...pathophysiological complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation...renewable sensor, a bacterium that can sense contaminants and emit light as a response. To create this...fluorescent protein transcription in response to river contaminants and transform these plasmids into a bacterial...

...field-based software product manager at Oxford Nanopore Technologies. If the first thing you hear when...streamlined manner. Now, in my current role at Oxford Nanopore Technologies, I work with our customers in the...currently a Field-based Product Manager at Oxford Nanopore Technologies for the North America region. She...

...rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are used. For predominantly retrograde tracing, albumin protein labelled...PMC2600441. Foust, K.D., Nurre, E., Montgomery, C.L., Hernandez, A., Chan, C.M. and Kaspar B.K. (2009). Intravascular...2009). Comparison of transduction efficiency of recombinant AAV serotypes 1, 2, 5 and 8 in the rat nigrostriatal...motor neurons after intramuscular delivery of recombinant AAV serotype 6. Gene Ther 17, 141–146. PubMed...

...optogenetics, and chemogenetics experiments. Recombinant AAVs (rAAVs) The primary application of AAV is...the deliverable DNA) are removed, resulting in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...

...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...embryos produced were not being used to establish a pregnancy, the researchers were able to examine each individual...

... CRISPR’s therapeutic potential in mice with a dominant cataract disorder caused by a single copy of a...have experienced hiccups (either clinically or financially), and others we just don’t have the time to talk...technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to the liver...

...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...immunogenic profile. AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral... responses to adeno-associated virus and its recombinant vectors." Gene therapy 10.11 (2003): 964-976....

...nature, agriculture and bananas. You can follow her on Twitter @BananarootsBlog.   Additional Resources...engineering resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow at The Sainsbury Laboratory...

...research focuses on understanding the structural determinants of protein-DNA interactions and how these interactions...developmental delay, result in loss of function or dominant negative NFIX phenotypes. Developing a rapid ...

...virus, we will see that they are very central determinants of how AdVs behave, and in fact, of how adenoviral...from the genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the...natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral vectors all have a genomic...

...Cloin BMC, Finan K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label density...

... genes required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball...hMDH1). They then expressed the original and recombinant enzymes in bacteria, purified them to near homogeneity...complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119...

...T. R. (2015). Stability and compatibility of recombinant adeno-associated virus under conditions commonly...Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct...Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent...

...this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic gRNA, is incubated...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4...

...to aid in the expression and purification of recombinant proteins. Tags for stability and solubility ...hurdles to overcome in order to overexpress a recombinant protein? It is not generally in a cell’s best...glutathione S-transferase (GST), which can be fused to recombinant proteins for one-step purification with glutathione...

...Watch the Video! Water Baths Proper water bath maintenance and use Watch the Video! Pipetting Learn about...produce antibodies Antibody Purification Purify recombinant antibodies using Protein A or Protein G columns... Stain Determine purity and concentration of recombinant antibodies Watch the Video! Western Blot Separate...

...results in the production of air-filled protein nanostructures called gas vesicles, which scatter sound waves... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and...Maresca, D. et al. Nonlinear ultrasound imaging of nanoscale acoustic biomolecules. Appl. Phys. Lett. 110, ...

...non-specific binding or false positives.  If testing a recombinant protein, especially one with tags, be sure to...the endogenous protein, since folding of the recombinant protein may differ from the native form, which...addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...

...implemented and from that, develop better software.  Maintenance  We’re not just about developing new features...: we take time during each sprint to do some maintenance work on our code. One way to look at this is ...continuing to add new features.  Another area of maintenance is agreeing to the names of the things in our...

..., Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose or magnetic...their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was developed, which can...on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...