...now available in lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA...the Addgene Advisory Board) says: "When you're setting up to do a genetic screen, you need to focus on...

...instructions use NEB buffers. Nonetheless, if you are setting up a double digest with NEB enzymes, the app is...of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence... can’t guarantee every reagent in your chemical closet is in this app. However, if you get quizzed randomly...

...communication to another person.  In an interview setting, this means persuading an employer that your past... to ask and answer these questions for a diverse set of audiences.  Every scientist needs to be able to...skills in science communication will be a positive asset throughout your career? 1. Make scicomm practice...of this as a jumpstart or a refresher for your skillset rather than a permanent fix. Treat every interaction...

... lacZ in response to green light. BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids...fluorescent protein variant (CyPet) found in pCyPet-His . pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger...

...Conformationally destabilized Firefly luciferase mutants : A set of three sensors that are increasingly structurally...biosensors, or optogenetic tools . pcDNA3.1+-pHlash , pRSETb-pHlash : A pH sensor created by fusing a bright...

...invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages infecting ...

...Targeted gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested.... SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized SaCas9; SaCas9...

...DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, ...

...fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash...obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...the MIT Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on ...model systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used...now available at Addgene, so on your marks...get set...RUSH. Boncompain et al., Nat Methods 2012. Mar...; the Cell-free expression test kit represents a set of 88 clones in a pCellFree vector that enables protein...

... based on the identity and order of a particular set of inputs, may be used to better understand and engineer...on the state cannot be undone and also limit the set of possible downstream states that can be achieved... register to record all permuted substrings of a set of chemicals. Specific examples of arrangements that...possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that uniquely...

...simply pause and take a deep breath before the panic sets in. Most of the time, that’s enough–but if it’s ... lab meetings, you’ve developed a valuable skill set that will be useful in nearly any other job you may...

... many of the same elements as a STAR answer: a setting, a conflict, and a resolution. Stories usually ...interviewers will not have the same experiences as you, so set them up for success by giving them sufficient background...

...variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting that researchers...being able to recognize it as a part of your skill set later on. If you are new to research and the lab,... define this skillset. After sifting through dozens of career advice articles, I settled on defining problem...qualified to be an astronaut. But, knowing that this skillset is a part of your toolkit is important. Paired...

...Whenever an order is placed on the Addgene website, it sets off a chain reaction of events throughout all of...dealing with the samples to newer lab members. We can set ourselves up for success by giving explicit instructions...one of these steps can fail but, in our current setup, there are no explicit instructions on what to do...but critical logic. Some steps fail “hard”, they reset the whole procedure. Other steps fail “soft” they... and capital cost of getting up to speed with a setup is almost always worth it. Because labels are applied...

...or circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden...unpredictable cases, MoClo provides two answers: first, sets of parts with characterized behaviors are available...

...paper from Ed Callaway’s lab (Fischer, KB. 2019) set-out to systematically test the potential causes of...expression in vivo.  Since DIO/FLEx vectors contain two sets of homologous recombination sites, they hypothesized...should now be able to make smaller adjustments to a set of injections into your animal model. For example...of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing (NGS) (Guerin...

...may generate irreproducible data in a research setting, but these events could have disastrous health ...criteria are not exhaustive. Mismatch tolerance settings are often limited to off-target sites of <4 bp...clinical approval. It is key to maintain a consistent set of standards as the field seeks to generate reproducible...streptavidin and next-generation sequencing (BLESS) (Crosetto et al. 2013). GUIDE-seq and BLESS detect double-stranded...25503383. PubMed Central PMCID: PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution DNA double-strand...

...CRISPR knockout screens have been used in several settings, and are fantastic tools for unbiased discovery...library before using them to understand how they are set up. Using the “User Upload” alternative, you furthermore...convenient for you to later identify how you were setting up the experiment.  Output_NotFoundList: A text...approach is to use smaller libraries targeting subsets of genes. With such an approach, you lose the unbiased...differentially expressed genes found in a gene expression dataset are causal for a phenotype of interest. Selected...

...summer role was incredibly valuable because it was a set duration of only three months and made a good job... in a developing tropical country. This is why I set up a placement working in a rural health clinic in... can be a glut of individuals with similar skill sets. This is not to say that all commercial companies...

...Without moving past the maximum possible setting on the pipette, set the desired volume of the liquid on the...tip is properly set on the pipette to ensure pipetting accuracy. Once the pipette is set and you have the... pipette tip, the volume display which shows the set volume for the the pipette, and the tip ejector ring...