Again, thanks to our partners at seqWell we make use of...failed” and removed from the plate to be prepped again. These holes are then filled with other samples ...

...yourself a bit of a break before you read over it again with fresh eyes and correct it (and, believe me,...second) you won’t succeed, but you must try, try again. Now, this does not mean that you should apply for...

...microcontroller and a motor controller and made it spin again!  We’ve also learned to be resourceful and to repurpose...Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and the reagents are too ...

...that.” That was interesting. If people could be against GMOs as food but support GMOs that result in a ...business training. The money is a drop in the bucket against the funds needed to build a flower that changes...

...Mobile-CRISPRi stably integrates into the genome and is propagated during normal DNA replication and cell division...recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients with an integrated...

...detect mEPSPs, it also enabled imaging of their propagation along dendrites for the first time (Figure 3)...Ashley Waldron We recently added two antibodies against important targets for neurodegeneration research...

...over the years, several alternative antibodies against the Myc tag have been generated in an attempt to...al.’s results mean the end of 9E10’s time in the saga of the Myc tag? Hardly! 9E10’s accessibility and...

...Figure 2: Chromoprotein expression from bacteria on agar plate. Image: Liljeruhm et al., 2018. For each...for your experiment or you like how it looks for agar art, let us know! Additional resources on the ...

...negative selection marker HSV-tk is used to select against random recombinants.   Design your targeting...recombination, the negative selection marker selects against improper, random recombination into a different...

...affected by the plasmid size, nature of the insert, propagation strain, growth conditions, and the material used...Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and using ...

... used for recombinant antibody production is Komagataella phaffii (a.k.a  Pichia pastoris) because it ... density of the antibody bands can be measured against the protein standard and the concentration may ...

...such as a fluorescence based assay or running an agarose gel. In the case of plasmids, the use of RNase ... off starting from the beginning. Before trying again, revisit your purification protocol. Are any of ...

... turned to characterizing its cleavage pattern. Again, they were in for a surprise! Whereas Cas9 generates... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR. This “second chance” mechanism...

...purification with glutathione, can also protect against proteolysis. That’s one form of instability. Prokaryotes...Good for purification with glutathione; protects against proteolysis, but may reduce solubililty HA YPYDVPDYA...

...Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis Bacterial Transformation How...What is a Plasmid? How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial...

...chromosomal replication and balance population growth against sustainability. Plasmid DNA replication and regulation...proteins. In nature, such plasmids ensure their propagation by limiting their copy number and compensating...

...-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies...middle panel). Samples were also immunoblotted against vinculin as a loading control (right panel). Image...