We narrowed to 997 results for: lars;

...drug to the market usually takes over a billion dollars and at least a decade of work. The number of steps... Where publication is expected, the process is similar to academia. The only difference is that your paper...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections... This helps avoid multiple freeze/thaws of your larger aliquots of virus. AAV titration by qPCR Overview...in vitro and in vivo use. Current protocols in molecular biology, Chapter 16, Unit 16.25. PubMed PMID: ...

...yourself anymore. Personally I found this to be particularly challenging. As PhD students and postdocs we...there is not a clear deadline. Finally check-in regularly, make yourself available, and encourage questions...manager for advice if you’re not sure how to face a particular situation. But keep in mind that a good manager...

...moist and hopefully aerated with a thin layer of molecular biology-grade agarose containing physiological... advantage of this technique is the potentially large number of single clones obtained (on occasion we...Maryland Medical School in Baltimore. She is particularly interested in signaling peptide maturation in...

...Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it has facilitated...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch...technologies. To date his group has develop several popular CRISPR-based tools for altering DNA sequence or...

...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...whole-plasmid integration if you’d like to integrate a larger amount of material into the genome. Open questions...

... being used. After learning of it's continued popularity, I decided this protocol would be a worthy first...Addgene has also cataloged a variety of useful molecular cloning protocols, including making LB agar plates...needs. At Addgene, we’ll continue to create new molecular biology educational resources, such as protocols...

...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...blade Plastic tray Gel imaging system ImageJ or similar software Reagents Purified recombinant antibody...should be very little background staining. Pro-Tip A large shift in the electrophoretic mobility of the bands...Analysis Use ImageJ (Link opens in a new window) or a similar photo software to determine the relative intensity...

... Thermo Scientific NanoDrop is one of the most popular options, so most of this post is based on reading... from a NanoDrop. The principles will apply to similar instruments from other companies, like the Implen...reagents, making it more difficult to detect. A particularly strong absorber at 230 nm is guanidine thiocyanate...

...hexon), as well as other intracellularly-exposed proteins and downstream cellular events, all recruit strong...Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector infected ...