...sharing Addgene is all about helping scientists share between labs, and people work hard at Addgene to make...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...sports, movies, and just about anything. As our organization grew, we continued this tradition. We now have...as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can only provide...delicious) team building activity that is easy to organize. We’ll dedicate a post to these competitions in... started Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams...

...author do not represent a formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister...rights (2). Less formally, governments are the organizations that make and enforce the rules of the lands...climate change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines... while their website lists over 200 partner organizations, and 514 satellite marches are scheduled to ...author do not represent a formal stance taken by Addgene or its staff. Many thanks to our guest blogger,...Chronicle of Higher Education: “March for Science” Organizer Says It’s About the Public, Not the Scientists...

...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed ...systems The ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015). “A Scalable...26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and ...57. PMID: 26701308. Additional Resources on the Addgene Blog Brush up on Homology Directed Repair and ...

...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how... happy to have the support of the following organizations to share materials more broadly to accelerate...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists...to industry? Over the past 8 years, several organizations have made their plasmids available beyond academia...

...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn How...and some of the great biological societies that Addgene has had the opportunity to work with at Freshers...support each other and provide advice. The society organizes numerous recreational and educational events throughout...outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to see these biological ...society or event which would benefit from some Addgene support? Please get in touch, as we aim to support... How to Get Involved in a Profession Organization ...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...

...that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe that...send directly from a mutual connection. If the organization is small, any person at the company might be...be able to help you. If the organization is large, you will need to be more directed and target someone....  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series.    ...

... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...many cases, however, a GFP-tagged cell line or organism has already been made, and it would be expensive...already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...

...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...making this transition. In this installment of the Addgene career guide, we’ll think about what causes this...job candidate for a senior scientist position at Addgene, the skeptical applicant asked if she would partake...of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list of things... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is always ...someone who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly...

... a Professional Organization Resources on Addgene.org Check out Career's at Addgene ... the Management Team that steers the organization. As an Addgene vet familiar with the expertise and skills...joined Addgene’s Green Team, a like-minded group of co-workers who want to make our organization as environmentally...unless we manage to make Addgene the most environmentally friendly organization on the planet, in which...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting... by some colleagues as the “cross-team guy” at Addgene, an unofficial title that I happily embrace (and...possibly understand every nuance of how kits work at Addgene, but with the help of these people and others, ...

...Browse Addgene's collection of luciferase plasmids for assays of transcriptional regulation and bioluminescent... bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects... The most common types of luciferase found in Addgene’s collection are Firefly luciferase (Fluc), Renilla...interest before or after luciferase . In addition, Addgene has many luciferase expression constructs available...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...expert to see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the...applications. For more information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR... CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models: Mammalian systems...

...facing a financial crisis. As a nonprofit organization, Addgene charges enough for our services to run our...funding organizations to request support for our operations until our normal work resumed. Addgene distributes...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small... all over.” And we sent the Addgenies home.  At the same time, as Addgene started dealing with this new...resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure...science sharing as we can get. The effects of Addgene’s brief shut-down and the subsequent fluctuations...don’t have space in this blog to call out every Addgene Team because they’re all doing amazing work during...

... Stopwatch! Any organization that deposits with and/or requests materials from Addgene can qualify. We...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material ...question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer...arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last ... for depositor and requestor alike. That’s why Addgene works closely with TTOs to help protect our depositors...

...FOP until a cure is available. Addgene and AJ's Avengers This year Addgene, where my wife Lianna has made...their plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers... Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money...helping us fight against FOP in two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of...We will be collecting donations for IFOPA, an organization dedicated to raising funds and awareness of ...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...

...and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene Browse all AAV vectors...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...University. Find the Spaghetti Monster probes at Addgene Uses of smFPs 1. Multi-channel connectomic tracers...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...PubMed PMID: 18057027. Additional Resources on the Addgene Blog Rabies and neuronal tracing Retrograde AAV...

...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...

...below and an Addgenie will help you find what you need.   More Plasmid Resources from Addgene: Plasmids... is which vector will best suit your needs? At Addgene, we have a vast collection of empty backbones that...your expression system or environment. The host organism will determine the type of vector that you will...your plasmid has been incorporated into the host organism, usually achieved with the proper selection marker... of the specific mutations. For these purposes Addgene has a large collection of yeast expression vectors...Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other Plasmid Protocols List of common epitope ...

...skills”. Leadership is prized at all levels of an organization and is also one of the most common criteria ...work alone much of the time, depending on the organization they lead. Effective leaders rarely give orders...series of blog posts in one pdf - download the Addgene Management for Scientists Ebook.  Fire, Snowball...

...Dimers and Multimers" post. It's also been re-organized and updated with a fresh new look.  We think this...plasmids, they don’t go to Wikipedia; they go to Addgene.”-Ryan Mehl, Professor, Oregon State University...

...grow liver stem cells and liver-like mini-organs or organoids, neither of these can substitute for a liver...career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...talk preparation: Define your research vision. Organize your vision into a chalk talk format. Practice...questions from open ended screens and data collection. Organize your research vision into the chalk talk format...outdoor photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check...