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We narrowed to 326 results for: iNOS

Showing: 121 - 140 of 326 results

  1. Tips for arabidopsis transformation

    Type
    Blog Post
    ...of culture you are using and whether you are inoculating from a frozen stock or a colony from an LB plate...plate. In my hands, a 5 mL starter culture inoculated from a colony on a plate will reach an optimal density...

  2. CRISPR Antimicrobials

    Type
    Blog Post
    ...Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and ...plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single base pair change...

  3. Plasmids 101: Protein tags

    Type
    Blog Post
    ...affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash for your protein...; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus, promotes...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea...formed from a maturation reaction of three specific amino acids at the center of the protein (Ser65, Tyr66...

  5. DNA Quantification

    Type
    Protocol
    ...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering Plasmid...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni ...editing than SpCas9 (Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM...

  7. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016...Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional...

  9. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections ... Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest ...

  10. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett...10.1016/j.cell.2014.09.029 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still...expression strain to make sure they are compatible. An arabinose-inducible plasmid will not express in an IPTG ...

  13. Protocol - How to Design Primers

    Type
    Protocol
    ... also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...

  14. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non...fluorescence of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from ...

  15. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known ... antigen-binding fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...palindromic and repetitive sequences, but if present, a minor extension can usually ensure that the 3’-base(s)... of the most popular approaches for introducing minor modifications to a plasmid template. Many thanks...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...rule of thumb, we avoid target sites that code for amino acids near the N’ terminus of the protein, in order.... Likewise, we avoid target sites that code for amino acids close to the C’ terminus of the protein, to...
Showing: 121 - 140 of 326 results