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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 568 results for: bli

Showing: 121 - 140 of 568 results

  2. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ... and written communication skills are key to publication and acquisition of funding. If a grant committee...Understanding)   Writing Communication Publicity Consulting internships Communication...Blogging Writing Communication Networking Publicity Business competitions   Leadership ...

  3. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence...animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a...rectify this the Shu lab first improved the self-assembling capability of sfCherry approximately 20 fold ...

  4. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...funded by the public and performed by researchers who received their educations from public schools and ... BSc in Anatomy, the University of Otago had established the Centre for Science communication and I was...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...directly in DNA assembly will still yield scarless assemblies. Overview of NEBuilder HiFi DNA assembly method...simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the desired mutations... of these techniques require more complex DNA assemblies and improvements in these methodologies offer...

  6. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...libraries is well suited to large-scale de novo assemblies of short constructs like plasmids. The plexWell..., this has resulted in high quality NGS-based assemblies for over 3000 plasmids in the Addgene collection...of Toronto. He is an author of 20+ scientific publications and patents.   ...

  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...collaborative writing and this recent article published in PLOS Computational Biology (Frassl et al., ...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...

  8. How Addgene Creates Content

    Type
    Blog Post
    ... the example of our Antibody Guide, which was published alongside our new antibody services in the spring...through an editing process and final check before publication.   Do research! Nearly everything we write entails...final review to catch those pesky typos, and hit publish. Making content is an iterative process. We are...

  9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ... now. Enter the SAM pooled library In a paper published in Nature in December, Konermann and her colleagues...Zhang lab for GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout screening). As the Zhang...pooled CRISPR libraries, also available at Addgene, enabling systematic investigation of the cellular consequences...

  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...the most similar to yours. Take a look at the publications cited on the manufacturer’s website, which may...highlights a variation of your application, a publication successfully used it in a similar way as you ...safer bet than one that has never been used in a publication. Talk with your peers. If someone in your field...

  12. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Albert Einstein College of Medicine has recently published techniques to follow translation in living single...presented a novel technology, back then still unpublished, that allowed the visualization of specific de...combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for...

  13. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...Addgene blogpost and more recently in a paper published in F1000Research on November 2016. Due to the ...were at institutions where no data had been made publicly available (below). However, on November 22nd a...compelled at the state level, in the case of some public institutions) to cancel plans to raise salaries...

  15. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation...

  16. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog and is part...is science’s way of validating work prior to publication, it doesn’t mean that there are no mistakes or...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...

  17. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...and develop novel technologies! If data from a publication or preliminary data suggests the feasibility ...will empower the world” tone that differs from publication writing and may be challenging to master at first...

  18. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ... in situ hybridization experiments. A recent publication [1] from the Sánchez lab describes the plasmids...the deposit) are described in the Sánchez lab publication [1] so that the students performing these experiments..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...

  19. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...significant clinical efficacy against a form of blindness known as Leber’s congenital amurosis (LCA). This...RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until 2008. ...decline in vision with gene therapy in childhood blindness." New England Journal of Medicine 372.20 (2015...

  20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017... that contain synthesized regions that have no publicly available reference sequence or plasmids containing...these plasmids are returned as one or two partial assemblies. If we obtain an NGS result that is useful, but...
Showing: 121 - 140 of 568 results