Multiplex Genome Editing with CRISPR-Cpf1
Type
Blog Post
... needing to work with a transfectable cell line.
2) They require larger expression vectors which are ...are also larger than their Cpf1 counterparts.
Table 2. Cas9 Multiplexing Options
Multiplexing Method... Cpf1 expression vector. See the graph in figure 2 for a comparison of editing frequency resulting from...Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548.
2. Zetsche, B., Gootenberg, J., Abudayyeh, O., Slaymaker...Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell,163(3), 759-771. PubMed PMID... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...Resources
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