...GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes (ex: ClaI...CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes that are...

...for career skills. Download a summary PDF here or try out the online Researcher Development Framework Planner...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the ...

...entire series as it is published. For more reading try The Discipline of Teams by Jon R. Katzenbach and ...

...freezer. No more issues deciphering handwriting or trying to find to where the binder was moved. You’ll have...and you can’t remember where you put your flow cytometry protocol. Or maybe you can’t find the molecular...

...growth after overnight incubation. What went wrong? Try growing the culture for more time. Some bacterial...following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If...

...unpublished evidence shows otherwise. Others will go on to try to apply these findings in their own work ultimately...likely that we’ll find a fluke when we’re simply trying to get our experiments to work, but part of good... sciences and featured speakers from academia, industry, nonprofits, and publishing. The livestream video...publishing community views reproducibility, how industry relies on reproducibility, and how you can push...

...one you use, of course, depends on what you are trying to do – there’s no “best” tool among them. While...best” gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or ...Rees HA, Liu DR (2018) Base editing: precision chemistry on the genome and transcriptome of living cells...

...or two hanging out around the lab and especially try to attend a lab meeting. Observe how the lab members...scientific meetings (and present)? Making the decision Try to rotate or interview in more than one lab when ...(topic and/or technical aspects) applicable to industry if this path interests you  (e.g. human disease...drug/device development? Does the advisor have industry connections such as serving on Boards? Does the...the publication record show evidence of industry collaboration? If you are considering a career at a small...

...secondary antibody may be binding non-specifically – try optimizing your blocking and washing conditions or...are always new and improved variations you could try. We hope this post helped you start planning for ...referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...antibodies available at Addgene Protocols for Immunocytochemistry Protocols for other antibody applications...

...sensitivity, which can be problematic if you are trying to show that your antibody can distinguish between...Additional resources on the Addgene blog:  Still trying to choose an antibody? Check out our Selecting ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....recognized by your antibody. You perform immunocytochemistry on these mutant cells as well as a non-mutant...epitopes of this protein.   You perform flow cytometry using both antibodies, as well as a third unrelated...sample using the antibody and then uses mass spectrometry to identify the captured proteins. If the antibody...

...highlighted in red above. When searching, it is worth trying alternate names, particularly for gene names. If...

...in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

...Your Science Why Scientists Should Give Twitter a Try ...

...service of COVID-19 research? We decided to give it a try, by creating a service for COVID-19 researchers. ...

...plasmids out there Beyond these 3 big pushes, we try to increase your work’s visibility in a few other...

...with high marker expression level. Labs have been trying different approaches to improve CTC capture, but... is small, highly stable and has this amazing chemistry locking the domain together - no one knows what.... Many other Gram-positive bacteria have this chemistry in their surface proteins. It is best studied ...

...fewer and fewer people use plastic bags.  We also try to recycle our discards according to the Cambridge...plastic materials are fairly inexpensive in this country and we are quick to throw out chipped or imperfect...

...site (MCS), but does not cut your insert, you could try using two different enzymes that have compatible ...

...do controls. See Tips and FAQ below for details. Try different vector to insert ratios to optimize the...

...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the applications common...