...Often, the size of the plasmid insert and vector backbone are known and thus this technique can be quickly...buffer Verifying Total Plasmid Size -OR- Insert and Backbone Size The simplest form of diagnostic digest is...the expected size, or that it is composed of a backbone and insert of expected sizes. This is frequently...in two bands; the 1200bp insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting...know that you have a 1,200bp insert in a 5,000bp backbone, but there are many plasmids out there that, when...very unique pattern that will distinguish one 5kb backbone with a 1.2kb insert from all others. When choosing...orientation the insert is ligated into the vector backbone and therefore the only thing left to do is identify...
