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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 136 results for: max

Showing: 121 - 136 of 136 results

  2. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...tract lengths seem much shorter. Q10: What is the maximum amount of DNA that can be inserted into the genome...

  4. DNA Quantification

    Type
    Protocol
    ...your substance maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is ...and most have a specific wavelength that they maximally absorb. By measuring the absorbance of a liquid...is at 260nm. Protein maximally absorbs at 280nm and the ratio of nucleic acid to protein (260/280) is generally...

  5. Adenovirus Guide

    Type
    Guide
    ...use of pAdEasy-1. Created with BioRender.com. AdMax™ AdMax™ is another well used rAdV vector production...adenoviral genes necessary for packaging. In the AdMax™ system, recombination between both plasmids occurs...the HEK293 packaging cell line, engineered for maximum adenoviral production, and is driven by use of ...

  6. CRISPR Guide

    Type
    Guide
    ... a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between.... PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson, A. R. (2013). Bacteriophage...Rollins, M. F., Hidalgo-Reyes, Y., Wiedenheft, B., Maxwell, K. L., & Davidson, A. R. (2015). Multiple mechanisms...Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring...

  8. Pouring LB Agar Plates

    Type
    Protocol
    ...and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip If you will be screening...like to make 20 plates, and our plates can hold a maximum of 10 mL, we’ll want 200 mL of media total. Importantly...

  9. Guide to Using Pooled Libraries

    Type
    Guide
    ...either case, next-generation sequencing of the maxiprep DNA is recommended to verify that the library ...process. Amplify the library (electroporation and maxiprep). If delivering as virus, make virus; this creates...

  11. Water Bath Protocol

    Type
    Protocol
    ...fill the water bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside...

  12. Pipetting Protocol

    Type
    Protocol
    ... might hold a pipette. Without moving past the maximum possible setting on the pipette, set the desired...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...but do not stick to PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM...

  15. Plan Your Experiment

    Type
    Guide
    ... it is also important to consider factors that maximize cleavage of the desired target sequence, or on-target...
Showing: 121 - 136 of 136 results