...practicing and getting familiar with the process. Bonus points for being able to just look at a paragraph...

...using heat and/or chemicals to break some of the bonds keeping the protein folded, changing it from its...

...RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker, Thy1.1,...

...pBLADE-mCherry: This construct, built from the pBAD33 backbone, contains BLADE, the PBAD promoter, and a mCherry...

...MCS)), but do not cut elsewhere on the plasmid Bonus: It is helpful to choose restriction enzymes that...

...acquire the data you need on the new instrumentation. Bonus reasons Visiting gives you access to possible new...

...attention and answer my key questions. As an added bonus, I also got the opportunity to present what I learned...

...diverse perspectives. Everyone should read this book! Bonus, Saini's following book Superior: The Return of ...

...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

...cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the segments of mRNA to which...

...domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment (PDGFR ...

...helped tomorrow. Our activity is non-profit and pro bono. If this sounds appealing, join us and help make...

...human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019....

...Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 ...Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs ...library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs...genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting...VP64) Set A Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,901 genes and containing...

...communication skillsClear communication is the backbone of effective management. Miscommunication often...

...plasmids for their production. As such, there are bonus components to these vectors that aren’t reflected...

...-1a promoter. It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with...

...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

...Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein Tags  ...

... engineered AAV are presently built on an AAV9 backbone and so will very likely cross-react with each ...