We narrowed to 217 results for: grna

CRISPR plasmids that have been designed for use in yeast.

CRISPR plasmids that have been designed for use in Drosophila and other insects.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

CRISPR expression plasmids that have been designed for use in plants.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.

CRISPR plasmids that have been designed for use in bacteria.

CRISPR nickase plasmids for the generation of single-strand breaks.

CRISPR plasmids that have been designed for use in mammalian systems.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

CRISPR plasmids that create double strand breaks in DNA.

Find plasmids for CRISPR-Cas and Cascade for large genomic deletions.

CRISPR plasmids available at Addgene. Search by species and CRISPR function.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

The Framework for In vivo T cell Screens (FITS) allows for in vivo CRISPR screening in T cells. Learn more about FITS in our blog post.

An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering tool.

CRISPR plasmids for transcriptional repression of target genes.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.