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We narrowed to 1,073 results for: TOR;

Showing: 711 - 720 of 1073 results
  1. CRISPR in the Clinic

    Type
    Blog Post
    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...suite, with Beam Therapeutics initiating a base editor-based clinical trial for SCD in 2022 and Editas... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...
  2. A Look at Addgene's QC Process

    Type
    Blog Post
    ...compared to the information provided by the depositor. If a depositor provides a sequence for the plasmid, QC...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains... elements based on the information provided and vector type  Any tags, selectable markers, and fusion ...
  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ...depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi using four-dimensional contrast...assembly line for the creation of CRISPR targeting vectors that each contains a guide RNA and repair template...  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for introducing point mutations with unc- 119 +...
  4. 6 Tips for Grant Writing

    Type
    Blog Post
    ...screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor/ a previous supervisor / someone...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...Networking for Scientists Series 4. Become a science communicator More and more fellowship applications are asking...on your grant application to your peers and collaborators can also help you get some critical insight ...
  5. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...design was conceived by Mike Morrison, a psychology doctoral student at Michigan State University. The idea... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...be the focus of your poster - the data and your story. If you choose a single-color background but want...
  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...subcloned (using FX cloning) into the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag... and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound spike... Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101....16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L...
  7. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...to) feel valued. Talk to your manager and find a mentor Your manager should support you, just like you ...reaching out to your manager. This is when having a mentor - someone more experienced to guide you - is valuable...Management for Scientists Series What Makes a Good Mentor? Tips for Working in Industry and Success in Management...
  8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...daytime. They mostly stop emerging at night. An incubator with a light cycle makes for very predictable ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that...research moving. Below, you’ll find some great introductory resources. As you dive deeper into Drosophila... the upper right hand corner. They have video tutorials under the help tab like this one to get you started...
  9. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...thankfully, most competent cells do not express this factor. Zeocin is handy because it can be a one-stop shop...Hadley, W. K., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial...bonded proteins in its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-...
  10. Technical Design of a Western Blot

    Type
    Blog Post
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...
Showing: 711 - 720 of 1073 results