...to see these populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this ...is define a point where the peak is sufficiently narrow to count as a “true” signal.  Figure 8: ...

...introductory teaching and research environments (Idalia & Bernardo, 2017).  Escherichia coli. Image from NIAID...doi.org/10.1242/dmm.002733 Idalia, V.-M. N., & Bernardo, F. (2017). Escherichia coli as a Model Organism...

...BioRender.com   The first couple of steps are preliminary steps that are not exclusive to IF, but they ... epitope, and sample type (Im, et al. 2019). To narrow down which specific methods to use, start by reading...

...to study the effects of protein expression in stationary phase. In these cases you may want to control...

... 84,963 Human CRISPR Knockout Pooled Library (Gattinara) 136986 Knockout Human Doench 3rd 2 40,964 Ingolia...

...with early career science policy groups, attend webinars, and learn more about the field.  Read the CCST...accelerator for some hands-on guidance.   Check out my webinar via the NC Science Policy student group SciPolPack...

...fellowship grants) A project with sufficient preliminary data to establish your own lab and get an independent...their research. They dread giving a departmental seminar or a joint lab meeting. I've been told that PFs...

...purchased from various other suppliers with a typical turnaround time of 5-7 days. The crRNA and tracrRNA are ...from commercial suppliers of CRISPR reagents (turnaround time 5-7 days); because the tracrRNA is the same...

...during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute...in plasmid purification kits Kit Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced...

... studies with clinical endpoints. In the first scenario, robust and efficient generation of iPSCs is the...better suited for these situations. The second scenario requires higher levels of safety and as little...

...onto other proteins to confer functionality. In Narayan et al., they used this now classic technique to... of their useful reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671...

... assay type) and search again. After filtering, narrow down further by company, price, and other factors...

...chloride pump from Haloarcula (Halobacterium) salinarum (strain Shark), used to silence neuronal activity...2012 Prog Brain Res. 196: 1-28. PMID 22341318 Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash ...DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K. 2011. Principles for...

...CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation...

...and Deeper” by Jorge Cham   2)   “Boxplot” by Maki Naro   3)   “Bird and Moon” by Rosemary Mosco   4)   ...

...filters on the right-hand side of the search page to narrow your keyword search to one of these organisms. ...

...other video series.   And if you haven’t seen our webinar “Navigating an Expanding Landscape for Engineered...

...consideration, and with much debate, we have finally narrowed it down to three choices.   Fig. 1: Lin...

...graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded, Ben and Niroshi, were finishing...

...serotype pAAV-mDlx-NLS-mRuby2  AAV PHP.eB Controls Gradinaru New serotype pAAV-CaMKIIa-mCherry  AAV PHP.eB...