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We narrowed to 369 results for: epor

Showing: 121 - 140 of 369 results
  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren ...optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers...interneurons in mice. A) AAV expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer...
  2. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter...into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs...Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer Science and Business Media...
  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...ligands for dopamine D2/D3 receptors. Scientific Reports, 10(1), 21842. https://doi.org/10.1038/s41598-020...fluorescence-based ligand-binding assay. Scientific Reports, 6, 25769. https://doi.org/10.1038/srep25769 Grimm...mitochondrial dynamics in mammalian cells. Biophysics Reports, 3(4–6), 64–72. https://doi.org/10.1007/s41048-...
  4. CRISPR Plasmids - Tagging

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    Collection
    ...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...
  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution... iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28...
  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using...TRIBE is the low input required - McMahon et al., report using as few as 150 fly neurons as their starting...delivery of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the...
  7. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...Neuroscience Community blog to read reports from bloggers at the meeting – my report is currently in the works....
  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...
  9. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...
  10. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...using the BE4max architecture. The new editors reported in this study have ~10-100-fold lower average ... gRNA multiplex. Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation...
  11. The Pleiades Promoter Project

    Type
    Collection
    ...MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A pEMS1142 EGFP/NLS Ple3 ADORA2A pEMS1143...
  12. Caltech Systemic Capsids

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    Collection
    ...tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (...
  13. An Addgene Summer Internship

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    Blog Post
    ...capstone project report and presentation, during the final week at Addgene. This report shared my work ...
  14. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...
  15. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...
  16. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...did you use? For animal subjects it is helpful to report the genetic background and catalog number or RRID...take negative data, which is equally important to report. We recently published a paper in Frontiers in ...
Showing: 121 - 140 of 369 results