We narrowed to 938 results for: Kin
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TypeBlog Post...box above. The number of action potential (AP) spiking events are indicated below the trace; asterisk ... now available through Addgene. Whether you're working with cell cultures, slice preparations, or in vivo...
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Fluorescent Proteins 101: Luciferases
TypeBlog Post... light Chemical reaction High-energy photons Kinetics of photon generation Slower Faster Cofactors...and more. These variant proteins offer different kinetics, substrate requirements, and photon emission wavelengths... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot...signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs... -
Clear and Accessible Writing
TypeBlog Post...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around the...helpfully. You and your readers are now a team, working together to share information. Sometimes it is ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...behind. While commercial kits are available for making small point mutations, large deletions/insertions...-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which the phage... -
Plasmids 101: Blue-white Screening
TypeBlog Post... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22... -
Plasmids 101: Yeast Vectors
TypeBlog Post...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post... STED nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment ...approach allows minute-by-minute analysis of the kinetics of chromatin regulation and subsequent mathematical...