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We narrowed to 416 results for: plasmids 101

Showing: 121 - 140 of 416 results

  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ....  Table 1: Monomeric FP Timers and Associated Plasmids from Subach et al. Protein Excitation (nm) ...) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red...

  2. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment...Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W., Shah, S. S...

  3. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...paper in Nature Communications Find the Cas12b plasmids  Plant base editing with a Cas9-adenosine deaminase...wheat. Read the paper in Genome Biology Find plasmids containing these base editors Spatially and ...Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA cloning (STAgR) String ...the paper in PLoS One Find the STAgR cloning plasmids Mobile-CRISPRi allows CRISPRi experiments in...

  5. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN (1981) “Western...Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton ...

  6. Chemogenetics Plasmids

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    Collection
    ...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may also like... Chemogenetics Guide Viral...Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics Chemogenetics plasmids encode genetically ...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for chemogenetic experiments, including popular DREADDs and PSAMs. Plasmid... Plasmid Collections ...

  8. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...Additional resources on the Addgene blog CRISPR 101: Making a Knock-In Cell Line Using CRISPR-Cas9 to...resources on Addgene.org CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development ...

  9. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Communications Biology Find the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE...paper in mSphere Find the dCas9 and dCas9-Mxi1 plasmids If you have a new CRISPR tool you’ve recently...

  11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...insect cells, they are most often expressed from a plasmid in bacteria (Ahmed et al., 2012). One challenge...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

  12. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible...light-inducible Cas9 activity? Check out these these plasmids Catch Up on Your CRISPR Background with Our Guide Pages...CRISPR-Cas9. Cell 170:1224-1233.e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang ...Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, Pinilla-Redondo R...Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes...-Cas9. Cell 167:1829-1838.e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu J-J, Bray...

  13. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang...RESCUE. Read the paper in Science Find RESCUE plasmids If you have a new CRISPR tool you’ve recently...

  14. To Each HIS Own

    Type
    Blog Post
    ...on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to Antibodies... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...assays in the lab (for a refresher see Antibodies 101: Introduction to Antibodies.) Unfortunately, for ...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Blog CRISPR 101: Homology-Directed Repair CRISPR 101: Cytosine Transversion Editors CRISPR 101: Any Base...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope...you can learn more about in this blog post: CRISPR 101: RNA Editing with Cas13. Finally, the development... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...

  17. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...antibody-based protocols - but thankfully this is an Antibody 101 post, so we'll keep it simple. If you follow the ...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

  18. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...vector sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli and human...expression. Read the paper in Nature Find the CasX plasmids Drug inducible transcriptional activation and... Acids Research Find the drug inducible Cas9 plasmids E. coli genome-wide inhibition library In the...

  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped virus...
Showing: 121 - 140 of 416 results