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We narrowed to 274 results for: crispr cas9 plasmids

Showing: 121 - 140 of 274 results

  1. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ...Tools Read other CRISPR blog posts Find CRISPR Plasmids at Addgene  ...Find more CRISPR Resources at Addgene: Read our CRISPRGuide Get practical help from our CRISPR Protocols...Steensel. Easy quantification of template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume... eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post here. Dealing with low editing...version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9-HF1, eSpCas9...gRNA and introduced it into your target cells with Cas9. Hooray! Now it’s time to make sure your genome ... product of both an unedited population and your Cas9-targeted cells. Simply upload both trace files to...

  2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post
    ...speaking with us! Find the Plasmids: Plasmids for multi-input CRISPR/Cas9 genetic circuits References... future. Building circuits from CRISPRs Addgene: What can CRISPR do for synthetic biology? Voigt: The...from being used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth...Nielsen describe what’s possible when multi-input CRISPR/Cas genetic circuits are linked to the regulatory...about this collision that’s taking place between CRISPR technology and synthetic biology, the tools he’...almost limitless number of DNA binding proteins with Cas9 guided by different promoters. The challenge in ... proteins that don’t interfere with each other. CRISPR is so orthogonal and programmable that you can ...

  3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post
    .... pyogenes Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9 (PAM: NG) to...Addgene.org Get the basics of CRISPR in our CRISPR guide Find all plasmids for base editing in our collection...another evolved base editor: ABE8e Read about xCas9, a CRISPR variant with PAM flexibility Resources on ...constructs using a catalytically “dead” S. pyogenes Cas9 (dC9-ABE8.x-m/d). The dC9-ABE8.x-m/d constructs ...frequency more than 90% compared to ABE7.10. Choosing Cas9 proteins to increase the targeting scope of adenine... base editors The most commonly used S. pyogenes Cas9 requires the target site in the genomic DNA to contain... to create NG-ABE8.xm/d and the S. aureus Cas9 (PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these variants...

  4. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    Blog Post
    ...our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of the CRISPR." The newest genome...distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,000 labs around the world. Thank you! It's been ...cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are...Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...

  5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    Blog Post
    ...Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system to...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... our hot plasmid articles from April 2018. If you'd ever like to write about a recent plasmid deposit ...Inducible Recruitment for Epigenome editing by Cas9 (FIRE–Cas9), combines these approaches to enable rapid...epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found here. Braun et al. Nat Communications...epigenetic modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules...

  6. It’s CRISPR Week at Addgene!

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    Blog Post
    ... received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that...using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...too! The CRISPR field is always changing so we’ve been busy updating our CRISPR guide. Our CRISPR guide ...for planning your CRISPR experiment. For a quick guide to CRISPR, download our CRISPR Cheat Sheet....you use CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If...That’s a lot of CRISPR! As we’ve shared these tools over the years, the amount of CRISPR educational resources...about CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start with a CRISPR overview...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post
    ...experience helping scientists share plasmids, especially those of the CRISPR variety (see slides from my talk...complete without CRISPR Michael Terns from of the University of Georgia studies the CRISPR/Cas system as ... proposes using bacterial phage vectors with CRISPR/cas9 sensitizing gene cassettes. Thse vectors are ....  There are many clever ways labs are using CRISPR/cas9 and more are being reported. Zhou Songyang from... tetracycline cas9 inducible knock-out system. His lab is also exploring the use of cas9 proteins from...is in one of the top 10 countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic...introduction to the repeated and distinct aspects of the CRISPR regions of the prokaryotic genome. Then he desribed...

  8. Site Directed Mutagenesis by PCR

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    Blog Post
    ...mutagenesis of Plasmids. A) Generation of mutagenized plasmids. PCR primers (green) amplify the plasmid template...Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to Generate Specific Genomic...occuring SNPs in a plasmid context). The technique is also highly relevant in this age of CRISPR; site-directed... through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed... the template should be resistant to Cas9 cleavage. If a plasmid contains the template, site-directed ...critical for Cas9 cleavage), thereby rendering the resulting construct resistant to Cas9 induced cleavage...with the nuclease-resistant nicked plasmid (the PCR product). Plasmids are isolated from the resulting colonies...

  9. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post
    ...MECP2 protein, showing mutations corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from...from Le et al., 2019. Gene editing via CRISPR/Cas9 and RNA-mediated correction of MECP2 mutant alleles...disease. Klaus Rajewsky’s group recently used the CRISPR/Cas9 system to both precisely insert the MECP2-R270X...Joung, PhD, and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for precise correction of Rett syndrome...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...Rajewsky K, Chu VT (2019) Efficient and Precise CRISPR/Cas9-Mediated MECP2 Modifications in Human-Induced...to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome...

  10. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post
    ...this CRISPR plasmid can be created in a single tube. You can also find additional SapTrap plasmids used...more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog posts Resources on...your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap ...SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR targeting vectors that each...Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans."...Bargmann’s keynote speech and in the workshops on CRISPR techniques and new tools for conditional expression...discussed at the meeting, in addition to useful plasmids and toolkits available at Addgene.  C. elegans...

  11. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ...on Your CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled ...by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following...2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https...Incidence of Off-Target Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human Stem Cell Clones Detected...How to Conduct Genome-Wide CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org...transcription – CRISPRa (activation) and CRISPRi (inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed... capacity of a locus. When using S. pyogenes Cas9 (SpCas9), potential target sites are both [5’-20nt-NGG...

  12. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post
    ...Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR guide ...genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma... of these visualization tools is CRISPR-Sirius, the brightest CRISPR-based tool to date for visualizing...  Developing CRISPR-Sirius Recently, Hanhui Ma and the Thoru Pederson lab developed CRISPR-Sirius (Ma et...Why try CRISPR-Sirius? With all the tools out there to visualize genomic loci, what makes CRISPR-Sirius... Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR blog posts Resources...fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific...

  13. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    .... New CRISPR plasmids available! Interested in purifying Cas9? Check out pET-28b-Cas9-His from Alex...information on these new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage...CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download...key step for CRISPR/Cas9 gene editing. Dr. Masahito Ikawa has created a GFP reporter plasmid for scientists...purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from Scot...Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus.... 2014..) Our CRISPR-Cas collection of plasmids updates frequently, so visit our CRISPR pages often to...

  14. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources page...yeast CRISPRa plasmids here Reduce the scale of your CRISPR screens by using this lentiviral CRISPR human...use in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items from ...sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To find...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox with their...

  15. CRISPR Cheat Sheet

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    Blog Post
    ...RNA sequences. Cas9 is a very commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene ...Applications of CRISPR: Cut: CRISPR can be used to cut the DNA sequence. With a repair template, CRISPR can be...Jennifer Tsang. We have some crisp new CRISPR material for you!  The CRISPR field moves fast. That’s why we’...Addgene’s CRISPR 101 eBook. This new edition of the eBook includes the details on advances in CRISPR including... Prime editing Anti-CRISPRs … you name it! So get excited and download the CRISPR eBook today! P.S - ...refresher before you dive into the CRISPR eBook, check out the CRISPR Cheat Sheet. Download the PDF using...same information. Download a PDF of CRISPR Cheat Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced...

  16. Design Tips for Prime Editing

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    Blog Post
    ...Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by function...editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase and a prime editing...editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer binding site ...., 2019), disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure...pegRNAs that use other bases in this location bind to Cas9 with canonical sgRNA structure (right). Created ...along with your intended edit. This prevents the Cas9 nickase from re-binding and nicking the newly synthesized...Zhang, F., & Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, ...

  17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post
    ...by Type II systems (such as CRISPR-Cas9). Unlike in regular Type I CRISPR-Cas systems, however, Cascade... involving two-plasmid or single-plasmid systems (Vo et al., 2020). In the two-plasmid system, an effector...Resources on Addgene.org Visit the CRISPR guide Find CRISPR plasmids for bacteria ...group, and CAST (CRISPR-associated Transposase) by the Zhang group. Mechanism of CRISPR-transposons One...effector plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter...Type I-F INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow and technical...) into the CRISPR array Clone desired DNA cargo into the mini-transposon Transform plasmid(s) into cells...

  18. Hot Plasmids Spring 2024

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...pooled CRISPR screens By Emily Bentley Building on their CROPseq method for optical pooled CRISPR screening...has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton et...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...Huntington’s Disease, including plasmids, antibodies, viral vector preps, CRISPR tools, and more.  ...for a range of applications, including CRISPR activation by dCas9-VPR, prime editing, and delivery of disease-relevant...Multiplexed perturbation and decoding in pooled CRISPR screens Improved prime editor PE7 Antibodies to...

  19. Hot Plasmids - October 2020

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources page....2020.107804 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To find... Get the plasmids at Addgene The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library that...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... Get the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease ...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...

  20. Making CRISPR Plasmids Using Fragmid

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    Blog Post
    ....) CRISPR enzyme (Cas9 [sp], Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa, CRISPRi...resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: Golden Gate Cloning...restriction digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just ...correctly banded clones for plasmid sequencing and found 98% of the plasmids were perfectly correct. Because... Mammalian Plasmid pRDA_791 (contains only orio and ampR) Drosophilia Plasmid pRDB_051 (for...the assembled plasmid. It shows you each component, with the ID of the component plasmid, component name...the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating...
Showing: 121 - 140 of 274 results