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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 376 results for: bre

Showing: 121 - 140 of 376 results

  2. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ...plasmids through this podcast segment. Below, we break down each of the Hot Plasmids segments into its ...

  4. A Scientist's Guide to Ebola

    Type
    Blog Post
    ... five strains of Ebola representing the latest outbreak and earlier ones, along with links to the relevant...content. -And the latest updates on the ongoing outbreak from the CDC and WHO. - For anyone interested ...

  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...most CRISPR systems and generates double strange breaks at target sites 5' of the 5'NGG3' PAM sequence ...which nicks DNA rather than creating double stand breaks and may better facilitate homology directed repair...

  6. Reflections and Looking Ahead

    Type
    Blog Post
    ...empowering researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into...shared, every collaboration formed, and every breakthrough enabled brings us closer to making an impact...

  7. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly from customers...favorite ways to share science. Image credit: Judy Breck, via flickr  ...

  8. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...accomplishing it all. How do we encourage people to take a break from their everyday tasks and make time for special...However, I think it’s just as important to take a break from work to share time with the people around us...

  9. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...yeast research, a few of those have been trips to breweries who happily exchange our expertise with lots of...the lab, it is important to take the occasional breather and acknowledge the team’s personal and professional...

  10. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...this system, Cas9 generates a double-stranded DNA break at the genomic loci of interest and on either side...complementary to either side of the double-stranded break in the genomic loci of interest. As the universal...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase...

  13. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...Conveniently, the process doesn’t cause double-stranded breaks and can deliver large sequences and even entire...allowed certain mutated phages to outcompete their brethren, and the researchers isolated these more efficient...

  15. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the cleaved... of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning: 1. Create...

  16. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...research studies highlight the importance of taking a break and incorporating EAs into your routine.  Getting...learn new skills, while also getting a much-needed break from your lab bench.  Want further information ...

  17. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...it’s acceptable if it’s in the name of beer and bread. The following table lists some of the biological...end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR)....

  19. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... during the day is a great way to take a mental break, while meditating right before bed will help you...it. Plan defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique...

  20. Science Career Options

    Type
    Blog Post
    ...roles, including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...
Showing: 121 - 140 of 376 results