We narrowed to 275 results for: mel

1. Fluorescent Proteins 101: Fluorescent Biosensors

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   Blog Post

   ...another common class of biosensors. Blue flame “cameleon” calcium sensors created by Roger Tsien’s lab ...

2. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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   Blog Post

   ... :D) engineering E. coli to produce biofuels in Pamela Silver’s Lab (plasmid page here). Now I spend much...

3. Management for Scientists: Giving Feedback

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   Blog Post

   ...to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect...

4. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   Blog Post

   ...circuitry and synthetic biology, and I found myself extremely grateful to my colleague Jason for choosing Simon...

5. Advice for Choosing a Research Project

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   Blog Post

   ...productivity and direction of the project? What is your timeline? A typical research project can take several years...

6. The Stingy Scientist: How the Baby Gel Box Was Born

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   Blog Post

   ...Outreach Scientist, Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent...

7. Choosing a Good Mentor for Scientists

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   Blog Post

   ... Consider reading Toxic Academic Mentors by @drmellivora, an excellent blog post about workplace bullying...

8. What the HEK?

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   Blog Post

   ...viral therapies. Protein production  HEKs are extremely easy to transfect in bulk and produce recombinant...

9. Addgene Begins Distribution of Recombinant Antibodies

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   Blog Post

   ... Melina Fan...

10. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post

    ... Melina Fan...

11. Meet the New Addgene Board Members!

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    Blog Post

    ...delivery. In 2018, he interviewed Addgene co-founder Melina Fan and former Associate Director of Viral Vectors...

12. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    Blog Post

    ...unstable ground around these hot springs make them extremely dangerous, we used telescoping poles to collect...

13. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post

    ...industrial settings Tight (sometimes impossible) timelines—others are usually waiting Work is concentrated...

14. Plasmids 101: TOPO Cloning

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    Blog Post

    ... at a low concentration or you are cloning an extremely large insert. Since the standard ligation reaction...

15. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    Blog Post

    ... Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, and Nature, 1945–1990...

16. Plasmid Cloning by PCR

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    Blog Post

    ...should select an annealing temperature based on the melting temperature (Tm) of the portion of the primer that...

17. Addgene's Top 10 Blog Posts of 2015

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    Blog Post

    ...you’ve made the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation...

18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post

    ...made CRISPR/Cas9 so successful in this species, namely, the robust HDR mentioned above, an accessible ...

19. Trading Spaces - Visiting Scientist Edition

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    Blog Post

    ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...

20. K. phaffii: Rising to the Occasion

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    Blog Post

    ...Affordable to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to...