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We narrowed to 388 results for: nans

Showing: 121 - 140 of 388 results

  1. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...conversion is a unique characteristic of recombinant antibodies. Recombinant antibodies are plasmid-based and ...is routinely used to increase the utility of recombinant antibody tools, the resulting chimera may have...

  2. What the HEK?

    Type
    Blog Post
    ...produce recombinant proteins at high levels. In a case where clinical applications of recombinant proteins...., HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol...

  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...pathophysiological complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation...renewable sensor, a bacterium that can sense contaminants and emit light as a response. To create this...fluorescent protein transcription in response to river contaminants and transform these plasmids into a bacterial...

  4. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

  5. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are used. For predominantly retrograde tracing, albumin protein labelled...PMC2600441. Foust, K.D., Nurre, E., Montgomery, C.L., Hernandez, A., Chan, C.M. and Kaspar B.K. (2009). Intravascular...2009). Comparison of transduction efficiency of recombinant AAV serotypes 1, 2, 5 and 8 in the rat nigrostriatal...motor neurons after intramuscular delivery of recombinant AAV serotype 6. Gene Ther 17, 141–146. PubMed...

  6. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...field-based software product manager at Oxford Nanopore Technologies. If the first thing you hear when...streamlined manner. Now, in my current role at Oxford Nanopore Technologies, I work with our customers in the...currently a Field-based Product Manager at Oxford Nanopore Technologies for the North America region. She...

  7. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Watch the Video! Water Baths Proper water bath maintenance and use Watch the Video! Pipetting Learn about...produce antibodies Antibody Purification Purify recombinant antibodies using Protein A or Protein G columns... Stain Determine purity and concentration of recombinant antibodies Watch the Video! Western Blot Separate...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...optogenetics, and chemogenetics experiments. Recombinant AAVs (rAAVs) The primary application of AAV is...the deliverable DNA) are removed, resulting in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...

  9. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...embryos produced were not being used to establish a pregnancy, the researchers were able to examine each individual...

  11. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...immunogenic profile. AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral... responses to adeno-associated virus and its recombinant vectors." Gene therapy 10.11 (2003): 964-976....

  12. CRISPR in the Clinic

    Type
    Blog Post
    ... CRISPR’s therapeutic potential in mice with a dominant cataract disorder caused by a single copy of a...have experienced hiccups (either clinically or financially), and others we just don’t have the time to talk...technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to the liver...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...T. R. (2015). Stability and compatibility of recombinant adeno-associated virus under conditions commonly...Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct...Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent...

  15. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic gRNA, is incubated...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    ...virus, we will see that they are very central determinants of how AdVs behave, and in fact, of how adenoviral...from the genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the...natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral vectors all have a genomic...

  17. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Cloin BMC, Finan K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label density...

  18. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... genes required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball...hMDH1). They then expressed the original and recombinant enzymes in bacteria, purified them to near homogeneity...complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119...

  19. Plasmids 101: Protein tags

    Type
    Blog Post
    ...to aid in the expression and purification of recombinant proteins. Tags for stability and solubility ...hurdles to overcome in order to overexpress a recombinant protein? It is not generally in a cell’s best...glutathione S-transferase (GST), which can be fused to recombinant proteins for one-step purification with glutathione...

  20. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...implemented and from that, develop better software.  Maintenance  We’re not just about developing new features...: we take time during each sprint to do some maintenance work on our code. One way to look at this is ...continuing to add new features.  Another area of maintenance is agreeing to the names of the things in our...
Showing: 121 - 140 of 388 results