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... matter how many times I say this, candidates regularly try to convince me that their search committee...whiskies, but I remember the days of modest postdoc salaries all too well. If there's a group to make money...search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

..., but I always seek out information about the particular audience and venue before tailoring each presentation...peer mentors to check your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities...informal poll by asking for a show of hands on a particular question. I like to ask about how many people...

...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use...

...appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but with some extra...selecting an antibody, some of the requirements are similar to standard IF. Here, we’ll walk you through the...if the localization and intensity patterns are similar between the two experiments. If not, it might be...

...Mentorship Program work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant...President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as... Medical School, where she studies age-related macular degeneration. She is also a blogger who writes ...

... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

...samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction enzyme -...you get better separation of bands? If you have similarly sized bands that are running too close together..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

...result in similar sized bands, thus making this simple digest less informative. This is particularly true ...occurring bacterial endonucleases that recognize a large range of DNA sequences. Given the variety of these...

...less efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea...very few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically...