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We narrowed to 244 results for: grna

Showing: 121 - 140 of 244 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL...containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The...Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor CEBPB Human FLAG...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...#79888) , which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid...

  2. CRISPR Plasmids - Parasites

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic parasites...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the... - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second...small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all of an AAV’s ~4.5 kB packaging...

  6. CRISPR Guide

    Type
    Collection
    ...optimized gRNA for your application (see: gRNA Design Software ). In addition to an optimized gRNA design... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide... between the gRNA scaffold and surface-exposed, positively-charged grooves on Cas9. gRNA binding induces...

  7. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers...it is easy to implement due to the simplicity of gRNA design, programmable for different genomic loci,...efficiently visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  8. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

  9. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...Cas9. With many PAMs available, a large number of gRNAs could be screened for the most efficient editing...different mutations in different patients. While these gRNAs would still need to be optimized and assessed for...the best when paired with the highest-efficiency sgRNA. The PAM sequence near this site is AGC, so in a...

  10. CRISPR Plasmids and Resources

    Type
    Collection
    ...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment...experiment based on a variety of factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...

  11. Validated gRNA Sequences

    Type
    Collection
    ... Genome Engineering CRISPR Validated gRNA Sequence Datatable Validated gRNA Target...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences that have...Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...indicate the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable Target Gene...details and outcomes. Submit Validated gRNA Sequences Validated gRNA sequences can be added to this table...validated gRNA sequences. Genome...

  12. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  13. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...PEmax to enhance pegRNA stability epegRNA — additional protection added to 3’ tail of pegRNA to prevent RNA...nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI ...BbsI for sgRNAs; BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3 pegRNA BsaI + HindIII No Caixia...

  14. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...genomic DNA bound by the gRNA. An epitope tag or tags can be fused to dCas9 or gRNA for efficient purification...of any genomic sequence specified by a particular gRNA. In the enChIP (engineered DNA-binding molecule-...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  15. Editor's Choice, August 2016

    Type
    Blog Post
    ...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries ...Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is a useful...experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic ...thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening...libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...

  17. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...lab. The Bikard library contains ~93,000 unique gRNAs that target random positions along the E. coli MG1655...targeting requires two sgRNAS: one that targets the knock-in site and a universal sgRNA that targets the donor... the genomic loci of interest. As the universal sgRNA is not predicted to target sites in the human, pig...

  18. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of ...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  19. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...spacer and the remaining sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the ...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA... tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8...complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “... on the 3’ end of an RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this ...
Showing: 121 - 140 of 244 results